In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza

Autores
Lobato Ureche, Miguel Andres; Pérez Rodriguez, María Micaela; Ortiz, R.; Lucero, Gabriela Susana; Cohen, Ana Carmen
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The rapid growth of the human population and their need of food has impulse to extensive use of chemical fertilizers and pesticides to increase yield crop. These practices are costs and increase environmental pollution. In the last years, appear needs for novel agricultural practices that do not harm natural ecosystems. Different plant growth promotion Rhizobacteria (PGPR) have been studied and incorporated into agricultural practices and biocontrol has emerged in recent years as an alternative to pesticides. PGPR antagonize orprevent the effects of phytopathogens or deleterious microorganisms. PGPR produce substances that protect them against variousdiseases. Metabolites include hydrogen cyanide and antibiotics. Some strain PGPRs produce lytic enzymes as β-1,3-glucanase,chitinase, protease and cellulase that degrade the cell wall of fungi and produce a direct inhibitory effect on the growth of hyphae.Another important mechanism of biocontrol of PGPRs is related to the production of siderophores that chelate iron, making itunavailable to pathogens. In this study four native PGPR strains of Mendoza against pathogenic fungi of pepper crops were evaluated.The test was carried out by fourfold and repeated two times using independent Petri dishes for the growth of each bacterial strain andeach pathogens. On Luria Broth (LB) and potato dextrose agar (PDA) plates, one disc of a 5 mm plug carrying mycelia fungi (P.capsici, F. oxisporum or V. dalhiae) previously grown for 3 days in PDA was placed and each individual bacterium (60I1, 53F, 64S1,42P4) was plated as line. Bacterial strains were cultivated in LB medium for 24 h at 30 °C with orbital shaking (120 rpm). The bacterialcultures were adjusted to a final concentration of 108cells mL−1. The assay was performed incubated at 30 °C for 7 days. The myceliumgrowth was digitally determined every day. A control plate was included growing with the fungi. The percentage of inhibition was calculated comparing to the control. The strains 53F, 64S1 y 42P4 inhibited F. oxisporum and V. dalhiae in vitro mycelium growth.The 42P4 strain inhibited the P. capsici growth in APG plates. Also, we observed the production pyoverdine by 42P4 strain under UV in APG medium.
Fil: Lobato Ureche, Miguel Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ortiz, R.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
BIOCONTROL
IN VITRO
PGPR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154101
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from MendozaLobato Ureche, Miguel AndresPérez Rodriguez, María MicaelaOrtiz, R.Lucero, Gabriela SusanaCohen, Ana CarmenBIOCONTROLIN VITROPGPRhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The rapid growth of the human population and their need of food has impulse to extensive use of chemical fertilizers and pesticides to increase yield crop. These practices are costs and increase environmental pollution. In the last years, appear needs for novel agricultural practices that do not harm natural ecosystems. Different plant growth promotion Rhizobacteria (PGPR) have been studied and incorporated into agricultural practices and biocontrol has emerged in recent years as an alternative to pesticides. PGPR antagonize orprevent the effects of phytopathogens or deleterious microorganisms. PGPR produce substances that protect them against variousdiseases. Metabolites include hydrogen cyanide and antibiotics. Some strain PGPRs produce lytic enzymes as β-1,3-glucanase,chitinase, protease and cellulase that degrade the cell wall of fungi and produce a direct inhibitory effect on the growth of hyphae.Another important mechanism of biocontrol of PGPRs is related to the production of siderophores that chelate iron, making itunavailable to pathogens. In this study four native PGPR strains of Mendoza against pathogenic fungi of pepper crops were evaluated.The test was carried out by fourfold and repeated two times using independent Petri dishes for the growth of each bacterial strain andeach pathogens. On Luria Broth (LB) and potato dextrose agar (PDA) plates, one disc of a 5 mm plug carrying mycelia fungi (P.capsici, F. oxisporum or V. dalhiae) previously grown for 3 days in PDA was placed and each individual bacterium (60I1, 53F, 64S1,42P4) was plated as line. Bacterial strains were cultivated in LB medium for 24 h at 30 °C with orbital shaking (120 rpm). The bacterialcultures were adjusted to a final concentration of 108cells mL−1. The assay was performed incubated at 30 °C for 7 days. The myceliumgrowth was digitally determined every day. A control plate was included growing with the fungi. The percentage of inhibition was calculated comparing to the control. The strains 53F, 64S1 y 42P4 inhibited F. oxisporum and V. dalhiae in vitro mycelium growth.The 42P4 strain inhibited the P. capsici growth in APG plates. Also, we observed the production pyoverdine by 42P4 strain under UV in APG medium.Fil: Lobato Ureche, Miguel Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ortiz, R.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoUniversidad Nacional de San Luis2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154101In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 92-93CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:39Zoai:ri.conicet.gov.ar:11336/154101instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:39.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
title In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
spellingShingle In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
Lobato Ureche, Miguel Andres
BIOCONTROL
IN VITRO
PGPR
title_short In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
title_full In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
title_fullStr In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
title_full_unstemmed In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
title_sort In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza
dc.creator.none.fl_str_mv Lobato Ureche, Miguel Andres
Pérez Rodriguez, María Micaela
Ortiz, R.
