The ether-linked lipids of rat epididymis are affected by mild hyperthermia

Autores
Luquez, Jessica Mariela; Santiago Valtierra, Florencia Ximena; Oresti, Gerardo Martin; Aveldaño, Marta Isabel; Furland, Natalia Edith
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
It is widely known that heat stress temporarily suppresses spermatogenesis in the mammalian testis, but the impact on the epididymis is barely known. The aim of this study was to examine the effects of short, repeated once-a-day episodes of hyperthermia (43ºC) on the ether-linked glycerophospholipids (GPL) and triglycerides (TG) of rat epididymis. One-week post-treatment, the expression (mRNA) of alkylglycerone phosphate synthase (AGPS), a key peroxisomal enzyme in the synthesis of these lipids, significantly fell in caput and corpus epididymis. Coincidentally, levels of the plasmalogen precursor, plasmanyletanolamine, decreased. Concurrently, plasmenylethanolamine and plasmenylcholine accumulated in caput, ascribable to injured and motionless cells and sperm collecting in the lumen. Catabolism of such GPL had started in the epididymal epithelium, as suggested by the build-up of ether-linked TG. Between weeks 2 and 6, spermatogenesis restarted in the testis. Although the epididymis was still sperm-free at week 6, its levels of ether-linked GPL and TG were much higher than those of untreated controls, in agreement with the recovery of AGPS expression. Ether-linked TG were formed by de novo synthesis and during GPL breakdown. The presence of spermatozoa in the epididymal lumen apparently plays a regulatory role in the biosynthesis of ether-linked lipids by the epididymal epithelium
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular
Materia
LÍPIDOS CON UNIONES ÉTER
EPIDÍDIMO
HIPERTERMIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246242
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The ether-linked lipids of rat epididymis are affected by mild hyperthermiaLuquez, Jessica MarielaSantiago Valtierra, Florencia XimenaOresti, Gerardo MartinAveldaño, Marta IsabelFurland, Natalia EdithLÍPIDOS CON UNIONES ÉTEREPIDÍDIMOHIPERTERMIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It is widely known that heat stress temporarily suppresses spermatogenesis in the mammalian testis, but the impact on the epididymis is barely known. The aim of this study was to examine the effects of short, repeated once-a-day episodes of hyperthermia (43ºC) on the ether-linked glycerophospholipids (GPL) and triglycerides (TG) of rat epididymis. One-week post-treatment, the expression (mRNA) of alkylglycerone phosphate synthase (AGPS), a key peroxisomal enzyme in the synthesis of these lipids, significantly fell in caput and corpus epididymis. Coincidentally, levels of the plasmalogen precursor, plasmanyletanolamine, decreased. Concurrently, plasmenylethanolamine and plasmenylcholine accumulated in caput, ascribable to injured and motionless cells and sperm collecting in the lumen. Catabolism of such GPL had started in the epididymal epithelium, as suggested by the build-up of ether-linked TG. Between weeks 2 and 6, spermatogenesis restarted in the testis. Although the epididymis was still sperm-free at week 6, its levels of ether-linked GPL and TG were much higher than those of untreated controls, in agreement with the recovery of AGPS expression. Ether-linked TG were formed by de novo synthesis and during GPL breakdown. The presence of spermatozoa in the epididymal lumen apparently plays a regulatory role in the biosynthesis of ether-linked lipids by the epididymal epitheliumFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularCórdobaArgentinaSociedad Argentina de Investigacion en Bioquimica y Biologia MolecularTech Science Press2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246242The ether-linked lipids of rat epididymis are affected by mild hyperthermia; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 36-360327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:03Zoai:ri.conicet.gov.ar:11336/246242instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:03.538CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The ether-linked lipids of rat epididymis are affected by mild hyperthermia
title The ether-linked lipids of rat epididymis are affected by mild hyperthermia
spellingShingle The ether-linked lipids of rat epididymis are affected by mild hyperthermia
Luquez, Jessica Mariela
