Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.

Autores
Martínez, Ana Laura; Quiroz, Facundo Jose; Carrera, Alicia Delia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
EEA Balcarce
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina.
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina.
Fuente
Journal of the Saudi Society of Agricultural Sciences (Available online 6 May 2021)
Materia
Helianthus Annuus
Semilla de Girasol
Plasmopara Halstedii
Mildiu
PCR
Marcadores Genéticos
Enfermedades Fungosas
Sunflower Seed
Genetic Markers
Fungal Diseases
Girasol
Sunflower
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10141
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10141
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.Martínez, Ana LauraQuiroz, Facundo JoseCarrera, Alicia DeliaHelianthus AnnuusSemilla de GirasolPlasmopara HalstediiMildiuPCRMarcadores GenéticosEnfermedades FungosasSunflower SeedGenetic MarkersFungal DiseasesGirasolSunflowerPlasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.EEA BalcarceFil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina.Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina.Elsevier2021-08-30T13:13:36Z2021-08-30T13:13:36Z2021-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10141https://www.sciencedirect.com/science/article/pii/S1658077X210005031658-077Xhttps://doi.org/10.1016/j.jssas.2021.04.007Journal of the Saudi Society of Agricultural Sciences (Available online 6 May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:03Zoai:localhost:20.500.12123/10141instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:03.775INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
title Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
spellingShingle Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
Martínez, Ana Laura
Helianthus Annuus
Semilla de Girasol
Plasmopara Halstedii
Mildiu
PCR
Marcadores Genéticos
Enfermedades Fungosas
Sunflower Seed
Genetic Markers
Fungal Diseases
Girasol
Sunflower
title_short Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
title_full Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
title_fullStr Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
title_full_unstemmed Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
title_sort Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
dc.creator.none.fl_str_mv Martínez, Ana Laura
Quiroz, Facundo Jose
Carrera, Alicia Delia
author Martínez, Ana Laura
author_facet Martínez, Ana Laura
Quiroz, Facundo Jose
Carrera, Alicia Delia
author_role author
author2 Quiroz, Facundo Jose
Carrera, Alicia Delia
author2_role author
author
dc.subject.none.fl_str_mv Helianthus Annuus
Semilla de Girasol
Plasmopara Halstedii
Mildiu
PCR
Marcadores Genéticos
Enfermedades Fungosas
Sunflower Seed
Genetic Markers
Fungal Diseases
Girasol
Sunflower
topic Helianthus Annuus
Semilla de Girasol
Plasmopara Halstedii
Mildiu
PCR
Marcadores Genéticos
Enfermedades Fungosas
Sunflower Seed
Genetic Markers
Fungal Diseases
Girasol
Sunflower
dc.description.none.fl_txt_mv Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
EEA Balcarce
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina.
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina.
description Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-30T13:13:36Z
2021-08-30T13:13:36Z
2021-05-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10141
https://www.sciencedirect.com/science/article/pii/S1658077X21000503
1658-077X
https://doi.org/10.1016/j.jssas.2021.04.007
url http://hdl.handle.net/20.500.12123/10141
https://www.sciencedirect.com/science/article/pii/S1658077X21000503
https://doi.org/10.1016/j.jssas.2021.04.007
identifier_str_mv 1658-077X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of the Saudi Society of Agricultural Sciences (Available online 6 May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341390220001280
score 12.623145

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