Lucero, Gabriela Susana
Cohen, Ana Carmen
author Lobato Ureche, Miguel Andres
author_facet Lobato Ureche, Miguel Andres
Pérez Rodriguez, María Micaela
Ortiz, R.
Lucero, Gabriela Susana
Cohen, Ana Carmen
author_role author
author2 Pérez Rodriguez, María Micaela
Ortiz, R.
Lucero, Gabriela Susana
Cohen, Ana Carmen
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOCONTROL
IN VITRO
PGPR
topic BIOCONTROL
IN VITRO
PGPR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The rapid growth of the human population and their need of food has impulse to extensive use of chemical fertilizers and pesticides to increase yield crop. These practices are costs and increase environmental pollution. In the last years, appear needs for novel agricultural practices that do not harm natural ecosystems. Different plant growth promotion Rhizobacteria (PGPR) have been studied and incorporated into agricultural practices and biocontrol has emerged in recent years as an alternative to pesticides. PGPR antagonize orprevent the effects of phytopathogens or deleterious microorganisms. PGPR produce substances that protect them against variousdiseases. Metabolites include hydrogen cyanide and antibiotics. Some strain PGPRs produce lytic enzymes as β-1,3-glucanase,chitinase, protease and cellulase that degrade the cell wall of fungi and produce a direct inhibitory effect on the growth of hyphae.Another important mechanism of biocontrol of PGPRs is related to the production of siderophores that chelate iron, making itunavailable to pathogens. In this study four native PGPR strains of Mendoza against pathogenic fungi of pepper crops were evaluated.The test was carried out by fourfold and repeated two times using independent Petri dishes for the growth of each bacterial strain andeach pathogens. On Luria Broth (LB) and potato dextrose agar (PDA) plates, one disc of a 5 mm plug carrying mycelia fungi (P.capsici, F. oxisporum or V. dalhiae) previously grown for 3 days in PDA was placed and each individual bacterium (60I1, 53F, 64S1,42P4) was plated as line. Bacterial strains were cultivated in LB medium for 24 h at 30 °C with orbital shaking (120 rpm). The bacterialcultures were adjusted to a final concentration of 108cells mL−1. The assay was performed incubated at 30 °C for 7 days. The myceliumgrowth was digitally determined every day. A control plate was included growing with the fungi. The percentage of inhibition was calculated comparing to the control. The strains 53F, 64S1 y 42P4 inhibited F. oxisporum and V. dalhiae in vitro mycelium growth.The 42P4 strain inhibited the P. capsici growth in APG plates. Also, we observed the production pyoverdine by 42P4 strain under UV in APG medium.
Fil: Lobato Ureche, Miguel Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ortiz, R.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description The rapid growth of the human population and their need of food has impulse to extensive use of chemical fertilizers and pesticides to increase yield crop. These practices are costs and increase environmental pollution. In the last years, appear needs for novel agricultural practices that do not harm natural ecosystems. Different plant growth promotion Rhizobacteria (PGPR) have been studied and incorporated into agricultural practices and biocontrol has emerged in recent years as an alternative to pesticides. PGPR antagonize orprevent the effects of phytopathogens or deleterious microorganisms. PGPR produce substances that protect them against variousdiseases. Metabolites include hydrogen cyanide and antibiotics. Some strain PGPRs produce lytic enzymes as β-1,3-glucanase,chitinase, protease and cellulase that degrade the cell wall of fungi and produce a direct inhibitory effect on the growth of hyphae.Another important mechanism of biocontrol of PGPRs is related to the production of siderophores that chelate iron, making itunavailable to pathogens. In this study four native PGPR strains of Mendoza against pathogenic fungi of pepper crops were evaluated.The test was carried out by fourfold and repeated two times using independent Petri dishes for the growth of each bacterial strain andeach pathogens. On Luria Broth (LB) and potato dextrose agar (PDA) plates, one disc of a 5 mm plug carrying mycelia fungi (P.capsici, F. oxisporum or V. dalhiae) previously grown for 3 days in PDA was placed and each individual bacterium (60I1, 53F, 64S1,42P4) was plated as line. Bacterial strains were cultivated in LB medium for 24 h at 30 °C with orbital shaking (120 rpm). The bacterialcultures were adjusted to a final concentration of 108cells mL−1. The assay was performed incubated at 30 °C for 7 days. The myceliumgrowth was digitally determined every day. A control plate was included growing with the fungi. The percentage of inhibition was calculated comparing to the control. The strains 53F, 64S1 y 42P4 inhibited F. oxisporum and V. dalhiae in vitro mycelium growth.The 42P4 strain inhibited the P. capsici growth in APG plates. Also, we observed the production pyoverdine by 42P4 strain under UV in APG medium.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154101
In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 92-93
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154101
identifier_str_mv In vitro inhibition of Phytophthora capsici, Fusarium oxisporum and Verticillium dalhiae by native plant growth promotion rhizobacteria strains from Mendoza; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 92-93
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de San Luis
publisher.none.fl_str_mv Universidad Nacional de San Luis
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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