Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense

Autores
Viejobueno, Josefina; Albornoz, Patricia Liliana; Camacho, María; de los Santos, Berta; Martinez Zamora, Martin Gustavo; Salazar, Sergio Miguel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replac-ing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Camacho, María. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: de los Santos, Berta. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
Materia
BIOCONTROL
INDUCED RESISTANCE
PGPR
PHYTOPATHOGEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184313
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184313
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilenseViejobueno, JosefinaAlbornoz, Patricia LilianaCamacho, Maríade los Santos, BertaMartinez Zamora, Martin GustavoSalazar, Sergio MiguelBIOCONTROLINDUCED RESISTANCEPGPRPHYTOPATHOGENhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replac-ing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Camacho, María. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; EspañaFil: de los Santos, Berta. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; EspañaFil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; ArgentinaMDPI AG2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184313Viejobueno, Josefina; Albornoz, Patricia Liliana; Camacho, María; de los Santos, Berta; Martinez Zamora, Martin Gustavo; et al.; Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense; MDPI AG; Agronomy; 11; 2; 2-2021; 1-122073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/11/2/195info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy11020195info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:12Zoai:ri.conicet.gov.ar:11336/184313instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:12.494CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
spellingShingle Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
Viejobueno, Josefina
BIOCONTROL
INDUCED RESISTANCE
PGPR
PHYTOPATHOGEN
title_short Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_full Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_fullStr Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_full_unstemmed Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_sort Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
dc.creator.none.fl_str_mv Viejobueno, Josefina
Albornoz, Patricia Liliana
Camacho, María
de los Santos, Berta
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author Viejobueno, Josefina
author_facet Viejobueno, Josefina
Albornoz, Patricia Liliana
Camacho, María
de los Santos, Berta
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author_role author
author2 Albornoz, Patricia Liliana
Camacho, María
de los Santos, Berta
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOCONTROL
INDUCED RESISTANCE
PGPR
PHYTOPATHOGEN
topic BIOCONTROL
INDUCED RESISTANCE
PGPR
PHYTOPATHOGEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replac-ing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Camacho, María. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: de los Santos, Berta. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
description Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replac-ing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184313
Viejobueno, Josefina; Albornoz, Patricia Liliana; Camacho, María; de los Santos, Berta; Martinez Zamora, Martin Gustavo; et al.; Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense; MDPI AG; Agronomy; 11; 2; 2-2021; 1-12
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184313
identifier_str_mv Viejobueno, Josefina; Albornoz, Patricia Liliana; Camacho, María; de los Santos, Berta; Martinez Zamora, Martin Gustavo; et al.; Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense; MDPI AG; Agronomy; 11; 2; 2-2021; 1-12
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/11/2/195
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy11020195
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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