CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation

Autores
Carvajal, Guillermo; Brukman, Nicolás Gastón; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Guazzone, Vanesa Anabella; Lustig, Livia; Breton, Sylvie; Cuasnicú, Patricia S.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Epididymal CRISP1 and CRISP4 associate with the sperm surface during maturation and are key mediators of fertilization. Whereas single knockout (KO) males for these molecules showed in vitro sperm fertilizing defects but normal fertility, all double KO (DKO) animals for these proteins exhibited impaired in vivo fertilization and fertility. In addition, one third of DKO showed bigger testes and epididymides not observed in single KO. Based on this, in the present work we investigated the mechanisms underlining this DKO phenotype. Histological studies of DKO testes and epididymides showed that whereas mice with normal tissues (Group 1) were not different from controls, those with bigger organs (Group 2) had clear histological defects as well as an abnormal presence of immune cells in the interstitium and lumen. RT-qPCR for different immunomodulator molecules revealed higher levels of Il-6 and Il-10 and a downregulation of Tgf-β in DKO from Group 2 not observed in Group 1. Interestingly, immunofluorescence experiments
using specific markers for each of the different epididymal epithelial cells revealed fewer and shorter basal cell projections in the initial segment known as axiopodia, defects in principal cells and clear cells with an immature phenotype in both groups. Accompanying these epithelial changes, males from both groups also exhibited an increase in intraluminal pH. Altogether, these observations support the relevance of CRISP proteins for male fertility through their involvement in epididymal epithelium differentiation and luminal acidification which are critical for sperm maturation and storage.
Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Battistone, Maria Agustina. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Breton, Sylvie. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina. Harvard Medical School; Estados Unidos
Fil: Cuasnicú, Patricia S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
7th International Conference on the Epididymis
Montreal
Canadá
Epididymis 7 Committee
Materia
CRISP
REPRODUCTION
FERTILITY
EPIDIDIMAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131504
Acceder
id CONICETDig_f9cdaa2261207311976994c85cd39d33
oai_identifier_str oai:ri.conicet.gov.ar:11336/131504
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiationCarvajal, GuillermoBrukman, Nicolás GastónWeigel Muñoz, MarianaBattistone, Maria AgustinaGuazzone, Vanesa AnabellaLustig, LiviaBreton, SylvieCuasnicú, Patricia S.CRISPREPRODUCTIONFERTILITYEPIDIDIMALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Epididymal CRISP1 and CRISP4 associate with the sperm surface during maturation and are key mediators of fertilization. Whereas single knockout (KO) males for these molecules showed in vitro sperm fertilizing defects but normal fertility, all double KO (DKO) animals for these proteins exhibited impaired in vivo fertilization and fertility. In addition, one third of DKO showed bigger testes and epididymides not observed in single KO. Based on this, in the present work we investigated the mechanisms underlining this DKO phenotype. Histological studies of DKO testes and epididymides showed that whereas mice with normal tissues (Group 1) were not different from controls, those with bigger organs (Group 2) had clear histological defects as well as an abnormal presence of immune cells in the interstitium and lumen. RT-qPCR for different immunomodulator molecules revealed higher levels of Il-6 and Il-10 and a downregulation of Tgf-β in DKO from Group 2 not observed in Group 1. Interestingly, immunofluorescence experiments<br />using specific markers for each of the different epididymal epithelial cells revealed fewer and shorter basal cell projections in the initial segment known as axiopodia, defects in principal cells and clear cells with an immature phenotype in both groups. Accompanying these epithelial changes, males from both groups also exhibited an increase in intraluminal pH. Altogether, these observations support the relevance of CRISP proteins for male fertility through their involvement in epididymal epithelium differentiation and luminal acidification which are critical for sperm maturation and storage.Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Battistone, Maria Agustina. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Breton, Sylvie. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina. Harvard Medical School; Estados UnidosFil: Cuasnicú, Patricia S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina7th International Conference on the EpididymisMontrealCanadáEpididymis 7 CommitteeEpididymis 72019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131504CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation; 7th International Conference on the Epididymis; Montreal; Canadá; 2018; 30-30CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicine.mcgill.ca/pharma/robairelab/Documents/Epididymis7_FinalProgram.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:07Zoai:ri.conicet.gov.ar:11336/131504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:07.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
title CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
spellingShingle CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
Carvajal, Guillermo
CRISP
REPRODUCTION
FERTILITY
EPIDIDIMAL
title_short CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
title_full CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
title_fullStr CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
title_full_unstemmed CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
title_sort CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation
dc.creator.none.fl_str_mv Carvajal, Guillermo
Brukman, Nicolás Gastón
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Guazzone, Vanesa Anabella
Lustig, Livia
Breton, Sylvie
Cuasnicú, Patricia S.
