Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats

Autores
Jammal, María Victoria; Fariña, Julia Ines; Pastorino, Nina Fiorella; Missana, Liliana Raquel
Año de publicación
2015
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biología
Materia
BIOCOMPATIBILITY
SCLEROGLUCAN
TISSUE ENGINEERING
RATS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189547
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189547
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of ratsJammal, María VictoriaFariña, Julia InesPastorino, Nina FiorellaMissana, Liliana RaquelBIOCOMPATIBILITYSCLEROGLUCANTISSUE ENGINEERINGRATShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology SocietySan Miguel de TucumánArgentinaSociedad Argentina de BiologíaTech Science Press2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189547Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-21667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/descargas/jornadas/Biocell-39-Supl-5-2015_SAB-2015.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:45Zoai:ri.conicet.gov.ar:11336/189547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:45.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
title Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
spellingShingle Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
Jammal, María Victoria
BIOCOMPATIBILITY
SCLEROGLUCAN
TISSUE ENGINEERING
RATS
title_short Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
title_full Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
title_fullStr Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
title_full_unstemmed Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
title_sort Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
dc.creator.none.fl_str_mv Jammal, María Victoria
Fariña, Julia Ines
Pastorino, Nina Fiorella
Missana, Liliana Raquel
author Jammal, María Victoria
author_facet Jammal, María Victoria
Fariña, Julia Ines
Pastorino, Nina Fiorella
Missana, Liliana Raquel
author_role author
author2 Fariña, Julia Ines
Pastorino, Nina Fiorella
Missana, Liliana Raquel
author2_role author
author
author
dc.subject.none.fl_str_mv BIOCOMPATIBILITY
SCLEROGLUCAN
TISSUE ENGINEERING
RATS
topic BIOCOMPATIBILITY
SCLEROGLUCAN
TISSUE ENGINEERING
RATS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biología
description Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189547
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-2
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189547
identifier_str_mv Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-2
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/descargas/jornadas/Biocell-39-Supl-5-2015_SAB-2015.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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