Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers

Autores
Martínez, Ana Laura; Garayalde, Antonio Francisco; Quiroz, Facundo José; Carrera, Alicia Delia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Argentina is the fourth leading producing country of sunflower worldwide. Sunflower downy mildew, caused by the pathogenPlasmopara halstedii, is an economically important disease of sunflower. Since 2012, P. halstedii epiphytotics were identified inseveral production regions of Argentina. However, the genetic diversity of the pathogen in Argentina remains largely unknown. Fortytwofield isolates from Argentina were analyzed using eight loci SSR. The number of alleles per locus (APL), the expected (HE) andobserved heterozygosity (HO) were determined. SSR data were analyzed with regions (two), collection years (four) and races (six) asvariation sources (AMOVA), and the population structure was defined (Bayesian method). DNA sequences of two effector genes wereobtained from two isolates per race and the polymorphisms were analyzed (ClustalW). The mean APL was 2.6, varying from 1 to 4(total 21). The highest number of alleles and races were found in 2017. Average HO and HE were 0 and 0.369, respectively. Geneticvariation was observed among regions (7%) and years (32%), but not between races. Based on effectors, local races were similar to agroup of French races, without variation within the ARG races. Increased genetic variability observed in recent years seems to parallelwith the emergence of novel races reducing fungicides and/or R genes efficacy. The effector study suggests that the races evolutiondepends on country specific factors.
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. North Dakota State University; Estados Unidos
Fil: Garayalde, Antonio Francisco. Universidad Nacional del Sur. Departamento de Matemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
American Phytopathological Society North Central Division Meeting​
Iowa
Estados Unidos
American Phytopathological Society
Materia
plasmopara halstedii
SSR
Genetic characterization
Sunflower
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215475
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215475
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markersMartínez, Ana LauraGarayalde, Antonio FranciscoQuiroz, Facundo JoséCarrera, Alicia Deliaplasmopara halstediiSSRGenetic characterizationSunflowerhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Argentina is the fourth leading producing country of sunflower worldwide. Sunflower downy mildew, caused by the pathogenPlasmopara halstedii, is an economically important disease of sunflower. Since 2012, P. halstedii epiphytotics were identified inseveral production regions of Argentina. However, the genetic diversity of the pathogen in Argentina remains largely unknown. Fortytwofield isolates from Argentina were analyzed using eight loci SSR. The number of alleles per locus (APL), the expected (HE) andobserved heterozygosity (HO) were determined. SSR data were analyzed with regions (two), collection years (four) and races (six) asvariation sources (AMOVA), and the population structure was defined (Bayesian method). DNA sequences of two effector genes wereobtained from two isolates per race and the polymorphisms were analyzed (ClustalW). The mean APL was 2.6, varying from 1 to 4(total 21). The highest number of alleles and races were found in 2017. Average HO and HE were 0 and 0.369, respectively. Geneticvariation was observed among regions (7%) and years (32%), but not between races. Based on effectors, local races were similar to agroup of French races, without variation within the ARG races. Increased genetic variability observed in recent years seems to parallelwith the emergence of novel races reducing fungicides and/or R genes efficacy. The effector study suggests that the races evolutiondepends on country specific factors.Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. North Dakota State University; Estados UnidosFil: Garayalde, Antonio Francisco. Universidad Nacional del Sur. Departamento de Matemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaAmerican Phytopathological Society North Central Division Meeting​IowaEstados UnidosAmerican Phytopathological SocietyThe American Phytopathological Society2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215475Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers; American Phytopathological Society North Central Division Meeting​; Iowa; Estados Unidos; 2021; S1.27-S1.270031-949X1943-7684CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-111-9-S1.26info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-111-9-S1.26Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:50Zoai:ri.conicet.gov.ar:11336/215475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:50.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
title Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
spellingShingle Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
Martínez, Ana Laura
plasmopara halstedii
SSR
Genetic characterization
Sunflower