author Carvajal, Guillermo
author_facet Carvajal, Guillermo
Brukman, Nicolás Gastón
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Guazzone, Vanesa Anabella
Lustig, Livia
Breton, Sylvie
Cuasnicú, Patricia S.
author_role author
author2 Brukman, Nicolás Gastón
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Guazzone, Vanesa Anabella
Lustig, Livia
Breton, Sylvie
Cuasnicú, Patricia S.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CRISP
REPRODUCTION
FERTILITY
EPIDIDIMAL
topic CRISP
REPRODUCTION
FERTILITY
EPIDIDIMAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Epididymal CRISP1 and CRISP4 associate with the sperm surface during maturation and are key mediators of fertilization. Whereas single knockout (KO) males for these molecules showed in vitro sperm fertilizing defects but normal fertility, all double KO (DKO) animals for these proteins exhibited impaired in vivo fertilization and fertility. In addition, one third of DKO showed bigger testes and epididymides not observed in single KO. Based on this, in the present work we investigated the mechanisms underlining this DKO phenotype. Histological studies of DKO testes and epididymides showed that whereas mice with normal tissues (Group 1) were not different from controls, those with bigger organs (Group 2) had clear histological defects as well as an abnormal presence of immune cells in the interstitium and lumen. RT-qPCR for different immunomodulator molecules revealed higher levels of Il-6 and Il-10 and a downregulation of Tgf-β in DKO from Group 2 not observed in Group 1. Interestingly, immunofluorescence experiments<br />using specific markers for each of the different epididymal epithelial cells revealed fewer and shorter basal cell projections in the initial segment known as axiopodia, defects in principal cells and clear cells with an immature phenotype in both groups. Accompanying these epithelial changes, males from both groups also exhibited an increase in intraluminal pH. Altogether, these observations support the relevance of CRISP proteins for male fertility through their involvement in epididymal epithelium differentiation and luminal acidification which are critical for sperm maturation and storage.
Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Battistone, Maria Agustina. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Breton, Sylvie. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina. Harvard Medical School; Estados Unidos
Fil: Cuasnicú, Patricia S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
7th International Conference on the Epididymis
Montreal
Canadá
Epididymis 7 Committee
description Epididymal CRISP1 and CRISP4 associate with the sperm surface during maturation and are key mediators of fertilization. Whereas single knockout (KO) males for these molecules showed in vitro sperm fertilizing defects but normal fertility, all double KO (DKO) animals for these proteins exhibited impaired in vivo fertilization and fertility. In addition, one third of DKO showed bigger testes and epididymides not observed in single KO. Based on this, in the present work we investigated the mechanisms underlining this DKO phenotype. Histological studies of DKO testes and epididymides showed that whereas mice with normal tissues (Group 1) were not different from controls, those with bigger organs (Group 2) had clear histological defects as well as an abnormal presence of immune cells in the interstitium and lumen. RT-qPCR for different immunomodulator molecules revealed higher levels of Il-6 and Il-10 and a downregulation of Tgf-β in DKO from Group 2 not observed in Group 1. Interestingly, immunofluorescence experiments<br />using specific markers for each of the different epididymal epithelial cells revealed fewer and shorter basal cell projections in the initial segment known as axiopodia, defects in principal cells and clear cells with an immature phenotype in both groups. Accompanying these epithelial changes, males from both groups also exhibited an increase in intraluminal pH. Altogether, these observations support the relevance of CRISP proteins for male fertility through their involvement in epididymal epithelium differentiation and luminal acidification which are critical for sperm maturation and storage.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131504
CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation; 7th International Conference on the Epididymis; Montreal; Canadá; 2018; 30-30
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131504
identifier_str_mv CRISP (Cysteine Rich Secretory Proteins) as novel regulators of epididymal epithelium differentiation; 7th International Conference on the Epididymis; Montreal; Canadá; 2018; 30-30
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicine.mcgill.ca/pharma/robairelab/Documents/Epididymis7_FinalProgram.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Epididymis 7
publisher.none.fl_str_mv Epididymis 7
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782135188324352
score 12.982451

Publicaciones similares