Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry
- Autores
- Bertani, Milena Sabrina; Lopez Rizo, Maria Carolina; Quiroga, Maria; Garro, Marisa Selva; Perez Chaia, Adriana Beatriz
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The use of probiotics in animal production offers a natural alternative to antibiotic suplementation as growth promoters. The efficiency of probiotics depends on the production and preservation technologies applied as well as on their ability to persist in the gastrointestinal tract. The adhesion to intestinal epithelial cells (IEC) plays a vital role in their persistence, but it may be affected by factors that modify superficial structures. Thus, the aim of this work was to study the development of probiotic poultry strains on different culture media and assess the influence of such media components on their capacity to adhere to IEC of BB chicks. Towards this end, probiotic strains Enterococcus faecium LET301, Lactobacillus salivarius LET201 and L. reuteri LET210 were grown at 37ºC in both conventional culture media and a specifically designed medium containing local industries by-products (yeast cream, cane molasse, whey), and adjusted to 1 x 108 CFU/ml. IEC were obtained from ileum of fourteen-day-old broilers and their concentration was adjusted to 5 x 105cells/ml. The IEC were incubated with the bacterial suspensions and the adhesion percentage and index were determined by microscopy. E. faecium LET301 and L. reuteri LET210 showed higher adhesion to IEC than L. salivarius LET201 in all the studied conditions. These results demonstrate that the evaluated by-products did not significantly affect the adhesion of the probiotic strains. Thus, this medium can be successfully used as an economic alternative for the development of poultry probiotics.
Fil: Bertani, Milena Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lopez Rizo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Quiroga, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; Argentina
Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXXIV Jornadas Científicas Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán - Materia
-
MEDIO DE CULTIVO
PROBIOTICOS
AVES DE CORRAL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/215767
Ver los metadatos del registro completo
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Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultryBertani, Milena SabrinaLopez Rizo, Maria CarolinaQuiroga, MariaGarro, Marisa SelvaPerez Chaia, Adriana BeatrizMEDIO DE CULTIVOPROBIOTICOSAVES DE CORRALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of probiotics in animal production offers a natural alternative to antibiotic suplementation as growth promoters. The efficiency of probiotics depends on the production and preservation technologies applied as well as on their ability to persist in the gastrointestinal tract. The adhesion to intestinal epithelial cells (IEC) plays a vital role in their persistence, but it may be affected by factors that modify superficial structures. Thus, the aim of this work was to study the development of probiotic poultry strains on different culture media and assess the influence of such media components on their capacity to adhere to IEC of BB chicks. Towards this end, probiotic strains Enterococcus faecium LET301, Lactobacillus salivarius LET201 and L. reuteri LET210 were grown at 37ºC in both conventional culture media and a specifically designed medium containing local industries by-products (yeast cream, cane molasse, whey), and adjusted to 1 x 108 CFU/ml. IEC were obtained from ileum of fourteen-day-old broilers and their concentration was adjusted to 5 x 105cells/ml. The IEC were incubated with the bacterial suspensions and the adhesion percentage and index were determined by microscopy. E. faecium LET301 and L. reuteri LET210 showed higher adhesion to IEC than L. salivarius LET201 in all the studied conditions. These results demonstrate that the evaluated by-products did not significantly affect the adhesion of the probiotic strains. Thus, this medium can be successfully used as an economic alternative for the development of poultry probiotics.Fil: Bertani, Milena Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Lopez Rizo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Quiroga, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; ArgentinaFil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXXXIV Jornadas Científicas Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215767Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry; XXXIV Jornadas Científicas Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2017; 13-130327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1528205754_QmlvY2VsbCAyMDE3LnBkZg==.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:31Zoai:ri.conicet.gov.ar:11336/215767instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:32.154CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| title |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| spellingShingle |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry Bertani, Milena Sabrina MEDIO DE CULTIVO PROBIOTICOS AVES DE CORRAL |