title_short Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
title_full Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
title_fullStr Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
title_full_unstemmed Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
title_sort Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers
dc.creator.none.fl_str_mv Martínez, Ana Laura
Garayalde, Antonio Francisco
Quiroz, Facundo José
Carrera, Alicia Delia
author Martínez, Ana Laura
author_facet Martínez, Ana Laura
Garayalde, Antonio Francisco
Quiroz, Facundo José
Carrera, Alicia Delia
author_role author
author2 Garayalde, Antonio Francisco
Quiroz, Facundo José
Carrera, Alicia Delia
author2_role author
author
author
dc.subject.none.fl_str_mv plasmopara halstedii
SSR
Genetic characterization
Sunflower
topic plasmopara halstedii
SSR
Genetic characterization
Sunflower
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Argentina is the fourth leading producing country of sunflower worldwide. Sunflower downy mildew, caused by the pathogenPlasmopara halstedii, is an economically important disease of sunflower. Since 2012, P. halstedii epiphytotics were identified inseveral production regions of Argentina. However, the genetic diversity of the pathogen in Argentina remains largely unknown. Fortytwofield isolates from Argentina were analyzed using eight loci SSR. The number of alleles per locus (APL), the expected (HE) andobserved heterozygosity (HO) were determined. SSR data were analyzed with regions (two), collection years (four) and races (six) asvariation sources (AMOVA), and the population structure was defined (Bayesian method). DNA sequences of two effector genes wereobtained from two isolates per race and the polymorphisms were analyzed (ClustalW). The mean APL was 2.6, varying from 1 to 4(total 21). The highest number of alleles and races were found in 2017. Average HO and HE were 0 and 0.369, respectively. Geneticvariation was observed among regions (7%) and years (32%), but not between races. Based on effectors, local races were similar to agroup of French races, without variation within the ARG races. Increased genetic variability observed in recent years seems to parallelwith the emergence of novel races reducing fungicides and/or R genes efficacy. The effector study suggests that the races evolutiondepends on country specific factors.
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. North Dakota State University; Estados Unidos
Fil: Garayalde, Antonio Francisco. Universidad Nacional del Sur. Departamento de Matemática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
American Phytopathological Society North Central Division Meeting​
Iowa
Estados Unidos
American Phytopathological Society
description Argentina is the fourth leading producing country of sunflower worldwide. Sunflower downy mildew, caused by the pathogenPlasmopara halstedii, is an economically important disease of sunflower. Since 2012, P. halstedii epiphytotics were identified inseveral production regions of Argentina. However, the genetic diversity of the pathogen in Argentina remains largely unknown. Fortytwofield isolates from Argentina were analyzed using eight loci SSR. The number of alleles per locus (APL), the expected (HE) andobserved heterozygosity (HO) were determined. SSR data were analyzed with regions (two), collection years (four) and races (six) asvariation sources (AMOVA), and the population structure was defined (Bayesian method). DNA sequences of two effector genes wereobtained from two isolates per race and the polymorphisms were analyzed (ClustalW). The mean APL was 2.6, varying from 1 to 4(total 21). The highest number of alleles and races were found in 2017. Average HO and HE were 0 and 0.369, respectively. Geneticvariation was observed among regions (7%) and years (32%), but not between races. Based on effectors, local races were similar to agroup of French races, without variation within the ARG races. Increased genetic variability observed in recent years seems to parallelwith the emergence of novel races reducing fungicides and/or R genes efficacy. The effector study suggests that the races evolutiondepends on country specific factors.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215475
Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers; American Phytopathological Society North Central Division Meeting​; Iowa; Estados Unidos; 2021; S1.27-S1.27
0031-949X
1943-7684
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215475
identifier_str_mv Genetic characterization of Plasmopara halstedii populations in Argentina using simple sequence repeats (SSR) and effectorbased markers; American Phytopathological Society North Central Division Meeting​; Iowa; Estados Unidos; 2021; S1.27-S1.27
0031-949X
1943-7684
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-111-9-S1.26
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-111-9-S1.26
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv The American Phytopathological Society
publisher.none.fl_str_mv The American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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