Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs

Autores
Pizzolitto, Romina P.; Armando, María R.; Combina, Mariana; Cavaglieri, Lilia Reneé; Dalcero, Ana María; Salvano, Mario A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host.
Fil: Pizzolitto, Romina P. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Armando, María R. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Salvano, Mario A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
Fuente
Journal of environmental science and health. Part B 47 (10) : 933-941. (2012)
Materia
Saccharomyces Cerevisiae
Aflatoxinas
Aves de Corral
Piensos
Aflatoxins
Poultry
Feeds
Probiotics
Probióticos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1694
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1694
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spelling Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffsPizzolitto, Romina P.Armando, María R.Combina, MarianaCavaglieri, Lilia ReneéDalcero, Ana MaríaSalvano, Mario A.Saccharomyces CerevisiaeAflatoxinasAves de CorralPiensosAflatoxinsPoultryFeedsProbioticsProbióticosIn this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host.Fil: Pizzolitto, Romina P. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Armando, María R. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Dalcero, Ana María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Salvano, Mario A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina2017-11-07T13:53:06Z2017-11-07T13:53:06Z2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1694http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb200360-1234 (Print)1532-4109 (Online)http://dx.doi.org/10.1080/03601234.2012.706558Journal of environmental science and health. Part B 47 (10) : 933-941. (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:13Zoai:localhost:20.500.12123/1694instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:14.054INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
title Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
spellingShingle Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
Pizzolitto, Romina P.
Saccharomyces Cerevisiae
Aflatoxinas
Aves de Corral
Piensos
Aflatoxins
Poultry
Feeds
Probiotics
Probióticos
title_short Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
title_full Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
title_fullStr Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
title_full_unstemmed Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
title_sort Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
dc.creator.none.fl_str_mv Pizzolitto, Romina P.
Armando, María R.
Combina, Mariana
Cavaglieri, Lilia Reneé
Dalcero, Ana María
Salvano, Mario A.
author Pizzolitto, Romina P.
author_facet Pizzolitto, Romina P.
Armando, María R.
Combina, Mariana
Cavaglieri, Lilia Reneé
Dalcero, Ana María
Salvano, Mario A.
author_role author
author2 Armando, María R.
Combina, Mariana
Cavaglieri, Lilia Reneé
Dalcero, Ana María
Salvano, Mario A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Saccharomyces Cerevisiae
Aflatoxinas
Aves de Corral
Piensos
Aflatoxins
Poultry
Feeds
Probiotics
Probióticos
topic Saccharomyces Cerevisiae
Aflatoxinas
Aves de Corral
Piensos
Aflatoxins
Poultry
Feeds
Probiotics
Probióticos
dc.description.none.fl_txt_mv In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host.
Fil: Pizzolitto, Romina P. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Armando, María R. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Salvano, Mario A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina
description In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
2017-11-07T13:53:06Z
2017-11-07T13:53:06Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1694
http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb20
0360-1234 (Print)
1532-4109 (Online)
http://dx.doi.org/10.1080/03601234.2012.706558
url http://hdl.handle.net/20.500.12123/1694
http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb20
http://dx.doi.org/10.1080/03601234.2012.706558
identifier_str_mv 0360-1234 (Print)
1532-4109 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of environmental science and health. Part B 47 (10) : 933-941. (2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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