Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats
- Autores
- Jammal, María Victoria; Fariña, Julia Ines; Pastorino, Nina Fiorella; Missana, Liliana Raquel
- Año de publicación
- 2015
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biología - Materia
-
BIOCOMPATIBILITY
SCLEROGLUCAN
TISSUE ENGINEERING
RATS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/189547
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Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of ratsJammal, María VictoriaFariña, Julia InesPastorino, Nina FiorellaMissana, Liliana RaquelBIOCOMPATIBILITYSCLEROGLUCANTISSUE ENGINEERINGRATShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment.Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology SocietySan Miguel de TucumánArgentinaSociedad Argentina de BiologíaTech Science Press2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189547Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-21667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/descargas/jornadas/Biocell-39-Supl-5-2015_SAB-2015.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:45Zoai:ri.conicet.gov.ar:11336/189547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:45.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
title |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
spellingShingle |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats Jammal, María Victoria BIOCOMPATIBILITY SCLEROGLUCAN TISSUE ENGINEERING RATS |
title_short |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
title_full |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
title_fullStr |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
title_full_unstemmed |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
title_sort |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats |
dc.creator.none.fl_str_mv |
Jammal, María Victoria Fariña, Julia Ines Pastorino, Nina Fiorella Missana, Liliana Raquel |
author |
Jammal, María Victoria |
author_facet |
Jammal, María Victoria Fariña, Julia Ines Pastorino, Nina Fiorella Missana, Liliana Raquel |
author_role |
author |
author2 |
Fariña, Julia Ines Pastorino, Nina Fiorella Missana, Liliana Raquel |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
BIOCOMPATIBILITY SCLEROGLUCAN TISSUE ENGINEERING RATS |
topic |
BIOCOMPATIBILITY SCLEROGLUCAN TISSUE ENGINEERING RATS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment. Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Pastorino, Nina Fiorella. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society San Miguel de Tucumán Argentina Sociedad Argentina de Biología |
description |
Bone Tissue Engineering (BTE) combines three-dimensional scaffolds (3D), cells and signaling molecules to repair bone through mature engineered tissue. Our objective was to evaluate the in vivo biological response of a natural polymer scleroglucan called S. rolfsii ATCC 201126 implanted into rat subcutaneous tissues (ST) in order to consider its use combined with rhPTH scaffold for BTE. Polymer and zinc oxide eugenol pellets were implanted into the ST of 30 Wistar rats (150 ± 50 g). Animals were sacrificed at 7 and 30 days, and samples from implanted material and surrounded tissues were fixed in 10% buffered formalin. They were included in paraffin, serial cut and stained with H&E stain. At 7 days the polymer surrounded and colonized by polymorphonuclear neutrophils (PMNn) was observed. A lympho-mononuclear infiltrate was also found. At the interface between polymer and ST granulation tissue, many fibroblasts and new vessels, surrounded by fibrous tissue were observed. Zinc oxide eugenol presented fibrin leukocyte exudation and coagulation necrosis, surrounded by loose connective tissue. At 30 days the polymer was resorbed and replaced by fibrous connective tissue. However, zinc oxide eugenol was surrounded by dense fibrous connective tissue-like capsule. The results demonstrated that scleroglucan S. rolfsii ATCC 201126 was biocompatible and bioresorbable in rat ST, supporting its application as a scaffold in BTE. In addition, the natural origin of this polymer makes it compatible with the environment. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/189547 Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-2 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/189547 |
identifier_str_mv |
Biocompatibility evaluation of Sclerotium rolfsii ATCC 201126 scleroglucan in subcutaneous tissue of rats; XXXII Annual Scientific Meeting of the Tucumán Biology Association; XX Annual Scientific Meeting of the Córdoba Biology Society; XXXIII Annual Scientific Meeting of the Cuyo Biology Society and XVII Annual Meeting of the Argentine Biology Society; San Miguel de Tucumán; Argentina; 2015; 1-2 1667-5746 CONICET Digital CONICET |
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spa |
language |
spa |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/descargas/jornadas/Biocell-39-Supl-5-2015_SAB-2015.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Nacional |
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Tech Science Press |
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Tech Science Press |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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