Heat stress affects the metabolism of lipids of epididymal epithelial cells

Autores
Luquez, Jessica Mariela; Oresti, Gerardo Martin; Valles, Ana Sofia; Aveldaño, Marta Isabel; Furland, Natalia Edith
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Frequent exposures of testes to hyperthermia are known to result in germ cell death, and, although not lethal to Sertoli cells, we previously showed that such exposures damage them structurally and functionally. Here we studied the consequences of heat stress on the epididymis by assessing its effects on epithelial cells from its three regions (caput, corpus, and cauda) in primary culture. All of them had abundant lipid droplets (LD) rich in triacylglycerols (TAG), similar in size and numbers in vivo and in culture. Temperature-treated (a 15 min exposure once a day to 43ºC for 5 days) and control cells were incubated for 1 h with [3H]20:4 on the 5th day. In the three regions, TAG were faintly labeled, most of the fatty acid being taken up by phospholipids. Caput-derived cells exhibited the highest rate of lipid labeling. Due to heat exposures, caput and corpus cells showed a significant increase in the label from [3H]20:4 in diacylglycerols (DAG), concomitant with a decrease in the label in phosphatidylinositol (PI), phospatidylethanolamine phosphatidylglycerol (PE-PG), and little change in phosphatidylcholine (PC). Quite the opposite occurred in cells from cauda: the label in DAG decreased, while that in PI, PE-PG and PC increased. Our results suggest that epididymal cells are differentially susceptible to short but periodical exposures to mild hyperthermia.
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
L Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
HIPERTERMIA
CÉLULAS EPIDIDIMALES
SEGMENTOS EPIDIDIMALES
FOSFOLÍPIDOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234467
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/234467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heat stress affects the metabolism of lipids of epididymal epithelial cellsLuquez, Jessica MarielaOresti, Gerardo MartinValles, Ana SofiaAveldaño, Marta IsabelFurland, Natalia EdithHIPERTERMIACÉLULAS EPIDIDIMALESSEGMENTOS EPIDIDIMALESFOSFOLÍPIDOShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Frequent exposures of testes to hyperthermia are known to result in germ cell death, and, although not lethal to Sertoli cells, we previously showed that such exposures damage them structurally and functionally. Here we studied the consequences of heat stress on the epididymis by assessing its effects on epithelial cells from its three regions (caput, corpus, and cauda) in primary culture. All of them had abundant lipid droplets (LD) rich in triacylglycerols (TAG), similar in size and numbers in vivo and in culture. Temperature-treated (a 15 min exposure once a day to 43ºC for 5 days) and control cells were incubated for 1 h with [3H]20:4 on the 5th day. In the three regions, TAG were faintly labeled, most of the fatty acid being taken up by phospholipids. Caput-derived cells exhibited the highest rate of lipid labeling. Due to heat exposures, caput and corpus cells showed a significant increase in the label from [3H]20:4 in diacylglycerols (DAG), concomitant with a decrease in the label in phosphatidylinositol (PI), phospatidylethanolamine phosphatidylglycerol (PE-PG), and little change in phosphatidylcholine (PC). Quite the opposite occurred in cells from cauda: the label in DAG decreased, while that in PI, PE-PG and PC increased. Our results suggest that epididymal cells are differentially susceptible to short but periodical exposures to mild hyperthermia.Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaL Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularRosarioArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234467Heat stress affects the metabolism of lipids of epididymal epithelial cells; L Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 126-1270327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:11Zoai:ri.conicet.gov.ar:11336/234467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:11.587CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heat stress affects the metabolism of lipids of epididymal epithelial cells
title Heat stress affects the metabolism of lipids of epididymal epithelial cells
spellingShingle Heat stress affects the metabolism of lipids of epididymal epithelial cells
Luquez, Jessica Mariela
HIPERTERMIA
CÉLULAS EPIDIDIMALES
SEGMENTOS EPIDIDIMALES
FOSFOLÍPIDOS
