Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing

Autores
Martínez, Ana Laura; Quiroz, Facundo José; Carrera, Alicia Delia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen´s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Materia
EFFECTOR MARKER
HELIANTHUS ANNUUS
PCR
PLASMOPARA HALSTEDII
SUNFLOWER DOWNY MILDEW
SUNFLOWER SEEDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150651
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/150651
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testingMartínez, Ana LauraQuiroz, Facundo JoséCarrera, Alicia DeliaEFFECTOR MARKERHELIANTHUS ANNUUSPCRPLASMOPARA HALSTEDIISUNFLOWER DOWNY MILDEWSUNFLOWER SEEDShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen´s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; ArgentinaFil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaKing Saud University2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150651Martínez, Ana Laura; Quiroz, Facundo José; Carrera, Alicia Delia; Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing; King Saud University; Journal of the Saudi Society of Agricultural Sciences; 20; 6; 9-2021; 395-4001658-077XCONICET DigitalCONICETenghttps://doi.org/10.1016/j.jssas.2021.10.006info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jssas.2021.04.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1658077X21000503info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:59Zoai:ri.conicet.gov.ar:11336/150651instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:00.17CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
title Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
spellingShingle Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
Martínez, Ana Laura
EFFECTOR MARKER
HELIANTHUS ANNUUS
PCR
PLASMOPARA HALSTEDII
SUNFLOWER DOWNY MILDEW
SUNFLOWER SEEDS
title_short Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
title_full Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
title_fullStr Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
title_full_unstemmed Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
title_sort Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
dc.creator.none.fl_str_mv Martínez, Ana Laura
Quiroz, Facundo José
Carrera, Alicia Delia
author Martínez, Ana Laura
author_facet Martínez, Ana Laura
Quiroz, Facundo José
Carrera, Alicia Delia
author_role author
author2 Quiroz, Facundo José
Carrera, Alicia Delia
author2_role author
author
dc.subject.none.fl_str_mv EFFECTOR MARKER
HELIANTHUS ANNUUS
PCR
PLASMOPARA HALSTEDII
SUNFLOWER DOWNY MILDEW
SUNFLOWER SEEDS
topic EFFECTOR MARKER
HELIANTHUS ANNUUS
PCR
PLASMOPARA HALSTEDII
SUNFLOWER DOWNY MILDEW
SUNFLOWER SEEDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen´s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
description Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen´s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150651
Martínez, Ana Laura; Quiroz, Facundo José; Carrera, Alicia Delia; Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing; King Saud University; Journal of the Saudi Society of Agricultural Sciences; 20; 6; 9-2021; 395-400
1658-077X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150651
identifier_str_mv Martínez, Ana Laura; Quiroz, Facundo José; Carrera, Alicia Delia; Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing; King Saud University; Journal of the Saudi Society of Agricultural Sciences; 20; 6; 9-2021; 395-400
1658-077X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.jssas.2021.10.006
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jssas.2021.04.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1658077X21000503
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv King Saud University
publisher.none.fl_str_mv King Saud University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268948402274304
score 13.13397

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