Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

Autores
Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; Hertz, Plinho Francisco
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.
Fil: Hackenhaar, Camila Regina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Flores Rosa, Carolina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Esparza Flores, Elí Emanuel. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Poletto Klein, Manuela. Universidade Federal de Ciencias Da Saúde de Porto Alegre; Brasil
Fil: Hertz, Plinho Francisco. Universidade Federal do Rio Grande do Sul; Brasil
Materia
ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/209624
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/209624
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristicsHackenhaar, Camila ReginaFlores Rosa, CarolinaEsparza Flores, Elí EmanuelSantagapita, Patricio RomanPoletto Klein, ManuelaHertz, Plinho FranciscoASPERGILLUS ORYZAEGELATINGENIPINIMMOBILIZATIONLACTOSE HYDROLYSISSODIUM ALGINATEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.Fil: Hackenhaar, Camila Regina. Universidade Federal do Rio Grande do Sul; BrasilFil: Flores Rosa, Carolina. Universidade Federal do Rio Grande do Sul; BrasilFil: Esparza Flores, Elí Emanuel. Universidade Federal do Rio Grande do Sul; BrasilFil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Poletto Klein, Manuela. Universidade Federal de Ciencias Da Saúde de Porto Alegre; BrasilFil: Hertz, Plinho Francisco. Universidade Federal do Rio Grande do Sul; BrasilElsevier2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/209624Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-370144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722003885info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.119483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:44Zoai:ri.conicet.gov.ar:11336/209624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:44.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
spellingShingle Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
Hackenhaar, Camila Regina
ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
title_short Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_full Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_fullStr Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_full_unstemmed Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_sort Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
dc.creator.none.fl_str_mv Hackenhaar, Camila Regina
Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author Hackenhaar, Camila Regina
author_facet Hackenhaar, Camila Regina
Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author_role author
author2 Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
topic ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.
Fil: Hackenhaar, Camila Regina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Flores Rosa, Carolina. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Esparza Flores, Elí Emanuel. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Poletto Klein, Manuela. Universidade Federal de Ciencias Da Saúde de Porto Alegre; Brasil
Fil: Hertz, Plinho Francisco. Universidade Federal do Rio Grande do Sul; Brasil
description In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/209624
Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-37
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/209624
identifier_str_mv Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-37
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722003885
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.119483
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613979037499392
score 13.070432

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