GABA transporters in human lymphocytes

Autores
de Rosa, Maria Jose; Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; Bouzat, Cecilia Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
GABA is the main inhibitory neurotransmitter in CNS and is associated to several neurological disorders. GABA transporters (GATs) play a critical role in GABA level regulation by allowing re-uptake of neuronal secreted GABA. Four GAT subtypes (GAT 1-3 and BGT-1) have been described in humans. Previously, we reported a complete GABAergic system in lymphocytes. Here, we studied the modulation of the expression and activity of GATs in human lymphocytes by the mitogen phytohemagglutinin (PHA). We determined mRNA GAT expression in activated and resting cells (with and without PHA, respectively). GAT 3 was not detected under any condition, whereas GAT 2 and BGT-1 were detected in all activated cells. Expression of GAT 1 was variable among samples and conditions. In line with these observations, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. In addition, secreted GABA was detected only in supernatant from activated lymphocyte cultures. Taken together, our results show that lymphocytes express functional GATs whose expression is modulated by PHA, GATs regulate cell proliferation, and lymphocyte cells have the ability to secrete GABA. Pharmacological modulation of GATs present in lymphocytes could be a new target to modulate immune response.
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
GABA TRANSPORTERS
LYMPHOCYTES
GABA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235378
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GABA transporters in human lymphocytesde Rosa, Maria JoseDionisio, Leonardo RaulCaldironi, Hugo AlfredoBouzat, Cecilia BeatrizGABA TRANSPORTERSLYMPHOCYTESGABAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1GABA is the main inhibitory neurotransmitter in CNS and is associated to several neurological disorders. GABA transporters (GATs) play a critical role in GABA level regulation by allowing re-uptake of neuronal secreted GABA. Four GAT subtypes (GAT 1-3 and BGT-1) have been described in humans. Previously, we reported a complete GABAergic system in lymphocytes. Here, we studied the modulation of the expression and activity of GATs in human lymphocytes by the mitogen phytohemagglutinin (PHA). We determined mRNA GAT expression in activated and resting cells (with and without PHA, respectively). GAT 3 was not detected under any condition, whereas GAT 2 and BGT-1 were detected in all activated cells. Expression of GAT 1 was variable among samples and conditions. In line with these observations, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. In addition, secreted GABA was detected only in supernatant from activated lymphocyte cultures. Taken together, our results show that lymphocytes express functional GATs whose expression is modulated by PHA, GATs regulate cell proliferation, and lymphocyte cells have the ability to secrete GABA. Pharmacological modulation of GATs present in lymphocytes could be a new target to modulate immune response.Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor MaldonadoArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/235378GABA transporters in human lymphocytes; XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado; Argentina; 2013; 311-311CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:29Zoai:ri.conicet.gov.ar:11336/235378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:29.98CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GABA transporters in human lymphocytes
title GABA transporters in human lymphocytes
spellingShingle GABA transporters in human lymphocytes
de Rosa, Maria Jose
GABA TRANSPORTERS
LYMPHOCYTES
GABA
title_short GABA transporters in human lymphocytes
title_full GABA transporters in human lymphocytes
title_fullStr GABA transporters in human lymphocytes
title_full_unstemmed GABA transporters in human lymphocytes
title_sort GABA transporters in human lymphocytes
dc.creator.none.fl_str_mv de Rosa, Maria Jose
Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
Bouzat, Cecilia Beatriz
author de Rosa, Maria Jose
author_facet de Rosa, Maria Jose
Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
Bouzat, Cecilia Beatriz
author_role author
author2 Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
Bouzat, Cecilia Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv GABA TRANSPORTERS
LYMPHOCYTES
GABA
topic GABA TRANSPORTERS
LYMPHOCYTES
GABA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv GABA is the main inhibitory neurotransmitter in CNS and is associated to several neurological disorders. GABA transporters (GATs) play a critical role in GABA level regulation by allowing re-uptake of neuronal secreted GABA. Four GAT subtypes (GAT 1-3 and BGT-1) have been described in humans. Previously, we reported a complete GABAergic system in lymphocytes. Here, we studied the modulation of the expression and activity of GATs in human lymphocytes by the mitogen phytohemagglutinin (PHA). We determined mRNA GAT expression in activated and resting cells (with and without PHA, respectively). GAT 3 was not detected under any condition, whereas GAT 2 and BGT-1 were detected in all activated cells. Expression of GAT 1 was variable among samples and conditions. In line with these observations, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. In addition, secreted GABA was detected only in supernatant from activated lymphocyte cultures. Taken together, our results show that lymphocytes express functional GATs whose expression is modulated by PHA, GATs regulate cell proliferation, and lymphocyte cells have the ability to secrete GABA. Pharmacological modulation of GATs present in lymphocytes could be a new target to modulate immune response.
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado
Argentina
Sociedad Argentina de Investigación en Neurociencias
description GABA is the main inhibitory neurotransmitter in CNS and is associated to several neurological disorders. GABA transporters (GATs) play a critical role in GABA level regulation by allowing re-uptake of neuronal secreted GABA. Four GAT subtypes (GAT 1-3 and BGT-1) have been described in humans. Previously, we reported a complete GABAergic system in lymphocytes. Here, we studied the modulation of the expression and activity of GATs in human lymphocytes by the mitogen phytohemagglutinin (PHA). We determined mRNA GAT expression in activated and resting cells (with and without PHA, respectively). GAT 3 was not detected under any condition, whereas GAT 2 and BGT-1 were detected in all activated cells. Expression of GAT 1 was variable among samples and conditions. In line with these observations, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. In addition, secreted GABA was detected only in supernatant from activated lymphocyte cultures. Taken together, our results show that lymphocytes express functional GATs whose expression is modulated by PHA, GATs regulate cell proliferation, and lymphocyte cells have the ability to secrete GABA. Pharmacological modulation of GATs present in lymphocytes could be a new target to modulate immune response.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235378
GABA transporters in human lymphocytes; XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado; Argentina; 2013; 311-311
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235378
identifier_str_mv GABA transporters in human lymphocytes; XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado; Argentina; 2013; 311-311
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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