Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery
- Autores
- Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; Romanus, Omeh; Nnabuike Didacus, Nnamani; Chimaobi Kenechukwu, Franklin; Audu Mumuni, Momoh; Saldías, César; Díaz Díaz, David
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.
Fil: Chibuzor Amadi, Ben. University of Nigeria; Nigeria
Fil: Akpa, Paul. University of Nigeria; Nigeria
Fil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Alfa, John. Bingham University Karu; Nigeria
Fil: Romanus, Omeh. Enugu State University of Science and Technology; Nigeria
Fil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; Nigeria
Fil: Chimaobi Kenechukwu, Franklin. University of Nigeria; Nigeria
Fil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; Nigeria
Fil: Saldías, César. Pontificia Universidad Católica de Chile; Chile
Fil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; España - Materia
-
INSULIN
SODIUM-ALGUINATE
DIABETES
GELATIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/262308
Ver los metadatos del registro completo
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Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin DeliveryChibuzor Amadi, BenAkpa, PaulBuxaderas, EduardoMoglie, Yanina FernandaAlfa, JohnRomanus, OmehNnabuike Didacus, NnamaniChimaobi Kenechukwu, FranklinAudu Mumuni, MomohSaldías, CésarDíaz Díaz, DavidINSULINSODIUM-ALGUINATEDIABETESGELATINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.Fil: Chibuzor Amadi, Ben. University of Nigeria; NigeriaFil: Akpa, Paul. University of Nigeria; NigeriaFil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Alfa, John. Bingham University Karu; NigeriaFil: Romanus, Omeh. Enugu State University of Science and Technology; NigeriaFil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; NigeriaFil: Chimaobi Kenechukwu, Franklin. University of Nigeria; NigeriaFil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; NigeriaFil: Saldías, César. Pontificia Universidad Católica de Chile; ChileFil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; EspañaUniversidad de Nigeria. Facultad de Ciencias Farmacéuticas2024-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262308Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-1160794-800X1596-2431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ajopred.com/development-of-gelatin-sodium-alginate-microparticles-for-oral-insulin-delivery/info:eu-repo/semantics/altIdentifier/doi/10.59493/ajopred/2024.3.12info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:21Zoai:ri.conicet.gov.ar:11336/262308instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:21.426CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
title |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
spellingShingle |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery Chibuzor Amadi, Ben INSULIN SODIUM-ALGUINATE DIABETES GELATIN |
title_short |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
title_full |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
title_fullStr |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
title_full_unstemmed |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
title_sort |
Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery |
dc.creator.none.fl_str_mv |
Chibuzor Amadi, Ben Akpa, Paul Buxaderas, Eduardo Moglie, Yanina Fernanda Alfa, John Romanus, Omeh Nnabuike Didacus, Nnamani Chimaobi Kenechukwu, Franklin Audu Mumuni, Momoh Saldías, César Díaz Díaz, David |
author |
Chibuzor Amadi, Ben |
author_facet |
Chibuzor Amadi, Ben Akpa, Paul Buxaderas, Eduardo Moglie, Yanina Fernanda Alfa, John Romanus, Omeh Nnabuike Didacus, Nnamani Chimaobi Kenechukwu, Franklin Audu Mumuni, Momoh Saldías, César Díaz Díaz, David |
author_role |
author |
author2 |
Akpa, Paul Buxaderas, Eduardo Moglie, Yanina Fernanda Alfa, John Romanus, Omeh Nnabuike Didacus, Nnamani Chimaobi Kenechukwu, Franklin Audu Mumuni, Momoh Saldías, César Díaz Díaz, David |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
INSULIN SODIUM-ALGUINATE DIABETES GELATIN |
topic |
INSULIN SODIUM-ALGUINATE DIABETES GELATIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin. Fil: Chibuzor Amadi, Ben. University of Nigeria; Nigeria Fil: Akpa, Paul. University of Nigeria; Nigeria Fil: Buxaderas, Eduardo. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina Fil: Moglie, Yanina Fernanda. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina Fil: Alfa, John. Bingham University Karu; Nigeria Fil: Romanus, Omeh. Enugu State University of Science and Technology; Nigeria Fil: Nnabuike Didacus, Nnamani. Igbinedion University Okada; Nigeria Fil: Chimaobi Kenechukwu, Franklin. University of Nigeria; Nigeria Fil: Audu Mumuni, Momoh. University of Nigeria; Nigeria. Bingham University Karu; Nigeria Fil: Saldías, César. Pontificia Universidad Católica de Chile; Chile Fil: Díaz Díaz, David. Universidad de la Laguna. Departamento de Química Orgánica. Instituto Universitario de Bio-Orgánica "Antonio González"; España. Universidad de La Laguna; España |
description |
Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed insubcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-12-20 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/262308 Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-116 0794-800X 1596-2431 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/262308 |
identifier_str_mv |
Chibuzor Amadi, Ben; Akpa, Paul; Buxaderas, Eduardo; Moglie, Yanina Fernanda; Alfa, John; et al.; Development of Gelatin-Sodium Alginate Microparticles for Oral Insulin Delivery; Universidad de Nigeria. Facultad de Ciencias Farmacéuticas; The African Journal of Pharmaceutical Research and Development; 16; 3; 20-12-2024; 105-116 0794-800X 1596-2431 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://ajopred.com/development-of-gelatin-sodium-alginate-microparticles-for-oral-insulin-delivery/ info:eu-repo/semantics/altIdentifier/doi/10.59493/ajopred/2024.3.12 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidad de Nigeria. Facultad de Ciencias Farmacéuticas |
publisher.none.fl_str_mv |
Universidad de Nigeria. Facultad de Ciencias Farmacéuticas |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083176166850560 |
score |
13.22299 |