| title_short |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| title_full |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| title_fullStr |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| title_full_unstemmed |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| title_sort |
Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry |
| dc.creator.none.fl_str_mv |
Bertani, Milena Sabrina Lopez Rizo, Maria Carolina Quiroga, Maria Garro, Marisa Selva Perez Chaia, Adriana Beatriz |
| author |
Bertani, Milena Sabrina |
| author_facet |
Bertani, Milena Sabrina Lopez Rizo, Maria Carolina Quiroga, Maria Garro, Marisa Selva Perez Chaia, Adriana Beatriz |
| author_role |
author |
| author2 |
Lopez Rizo, Maria Carolina Quiroga, Maria Garro, Marisa Selva Perez Chaia, Adriana Beatriz |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
MEDIO DE CULTIVO PROBIOTICOS AVES DE CORRAL |
| topic |
MEDIO DE CULTIVO PROBIOTICOS AVES DE CORRAL |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The use of probiotics in animal production offers a natural alternative to antibiotic suplementation as growth promoters. The efficiency of probiotics depends on the production and preservation technologies applied as well as on their ability to persist in the gastrointestinal tract. The adhesion to intestinal epithelial cells (IEC) plays a vital role in their persistence, but it may be affected by factors that modify superficial structures. Thus, the aim of this work was to study the development of probiotic poultry strains on different culture media and assess the influence of such media components on their capacity to adhere to IEC of BB chicks. Towards this end, probiotic strains Enterococcus faecium LET301, Lactobacillus salivarius LET201 and L. reuteri LET210 were grown at 37ºC in both conventional culture media and a specifically designed medium containing local industries by-products (yeast cream, cane molasse, whey), and adjusted to 1 x 108 CFU/ml. IEC were obtained from ileum of fourteen-day-old broilers and their concentration was adjusted to 5 x 105cells/ml. The IEC were incubated with the bacterial suspensions and the adhesion percentage and index were determined by microscopy. E. faecium LET301 and L. reuteri LET210 showed higher adhesion to IEC than L. salivarius LET201 in all the studied conditions. These results demonstrate that the evaluated by-products did not significantly affect the adhesion of the probiotic strains. Thus, this medium can be successfully used as an economic alternative for the development of poultry probiotics. Fil: Bertani, Milena Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Lopez Rizo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Quiroga, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Microbiología General; Argentina Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina XXXIV Jornadas Científicas Asociación de Biología de Tucumán Tafí del Valle Argentina Asociación de Biología de Tucumán |
| description |
The use of probiotics in animal production offers a natural alternative to antibiotic suplementation as growth promoters. The efficiency of probiotics depends on the production and preservation technologies applied as well as on their ability to persist in the gastrointestinal tract. The adhesion to intestinal epithelial cells (IEC) plays a vital role in their persistence, but it may be affected by factors that modify superficial structures. Thus, the aim of this work was to study the development of probiotic poultry strains on different culture media and assess the influence of such media components on their capacity to adhere to IEC of BB chicks. Towards this end, probiotic strains Enterococcus faecium LET301, Lactobacillus salivarius LET201 and L. reuteri LET210 were grown at 37ºC in both conventional culture media and a specifically designed medium containing local industries by-products (yeast cream, cane molasse, whey), and adjusted to 1 x 108 CFU/ml. IEC were obtained from ileum of fourteen-day-old broilers and their concentration was adjusted to 5 x 105cells/ml. The IEC were incubated with the bacterial suspensions and the adhesion percentage and index were determined by microscopy. E. faecium LET301 and L. reuteri LET210 showed higher adhesion to IEC than L. salivarius LET201 in all the studied conditions. These results demonstrate that the evaluated by-products did not significantly affect the adhesion of the probiotic strains. Thus, this medium can be successfully used as an economic alternative for the development of poultry probiotics. |
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2018 |
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2018 |
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http://hdl.handle.net/11336/215767 Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry; XXXIV Jornadas Científicas Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2017; 13-13 0327-9545 1667-5746 CONICET Digital CONICET |
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Effect of the composition of probiotic bacteria culture medium on their capacity to adhere to intestinal epithelial cells of poultry; XXXIV Jornadas Científicas Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2017; 13-13 0327-9545 1667-5746 CONICET Digital CONICET |
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eng |
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