GABA modulation of olivocochlear efferent neurotransmission

Autores
Castagnola, Tais; Elgoyhen, Ana Belen; Goutman, Juan Diego; Wedemeyer, Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic fibers were used. Preliminary results indicate that ChR2 activation by light did not elicit any measurable synaptic response in IHC per se, but produced a transient potentiation of cholinergic synaptic responses (when coupled with an electrical stimulation) in 10/28 cases. In addition, immunohistochemistry techniques were used to characterize GABA innervation. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXIV Annual Meeting of the Argentinian Society of Neuroscience
Córdoba
Argentina
Sociedad Argentina de Neurociencia
Materia
OLIVOCOCHLEAR EFFERENT SYSTEM
COCHLEA
GABA
MODULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154485
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GABA modulation of olivocochlear efferent neurotransmissionCastagnola, TaisElgoyhen, Ana BelenGoutman, Juan DiegoWedemeyer, CarolinaOLIVOCOCHLEAR EFFERENT SYSTEMCOCHLEAGABAMODULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic fibers were used. Preliminary results indicate that ChR2 activation by light did not elicit any measurable synaptic response in IHC per se, but produced a transient potentiation of cholinergic synaptic responses (when coupled with an electrical stimulation) in 10/28 cases. In addition, immunohistochemistry techniques were used to characterize GABA innervation. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXXIV Annual Meeting of the Argentinian Society of NeuroscienceCórdobaArgentinaSociedad Argentina de NeurocienciaSAGE Publications2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154485GABA modulation of olivocochlear efferent neurotransmission; XXXIV Annual Meeting of the Argentinian Society of Neuroscience; Córdoba; Argentina; 2019; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718117/info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:02Zoai:ri.conicet.gov.ar:11336/154485instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:03.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GABA modulation of olivocochlear efferent neurotransmission
title GABA modulation of olivocochlear efferent neurotransmission
spellingShingle GABA modulation of olivocochlear efferent neurotransmission
Castagnola, Tais
OLIVOCOCHLEAR EFFERENT SYSTEM
COCHLEA
GABA
MODULATION
title_short GABA modulation of olivocochlear efferent neurotransmission
title_full GABA modulation of olivocochlear efferent neurotransmission
title_fullStr GABA modulation of olivocochlear efferent neurotransmission
title_full_unstemmed GABA modulation of olivocochlear efferent neurotransmission
title_sort GABA modulation of olivocochlear efferent neurotransmission
dc.creator.none.fl_str_mv Castagnola, Tais
Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author Castagnola, Tais
author_facet Castagnola, Tais
Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author_role author
author2 Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author2_role author
author
author
dc.subject.none.fl_str_mv OLIVOCOCHLEAR EFFERENT SYSTEM
COCHLEA
GABA
MODULATION
topic OLIVOCOCHLEAR EFFERENT SYSTEM
COCHLEA
GABA
MODULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic fibers were used. Preliminary results indicate that ChR2 activation by light did not elicit any measurable synaptic response in IHC per se, but produced a transient potentiation of cholinergic synaptic responses (when coupled with an electrical stimulation) in 10/28 cases. In addition, immunohistochemistry techniques were used to characterize GABA innervation. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXIV Annual Meeting of the Argentinian Society of Neuroscience
Córdoba
Argentina
Sociedad Argentina de Neurociencia
description During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic fibers were used. Preliminary results indicate that ChR2 activation by light did not elicit any measurable synaptic response in IHC per se, but produced a transient potentiation of cholinergic synaptic responses (when coupled with an electrical stimulation) in 10/28 cases. In addition, immunohistochemistry techniques were used to characterize GABA innervation. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154485
GABA modulation of olivocochlear efferent neurotransmission; XXXIV Annual Meeting of the Argentinian Society of Neuroscience; Córdoba; Argentina; 2019; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154485
identifier_str_mv GABA modulation of olivocochlear efferent neurotransmission; XXXIV Annual Meeting of the Argentinian Society of Neuroscience; Córdoba; Argentina; 2019; 1-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718117/
info:eu-repo/semantics/altIdentifier/doi/10.1177/1759091420979851
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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