title_short Heat stress affects the metabolism of lipids of epididymal epithelial cells
title_full Heat stress affects the metabolism of lipids of epididymal epithelial cells
title_fullStr Heat stress affects the metabolism of lipids of epididymal epithelial cells
title_full_unstemmed Heat stress affects the metabolism of lipids of epididymal epithelial cells
title_sort Heat stress affects the metabolism of lipids of epididymal epithelial cells
dc.creator.none.fl_str_mv Luquez, Jessica Mariela
Oresti, Gerardo Martin
Valles, Ana Sofia
Aveldaño, Marta Isabel
Furland, Natalia Edith
author Luquez, Jessica Mariela
author_facet Luquez, Jessica Mariela
Oresti, Gerardo Martin
Valles, Ana Sofia
Aveldaño, Marta Isabel
Furland, Natalia Edith
author_role author
author2 Oresti, Gerardo Martin
Valles, Ana Sofia
Aveldaño, Marta Isabel
Furland, Natalia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv HIPERTERMIA
CÉLULAS EPIDIDIMALES
SEGMENTOS EPIDIDIMALES
FOSFOLÍPIDOS
topic HIPERTERMIA
CÉLULAS EPIDIDIMALES
SEGMENTOS EPIDIDIMALES
FOSFOLÍPIDOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Frequent exposures of testes to hyperthermia are known to result in germ cell death, and, although not lethal to Sertoli cells, we previously showed that such exposures damage them structurally and functionally. Here we studied the consequences of heat stress on the epididymis by assessing its effects on epithelial cells from its three regions (caput, corpus, and cauda) in primary culture. All of them had abundant lipid droplets (LD) rich in triacylglycerols (TAG), similar in size and numbers in vivo and in culture. Temperature-treated (a 15 min exposure once a day to 43ºC for 5 days) and control cells were incubated for 1 h with [3H]20:4 on the 5th day. In the three regions, TAG were faintly labeled, most of the fatty acid being taken up by phospholipids. Caput-derived cells exhibited the highest rate of lipid labeling. Due to heat exposures, caput and corpus cells showed a significant increase in the label from [3H]20:4 in diacylglycerols (DAG), concomitant with a decrease in the label in phosphatidylinositol (PI), phospatidylethanolamine phosphatidylglycerol (PE-PG), and little change in phosphatidylcholine (PC). Quite the opposite occurred in cells from cauda: the label in DAG decreased, while that in PI, PE-PG and PC increased. Our results suggest that epididymal cells are differentially susceptible to short but periodical exposures to mild hyperthermia.
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
L Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Frequent exposures of testes to hyperthermia are known to result in germ cell death, and, although not lethal to Sertoli cells, we previously showed that such exposures damage them structurally and functionally. Here we studied the consequences of heat stress on the epididymis by assessing its effects on epithelial cells from its three regions (caput, corpus, and cauda) in primary culture. All of them had abundant lipid droplets (LD) rich in triacylglycerols (TAG), similar in size and numbers in vivo and in culture. Temperature-treated (a 15 min exposure once a day to 43ºC for 5 days) and control cells were incubated for 1 h with [3H]20:4 on the 5th day. In the three regions, TAG were faintly labeled, most of the fatty acid being taken up by phospholipids. Caput-derived cells exhibited the highest rate of lipid labeling. Due to heat exposures, caput and corpus cells showed a significant increase in the label from [3H]20:4 in diacylglycerols (DAG), concomitant with a decrease in the label in phosphatidylinositol (PI), phospatidylethanolamine phosphatidylglycerol (PE-PG), and little change in phosphatidylcholine (PC). Quite the opposite occurred in cells from cauda: the label in DAG decreased, while that in PI, PE-PG and PC increased. Our results suggest that epididymal cells are differentially susceptible to short but periodical exposures to mild hyperthermia.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234467
Heat stress affects the metabolism of lipids of epididymal epithelial cells; L Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 126-127
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234467
identifier_str_mv Heat stress affects the metabolism of lipids of epididymal epithelial cells; L Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Rosario; Argentina; 2014; 126-127
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34067/pdf
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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