Expression and function of non-neuronal GABA transporters in human lymphocytes
- Autores
- Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina - Materia
-
GABA
GABA TRANSPORTERS
GAT EXPRESSION
LYMPHOCYTES
NEUROIMMUNE INTERACTIONS
NON-NEURONAL TISSUES
PHARMACOLOGICAL MODULATION
T-CELL PROLIFERATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/63080
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/63080 |
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Expression and function of non-neuronal GABA transporters in human lymphocytesDionisio, Leonardo RaulCaldironi, Hugo Alfredode Rosa, Maria JoseGABAGABA TRANSPORTERSGAT EXPRESSIONLYMPHOCYTESNEUROIMMUNE INTERACTIONSNON-NEURONAL TISSUESPHARMACOLOGICAL MODULATIONT-CELL PROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies.Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAsian Network for Scientific Information2017-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63080Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-2111811-77751812-5700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=ijp.2017.205.211info:eu-repo/semantics/altIdentifier/doi/10.3923/ijp.2017.205.211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:43:40Zoai:ri.conicet.gov.ar:11336/63080instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:43:40.545CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
title |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
spellingShingle |
Expression and function of non-neuronal GABA transporters in human lymphocytes Dionisio, Leonardo Raul GABA GABA TRANSPORTERS GAT EXPRESSION LYMPHOCYTES NEUROIMMUNE INTERACTIONS NON-NEURONAL TISSUES PHARMACOLOGICAL MODULATION T-CELL PROLIFERATION |
title_short |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
title_full |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
title_fullStr |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
title_full_unstemmed |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
title_sort |
Expression and function of non-neuronal GABA transporters in human lymphocytes |
dc.creator.none.fl_str_mv |
Dionisio, Leonardo Raul Caldironi, Hugo Alfredo de Rosa, Maria Jose |
author |
Dionisio, Leonardo Raul |
author_facet |
Dionisio, Leonardo Raul Caldironi, Hugo Alfredo de Rosa, Maria Jose |
author_role |
author |
author2 |
Caldironi, Hugo Alfredo de Rosa, Maria Jose |
author2_role |
author author |
dc.subject.none.fl_str_mv |
GABA GABA TRANSPORTERS GAT EXPRESSION LYMPHOCYTES NEUROIMMUNE INTERACTIONS NON-NEURONAL TISSUES PHARMACOLOGICAL MODULATION T-CELL PROLIFERATION |
topic |
GABA GABA TRANSPORTERS GAT EXPRESSION LYMPHOCYTES NEUROIMMUNE INTERACTIONS NON-NEURONAL TISSUES PHARMACOLOGICAL MODULATION T-CELL PROLIFERATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies. Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Caldironi, Hugo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina |
description |
Background: Neurotransmitters not only play a key role in neuronal communication but also coordinate cell functions in non-neuronal cells, such as immune cells. Previous research reported a complete GABAergic system in human lymphocytes. In this study, focus is put on GABA transporters (GATs) and their physiological role during lymphocyte activation. Materials and Methods: Using RT-PCR and [3H]-GABA uptake assays, GAT expression and activity was evaluated under basal conditions and in cells exposed to phytohemagglutinin (PHA) or GABA. To study GAT role, cell proliferation was evaluated by [3H]-thymidine incorporation in the presence of Nipecotic Acid (NA), a GAT inhibitor. Finally, using HPLC GABA levels were analyzed in culture supernatants. Results: In lymphocytes under any condition, at least one GAT subtype was detected. No GAT-3 was found. The PHA and GABA-treatment increased both expression and activity. In addition, GAT blockade inhibited PHA-induced lymphocyte proliferation. The GABA was detected only in PHA-treated cell supernatants, thus indicating GABA secretion at least during proliferation. Conclusion: These findings demonstrate that extraneuronal GATs affect lymphocyte functionality. Characterization of GAT roles in immune response and as a link between nervous system and immune system will provide new therapeutic targets and could help reduce side effects of current therapies. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/63080 Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-211 1811-7775 1812-5700 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/63080 |
identifier_str_mv |
Dionisio, Leonardo Raul; Caldironi, Hugo Alfredo; de Rosa, Maria Jose; Expression and function of non-neuronal GABA transporters in human lymphocytes; Asian Network for Scientific Information; International Journal Of Pharmacology; 13; 2; 15-1-2017; 205-211 1811-7775 1812-5700 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://scialert.net/abstract/?doi=ijp.2017.205.211 info:eu-repo/semantics/altIdentifier/doi/10.3923/ijp.2017.205.211 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Asian Network for Scientific Information |
publisher.none.fl_str_mv |
Asian Network for Scientific Information |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846782129335173120 |
score |
13.229304 |