LÍPIDOS CON UNIONES ÉTER
EPIDÍDIMO
HIPERTERMIA
title_short The ether-linked lipids of rat epididymis are affected by mild hyperthermia
title_full The ether-linked lipids of rat epididymis are affected by mild hyperthermia
title_fullStr The ether-linked lipids of rat epididymis are affected by mild hyperthermia
title_full_unstemmed The ether-linked lipids of rat epididymis are affected by mild hyperthermia
title_sort The ether-linked lipids of rat epididymis are affected by mild hyperthermia
dc.creator.none.fl_str_mv Luquez, Jessica Mariela
Santiago Valtierra, Florencia Ximena
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author Luquez, Jessica Mariela
author_facet Luquez, Jessica Mariela
Santiago Valtierra, Florencia Ximena
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author_role author
author2 Santiago Valtierra, Florencia Ximena
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv LÍPIDOS CON UNIONES ÉTER
EPIDÍDIMO
HIPERTERMIA
topic LÍPIDOS CON UNIONES ÉTER
EPIDÍDIMO
HIPERTERMIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is widely known that heat stress temporarily suppresses spermatogenesis in the mammalian testis, but the impact on the epididymis is barely known. The aim of this study was to examine the effects of short, repeated once-a-day episodes of hyperthermia (43ºC) on the ether-linked glycerophospholipids (GPL) and triglycerides (TG) of rat epididymis. One-week post-treatment, the expression (mRNA) of alkylglycerone phosphate synthase (AGPS), a key peroxisomal enzyme in the synthesis of these lipids, significantly fell in caput and corpus epididymis. Coincidentally, levels of the plasmalogen precursor, plasmanyletanolamine, decreased. Concurrently, plasmenylethanolamine and plasmenylcholine accumulated in caput, ascribable to injured and motionless cells and sperm collecting in the lumen. Catabolism of such GPL had started in the epididymal epithelium, as suggested by the build-up of ether-linked TG. Between weeks 2 and 6, spermatogenesis restarted in the testis. Although the epididymis was still sperm-free at week 6, its levels of ether-linked GPL and TG were much higher than those of untreated controls, in agreement with the recovery of AGPS expression. Ether-linked TG were formed by de novo synthesis and during GPL breakdown. The presence of spermatozoa in the epididymal lumen apparently plays a regulatory role in the biosynthesis of ether-linked lipids by the epididymal epithelium
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular
description It is widely known that heat stress temporarily suppresses spermatogenesis in the mammalian testis, but the impact on the epididymis is barely known. The aim of this study was to examine the effects of short, repeated once-a-day episodes of hyperthermia (43ºC) on the ether-linked glycerophospholipids (GPL) and triglycerides (TG) of rat epididymis. One-week post-treatment, the expression (mRNA) of alkylglycerone phosphate synthase (AGPS), a key peroxisomal enzyme in the synthesis of these lipids, significantly fell in caput and corpus epididymis. Coincidentally, levels of the plasmalogen precursor, plasmanyletanolamine, decreased. Concurrently, plasmenylethanolamine and plasmenylcholine accumulated in caput, ascribable to injured and motionless cells and sperm collecting in the lumen. Catabolism of such GPL had started in the epididymal epithelium, as suggested by the build-up of ether-linked TG. Between weeks 2 and 6, spermatogenesis restarted in the testis. Although the epididymis was still sperm-free at week 6, its levels of ether-linked GPL and TG were much higher than those of untreated controls, in agreement with the recovery of AGPS expression. Ether-linked TG were formed by de novo synthesis and during GPL breakdown. The presence of spermatozoa in the epididymal lumen apparently plays a regulatory role in the biosynthesis of ether-linked lipids by the epididymal epithelium
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246242
The ether-linked lipids of rat epididymis are affected by mild hyperthermia; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 36-36
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246242
identifier_str_mv The ether-linked lipids of rat epididymis are affected by mild hyperthermia; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 36-36
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
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