Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source
- Autores
- Valdez, Alejandra Leonor; Viñarta, Silvana Carolina; Castellanos, Lucia Ines; Fariña, Julia Ines
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Scleroglucan is a β-D-(1,3)-(1,6)-glucan produced by Sclerotium rolfsii ATCC 201126. This neutral water-soluble exopolysaccharide (EPS) was produced by submerged culture in MP20 liquid medium (sucrose: 20 g/L; initial pH: 4.5). The influence of 9 different simple and complex carbon sources on scleroglucan, biomass and oxalic acid production was evaluated. Batch fermentation processes were carried out in Erlenmeyer flasks at 30ºC and 250 rpm during 72 h. Higher EPS production (7 g/L) was obtained with sucrose and maltose as C-source. Biomass was significantly increased with maltose (~9 g/L) and soluble starch (9.6 g/L). Variable concentrations of oxalic acid were observed depending on the C-source used. The possibility to select appropriated culture conditions for S. rolfsii ATCC 201126 according to the product of interest was demonstrated. Additionally, biomass and EPS production obtained with molasses as C-source would allow to propose the use of low-cost substrates for the production of high-added value metabolites.
Fil: Valdez, Alejandra Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular - Materia
-
Scleroglucan production
Complex carbon source
Oxalic acid - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/213133
Ver los metadatos del registro completo
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Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon sourceValdez, Alejandra LeonorViñarta, Silvana CarolinaCastellanos, Lucia InesFariña, Julia InesScleroglucan productionComplex carbon sourceOxalic acidhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Scleroglucan is a β-D-(1,3)-(1,6)-glucan produced by Sclerotium rolfsii ATCC 201126. This neutral water-soluble exopolysaccharide (EPS) was produced by submerged culture in MP20 liquid medium (sucrose: 20 g/L; initial pH: 4.5). The influence of 9 different simple and complex carbon sources on scleroglucan, biomass and oxalic acid production was evaluated. Batch fermentation processes were carried out in Erlenmeyer flasks at 30ºC and 250 rpm during 72 h. Higher EPS production (7 g/L) was obtained with sucrose and maltose as C-source. Biomass was significantly increased with maltose (~9 g/L) and soluble starch (9.6 g/L). Variable concentrations of oxalic acid were observed depending on the C-source used. The possibility to select appropriated culture conditions for S. rolfsii ATCC 201126 according to the product of interest was demonstrated. Additionally, biomass and EPS production obtained with molasses as C-source would allow to propose the use of low-cost substrates for the production of high-added value metabolites.Fil: Valdez, Alejandra Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213133Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-930327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:42Zoai:ri.conicet.gov.ar:11336/213133instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:43.207CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
title |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
spellingShingle |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source Valdez, Alejandra Leonor Scleroglucan production Complex carbon source Oxalic acid |
title_short |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
title_full |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
title_fullStr |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
title_full_unstemmed |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
title_sort |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source |
dc.creator.none.fl_str_mv |
Valdez, Alejandra Leonor Viñarta, Silvana Carolina Castellanos, Lucia Ines Fariña, Julia Ines |
author |
Valdez, Alejandra Leonor |
author_facet |
Valdez, Alejandra Leonor Viñarta, Silvana Carolina Castellanos, Lucia Ines Fariña, Julia Ines |
author_role |
author |
author2 |
Viñarta, Silvana Carolina Castellanos, Lucia Ines Fariña, Julia Ines |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Scleroglucan production Complex carbon source Oxalic acid |
topic |
Scleroglucan production Complex carbon source Oxalic acid |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Scleroglucan is a β-D-(1,3)-(1,6)-glucan produced by Sclerotium rolfsii ATCC 201126. This neutral water-soluble exopolysaccharide (EPS) was produced by submerged culture in MP20 liquid medium (sucrose: 20 g/L; initial pH: 4.5). The influence of 9 different simple and complex carbon sources on scleroglucan, biomass and oxalic acid production was evaluated. Batch fermentation processes were carried out in Erlenmeyer flasks at 30ºC and 250 rpm during 72 h. Higher EPS production (7 g/L) was obtained with sucrose and maltose as C-source. Biomass was significantly increased with maltose (~9 g/L) and soluble starch (9.6 g/L). Variable concentrations of oxalic acid were observed depending on the C-source used. The possibility to select appropriated culture conditions for S. rolfsii ATCC 201126 according to the product of interest was demonstrated. Additionally, biomass and EPS production obtained with molasses as C-source would allow to propose the use of low-cost substrates for the production of high-added value metabolites. Fil: Valdez, Alejandra Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular Potrero de los Funes Argentina Sociedad Argentina de Investigación en Bioquímica y Biología Molecular |
description |
Scleroglucan is a β-D-(1,3)-(1,6)-glucan produced by Sclerotium rolfsii ATCC 201126. This neutral water-soluble exopolysaccharide (EPS) was produced by submerged culture in MP20 liquid medium (sucrose: 20 g/L; initial pH: 4.5). The influence of 9 different simple and complex carbon sources on scleroglucan, biomass and oxalic acid production was evaluated. Batch fermentation processes were carried out in Erlenmeyer flasks at 30ºC and 250 rpm during 72 h. Higher EPS production (7 g/L) was obtained with sucrose and maltose as C-source. Biomass was significantly increased with maltose (~9 g/L) and soluble starch (9.6 g/L). Variable concentrations of oxalic acid were observed depending on the C-source used. The possibility to select appropriated culture conditions for S. rolfsii ATCC 201126 according to the product of interest was demonstrated. Additionally, biomass and EPS production obtained with molasses as C-source would allow to propose the use of low-cost substrates for the production of high-added value metabolites. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/213133 Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/213133 |
identifier_str_mv |
Scleroglucan production by Sclerotium rolfsii ATCC 201126: Influence of the carbon source; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 93-93 0327-9545 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083079661158400 |
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13.22299 |