Participation of GABA transporter in immune response and neuro-immune communication

Autores
Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose
Año de publicación
2015
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The nervous and the immune systems (NS and IS respectively) are physically and physiologically connected. Recently, expression of neurotransmitter system components in immune cells and synthesis and receptors of cytokines in NS cells were described. We previously reported that a complete GABAergic system is functionally expressed in human lymphocytes. Now, we are focusing on GABA transporters (GATs). Four GAT subtypes (GAT 1-3 and BGT-1) were described in human NS. We studied GAT mRNA levels in activated and resting lymphocytes (with and without the mitogen phytohemagglutinin (PHA), respectively). GAT-2 and BGT-1 mRNAs were detected in most of activated cells. Moreover, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. We also analyzed GABA levels in lymphocyte cultures. We could only detect GABA in supernatant from activated cells. Despite its typical role in the synapse where they mediate cellular uptake of GABA, under certain conditions GATs can reverse and release GABA. This secretion is vesicle independent. We propose that this mechanism could be involved in GABA release in lymphocytes. Establishing the role of endogenous GATs in immune response and as a link between NS and IS will provide new therapeutic targets for the treatment of diseases that could affect both systems
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXX Reunión Anual de Sociedad Argentina de Neuroquímica
Mar del Plata
Argentina
Sociedad Argentina de Neuroquímica
Materia
GABA
TRANSPORTERS
IMMUNE SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237476
Acceder
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spelling Participation of GABA transporter in immune response and neuro-immune communicationDionisio, Leonardo RaulCaldironi, Hugo Alfredode Rosa, Maria JoseGABATRANSPORTERSIMMUNE SYSTEMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nervous and the immune systems (NS and IS respectively) are physically and physiologically connected. Recently, expression of neurotransmitter system components in immune cells and synthesis and receptors of cytokines in NS cells were described. We previously reported that a complete GABAergic system is functionally expressed in human lymphocytes. Now, we are focusing on GABA transporters (GATs). Four GAT subtypes (GAT 1-3 and BGT-1) were described in human NS. We studied GAT mRNA levels in activated and resting lymphocytes (with and without the mitogen phytohemagglutinin (PHA), respectively). GAT-2 and BGT-1 mRNAs were detected in most of activated cells. Moreover, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. We also analyzed GABA levels in lymphocyte cultures. We could only detect GABA in supernatant from activated cells. Despite its typical role in the synapse where they mediate cellular uptake of GABA, under certain conditions GATs can reverse and release GABA. This secretion is vesicle independent. We propose that this mechanism could be involved in GABA release in lymphocytes. Establishing the role of endogenous GATs in immune response and as a link between NS and IS will provide new therapeutic targets for the treatment of diseases that could affect both systemsFil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXX Reunión Anual de Sociedad Argentina de NeuroquímicaMar del PlataArgentinaSociedad Argentina de NeuroquímicaBuenos Aires2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237476Participation of GABA transporter in immune response and neuro-immune communication; XXX Reunión Anual de Sociedad Argentina de Neuroquímica; Mar del Plata; Argentina; 2015; 306-306CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:28Zoai:ri.conicet.gov.ar:11336/237476instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:28.807CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Participation of GABA transporter in immune response and neuro-immune communication
title Participation of GABA transporter in immune response and neuro-immune communication
spellingShingle Participation of GABA transporter in immune response and neuro-immune communication
Dionisio, Leonardo Raul
GABA
TRANSPORTERS
IMMUNE SYSTEM
title_short Participation of GABA transporter in immune response and neuro-immune communication
title_full Participation of GABA transporter in immune response and neuro-immune communication
title_fullStr Participation of GABA transporter in immune response and neuro-immune communication
title_full_unstemmed Participation of GABA transporter in immune response and neuro-immune communication
title_sort Participation of GABA transporter in immune response and neuro-immune communication
dc.creator.none.fl_str_mv Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author Dionisio, Leonardo Raul
author_facet Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author_role author
author2 Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author2_role author
author
dc.subject.none.fl_str_mv GABA
TRANSPORTERS
IMMUNE SYSTEM
topic GABA
TRANSPORTERS
IMMUNE SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The nervous and the immune systems (NS and IS respectively) are physically and physiologically connected. Recently, expression of neurotransmitter system components in immune cells and synthesis and receptors of cytokines in NS cells were described. We previously reported that a complete GABAergic system is functionally expressed in human lymphocytes. Now, we are focusing on GABA transporters (GATs). Four GAT subtypes (GAT 1-3 and BGT-1) were described in human NS. We studied GAT mRNA levels in activated and resting lymphocytes (with and without the mitogen phytohemagglutinin (PHA), respectively). GAT-2 and BGT-1 mRNAs were detected in most of activated cells. Moreover, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. We also analyzed GABA levels in lymphocyte cultures. We could only detect GABA in supernatant from activated cells. Despite its typical role in the synapse where they mediate cellular uptake of GABA, under certain conditions GATs can reverse and release GABA. This secretion is vesicle independent. We propose that this mechanism could be involved in GABA release in lymphocytes. Establishing the role of endogenous GATs in immune response and as a link between NS and IS will provide new therapeutic targets for the treatment of diseases that could affect both systems
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXX Reunión Anual de Sociedad Argentina de Neuroquímica
Mar del Plata
Argentina
Sociedad Argentina de Neuroquímica
description The nervous and the immune systems (NS and IS respectively) are physically and physiologically connected. Recently, expression of neurotransmitter system components in immune cells and synthesis and receptors of cytokines in NS cells were described. We previously reported that a complete GABAergic system is functionally expressed in human lymphocytes. Now, we are focusing on GABA transporters (GATs). Four GAT subtypes (GAT 1-3 and BGT-1) were described in human NS. We studied GAT mRNA levels in activated and resting lymphocytes (with and without the mitogen phytohemagglutinin (PHA), respectively). GAT-2 and BGT-1 mRNAs were detected in most of activated cells. Moreover, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. We also analyzed GABA levels in lymphocyte cultures. We could only detect GABA in supernatant from activated cells. Despite its typical role in the synapse where they mediate cellular uptake of GABA, under certain conditions GATs can reverse and release GABA. This secretion is vesicle independent. We propose that this mechanism could be involved in GABA release in lymphocytes. Establishing the role of endogenous GATs in immune response and as a link between NS and IS will provide new therapeutic targets for the treatment of diseases that could affect both systems
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237476
Participation of GABA transporter in immune response and neuro-immune communication; XXX Reunión Anual de Sociedad Argentina de Neuroquímica; Mar del Plata; Argentina; 2015; 306-306
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237476
identifier_str_mv Participation of GABA transporter in immune response and neuro-immune communication; XXX Reunión Anual de Sociedad Argentina de Neuroquímica; Mar del Plata; Argentina; 2015; 306-306
CONICET Digital
CONICET
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language spa
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Buenos Aires
publisher.none.fl_str_mv Buenos Aires
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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