Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery

Autores
Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; Romanus, Omeh; Nnabuike Didacus, Nnamani; Chimaobi Kenechukwu, Franklin; Audu Mumuni, Momoh; Saldías, César; Díaz Díaz, David
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.
Fil: Chibuzor Amadi, Ben. University of Nigeria; Nigeria
Fil: Akpa, Paul. University of Nigeria; Nigeria
Fil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Alfa, John. Bingham University Karu; Nigeria
Fil: Romanus, Omeh. Enugu State University of Science and Technology; Nigeria
Fil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; Nigeria
Fil: Chimaobi Kenechukwu, Franklin. University of Nigeria; Nigeria
Fil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; Nigeria
Fil: Saldías, César. Pontificia Universidad Católica de Chile; Chile
Fil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; España
Materia
INSULIN
SODIUM-ALGUINATE
DIABETES
GELATIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262308
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262308
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin DeliveryChibuzor Amadi, BenAkpa, PaulBuxaderas, EduardoMoglie, Yanina FernandaAlfa, JohnRomanus, OmehNnabuike Didacus, NnamaniChimaobi Kenechukwu, FranklinAudu Mumuni, MomohSaldías, CésarDíaz Díaz, DavidINSULINSODIUM-ALGUINATEDIABETESGELATINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.Fil: Chibuzor Amadi, Ben. University of Nigeria; NigeriaFil: Akpa, Paul. University of Nigeria; NigeriaFil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Alfa, John. Bingham University Karu; NigeriaFil: Romanus, Omeh. Enugu State University of Science and Technology; NigeriaFil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; NigeriaFil: Chimaobi Kenechukwu, Franklin. University of Nigeria; NigeriaFil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; NigeriaFil: Saldías, César. Pontificia Universidad Católica de Chile; ChileFil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; EspañaUniversidad de Nigeria. Facultad de Ciencias Farmacéuticas2024-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262308Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-1160794-800X1596-2431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ajopred.com/development-of-gelatin-sodium-alginate-microparticles-for-oral-insulin-delivery/info:eu-repo/semantics/altIdentifier/doi/10.59493/ajopred/2024.3.12info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:21Zoai:ri.conicet.gov.ar:11336/262308instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:21.426CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
title Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
spellingShingle Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
Chibuzor Amadi, Ben
INSULIN
SODIUM-ALGUINATE
DIABETES
GELATIN
title_short Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
title_full Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
title_fullStr Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
title_full_unstemmed Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
title_sort Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
dc.creator.none.fl_str_mv Chibuzor Amadi, Ben
Akpa, Paul
Buxaderas, Eduardo
Moglie, Yanina Fernanda
Alfa, John
Romanus, Omeh
Nnabuike Didacus, Nnamani
Chimaobi Kenechukwu, Franklin
Audu Mumuni, Momoh
Saldías, César
Díaz Díaz, David
author Chibuzor Amadi, Ben
author_facet Chibuzor Amadi, Ben
Akpa, Paul
Buxaderas, Eduardo
Moglie, Yanina Fernanda
Alfa, John
Romanus, Omeh
Nnabuike Didacus, Nnamani
Chimaobi Kenechukwu, Franklin
Audu Mumuni, Momoh
Saldías, César
Díaz Díaz, David
author_role author
author2 Akpa, Paul
Buxaderas, Eduardo
Moglie, Yanina Fernanda
Alfa, John
Romanus, Omeh
Nnabuike Didacus, Nnamani
Chimaobi Kenechukwu, Franklin
Audu Mumuni, Momoh
Saldías, César
Díaz Díaz, David
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INSULIN
SODIUM-ALGUINATE
DIABETES
GELATIN
topic INSULIN
SODIUM-ALGUINATE
DIABETES
GELATIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.
Fil: Chibuzor Amadi, Ben. University of Nigeria; Nigeria
Fil: Akpa, Paul. University of Nigeria; Nigeria
Fil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Alfa, John. Bingham University Karu; Nigeria
Fil: Romanus, Omeh. Enugu State University of Science and Technology; Nigeria
Fil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; Nigeria
Fil: Chimaobi Kenechukwu, Franklin. University of Nigeria; Nigeria
Fil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; Nigeria
Fil: Saldías, César. Pontificia Universidad Católica de Chile; Chile
Fil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; España
description Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262308
Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-116
0794-800X
1596-2431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262308
identifier_str_mv Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-116
0794-800X
1596-2431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ajopred.com/development-of-gelatin-sodium-alginate-microparticles-for-oral-insulin-delivery/
info:eu-repo/semantics/altIdentifier/doi/10.59493/ajopred/2024.3.12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad de Nigeria. Facultad de Ciencias Farmacéuticas
publisher.none.fl_str_mv Universidad de Nigeria. Facultad de Ciencias Farmacéuticas
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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