Expression and function of non-neuronal GABA transporters in human lymphocytes

Autores
Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
GABA
GABA TRANSPORTERS
GAT EXPRESSION
LYMPHOCYTES
NEUROIMMUNE INTERACTIONS
NON-NEURONAL TISSUES
PHARMACOLOGICAL MODULATION
T-CELL PROLIFERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63080
Acceder
id CONICETDig_8db7386b1cce570135aadfd612ffc572
oai_identifier_str oai:ri.conicet.gov.ar:11336/63080
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and function of non-neuronal GABA transporters in human lymphocytesDionisio, Leonardo RaulCaldironi, Hugo Alfredode Rosa, Maria JoseGABAGABA TRANSPORTERSGAT EXPRESSIONLYMPHOCYTESNEUROIMMUNE INTERACTIONSNON-NEURONAL TISSUESPHARMACOLOGICAL MODULATIONT-CELL PROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAsian Network for Scientific Information2017-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63080Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-2111811-77751812-5700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=ijp.2017.205.211info:eu-repo/semantics/altIdentifier/doi/10.3923/ijp.2017.205.211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:10:48Zoai:ri.conicet.gov.ar:11336/63080instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:10:49.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and function of non-neuronal GABA transporters in human lymphocytes
title Expression and function of non-neuronal GABA transporters in human lymphocytes
spellingShingle Expression and function of non-neuronal GABA transporters in human lymphocytes
Dionisio, Leonardo Raul
GABA
GABA TRANSPORTERS
GAT EXPRESSION
LYMPHOCYTES
NEUROIMMUNE INTERACTIONS
NON-NEURONAL TISSUES
PHARMACOLOGICAL MODULATION
T-CELL PROLIFERATION
title_short Expression and function of non-neuronal GABA transporters in human lymphocytes
title_full Expression and function of non-neuronal GABA transporters in human lymphocytes
title_fullStr Expression and function of non-neuronal GABA transporters in human lymphocytes
title_full_unstemmed Expression and function of non-neuronal GABA transporters in human lymphocytes
title_sort Expression and function of non-neuronal GABA transporters in human lymphocytes
dc.creator.none.fl_str_mv Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author Dionisio, Leonardo Raul
author_facet Dionisio, Leonardo Raul
Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author_role author
author2 Caldironi, Hugo Alfredo
de Rosa, Maria Jose
author2_role author
author
dc.subject.none.fl_str_mv GABA
GABA TRANSPORTERS
GAT EXPRESSION
LYMPHOCYTES
NEUROIMMUNE INTERACTIONS
NON-NEURONAL TISSUES
PHARMACOLOGICAL MODULATION
T-CELL PROLIFERATION
topic GABA
GABA TRANSPORTERS
GAT EXPRESSION
LYMPHOCYTES
NEUROIMMUNE INTERACTIONS
NON-NEURONAL TISSUES
PHARMACOLOGICAL MODULATION
T-CELL PROLIFERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63080
Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-211
1811-7775
1812-5700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63080
identifier_str_mv Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-211
1811-7775
1812-5700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=ijp.2017.205.211
info:eu-repo/semantics/altIdentifier/doi/10.3923/ijp.2017.205.211
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asian Network for Scientific Information
publisher.none.fl_str_mv Asian Network for Scientific Information
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083256669175808
score 13.22299

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