Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells

Autores
Luquez, Jessica Mariela; Oresti, Gerardo Martin; Aveldaño, Marta Isabel; Furland, Natalia Edith
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In rats, epididymal sperm maturation is associated with the acquisition of glycerophospholipids (GPL) with uncommon C20- C24 carbon PUFA of the n-9 series, particularly 22:4n-9-rich plasmenylcholine. Little is known about how the epididymal epithelium participates in this accretion. In this study, three epididymal regions (caput, corpus, cauda) were isolated for lipid and fatty acid analysis. Unexpectedly, the main n-9 PUFA-rich epididymal GPL including plasmalogens were those of ethanolamine rather than those of choline. Epididymal triacylglycerols (TAG) were highly enriched in 18:1n-9, a precursor of 22:4n-9, whereas the ether-linked triglycerides (ADG), as the GPL, were rich in C20-C24 n-9 PUFA (mainly 22:4n-9). The epididymal n-9 PUFA-rich ADG were made up by important proportions of 1-O-alkyl and 1-alk-1´enyl diacylglycerols. This contrasts with the ADG of testis, where the former subclass predominates over the latter and both are rich in 22:5n-6. Although 22:4n-9-rich GPL and ADG were present in the three epididymal segments, they were significantly more concentrated in the corpus, the metabolically most active region of the epididymis. Our results suggest an active involvement of epididymal cells in the lipid remodeling undergone by spermatozoa during their maturation, most of which takes place before the mature gametes are stored in the cauda region.
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argenina de investigación en Bioquímica y Biología Molecular
Materia
EPIDIDYMIS
PLASMALOGENS
ETHER-LINKED TRIGLYCERIDES
n-9 POLYUNSATURATED FATTY ACIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244980
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244980
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cellsLuquez, Jessica MarielaOresti, Gerardo MartinAveldaño, Marta IsabelFurland, Natalia EdithEPIDIDYMISPLASMALOGENSETHER-LINKED TRIGLYCERIDESn-9 POLYUNSATURATED FATTY ACIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In rats, epididymal sperm maturation is associated with the acquisition of glycerophospholipids (GPL) with uncommon C20- C24 carbon PUFA of the n-9 series, particularly 22:4n-9-rich plasmenylcholine. Little is known about how the epididymal epithelium participates in this accretion. In this study, three epididymal regions (caput, corpus, cauda) were isolated for lipid and fatty acid analysis. Unexpectedly, the main n-9 PUFA-rich epididymal GPL including plasmalogens were those of ethanolamine rather than those of choline. Epididymal triacylglycerols (TAG) were highly enriched in 18:1n-9, a precursor of 22:4n-9, whereas the ether-linked triglycerides (ADG), as the GPL, were rich in C20-C24 n-9 PUFA (mainly 22:4n-9). The epididymal n-9 PUFA-rich ADG were made up by important proportions of 1-O-alkyl and 1-alk-1´enyl diacylglycerols. This contrasts with the ADG of testis, where the former subclass predominates over the latter and both are rich in 22:5n-6. Although 22:4n-9-rich GPL and ADG were present in the three epididymal segments, they were significantly more concentrated in the corpus, the metabolically most active region of the epididymis. Our results suggest an active involvement of epididymal cells in the lipid remodeling undergone by spermatozoa during their maturation, most of which takes place before the mature gametes are stored in the cauda region.Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argenina de investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244980Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells; XXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 91-910327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:51Zoai:ri.conicet.gov.ar:11336/244980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:51.901CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
title Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
spellingShingle Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
Luquez, Jessica Mariela
EPIDIDYMIS
PLASMALOGENS
ETHER-LINKED TRIGLYCERIDES
n-9 POLYUNSATURATED FATTY ACIDS
title_short Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
title_full Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
title_fullStr Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
title_full_unstemmed Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
title_sort Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells
dc.creator.none.fl_str_mv Luquez, Jessica Mariela
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author Luquez, Jessica Mariela
author_facet Luquez, Jessica Mariela
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author_role author
author2 Oresti, Gerardo Martin
Aveldaño, Marta Isabel
Furland, Natalia Edith
author2_role author
author
author
dc.subject.none.fl_str_mv EPIDIDYMIS
PLASMALOGENS
ETHER-LINKED TRIGLYCERIDES
n-9 POLYUNSATURATED FATTY ACIDS
topic EPIDIDYMIS
PLASMALOGENS
ETHER-LINKED TRIGLYCERIDES
n-9 POLYUNSATURATED FATTY ACIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In rats, epididymal sperm maturation is associated with the acquisition of glycerophospholipids (GPL) with uncommon C20- C24 carbon PUFA of the n-9 series, particularly 22:4n-9-rich plasmenylcholine. Little is known about how the epididymal epithelium participates in this accretion. In this study, three epididymal regions (caput, corpus, cauda) were isolated for lipid and fatty acid analysis. Unexpectedly, the main n-9 PUFA-rich epididymal GPL including plasmalogens were those of ethanolamine rather than those of choline. Epididymal triacylglycerols (TAG) were highly enriched in 18:1n-9, a precursor of 22:4n-9, whereas the ether-linked triglycerides (ADG), as the GPL, were rich in C20-C24 n-9 PUFA (mainly 22:4n-9). The epididymal n-9 PUFA-rich ADG were made up by important proportions of 1-O-alkyl and 1-alk-1´enyl diacylglycerols. This contrasts with the ADG of testis, where the former subclass predominates over the latter and both are rich in 22:5n-6. Although 22:4n-9-rich GPL and ADG were present in the three epididymal segments, they were significantly more concentrated in the corpus, the metabolically most active region of the epididymis. Our results suggest an active involvement of epididymal cells in the lipid remodeling undergone by spermatozoa during their maturation, most of which takes place before the mature gametes are stored in the cauda region.
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argenina de investigación en Bioquímica y Biología Molecular
description In rats, epididymal sperm maturation is associated with the acquisition of glycerophospholipids (GPL) with uncommon C20- C24 carbon PUFA of the n-9 series, particularly 22:4n-9-rich plasmenylcholine. Little is known about how the epididymal epithelium participates in this accretion. In this study, three epididymal regions (caput, corpus, cauda) were isolated for lipid and fatty acid analysis. Unexpectedly, the main n-9 PUFA-rich epididymal GPL including plasmalogens were those of ethanolamine rather than those of choline. Epididymal triacylglycerols (TAG) were highly enriched in 18:1n-9, a precursor of 22:4n-9, whereas the ether-linked triglycerides (ADG), as the GPL, were rich in C20-C24 n-9 PUFA (mainly 22:4n-9). The epididymal n-9 PUFA-rich ADG were made up by important proportions of 1-O-alkyl and 1-alk-1´enyl diacylglycerols. This contrasts with the ADG of testis, where the former subclass predominates over the latter and both are rich in 22:5n-6. Although 22:4n-9-rich GPL and ADG were present in the three epididymal segments, they were significantly more concentrated in the corpus, the metabolically most active region of the epididymis. Our results suggest an active involvement of epididymal cells in the lipid remodeling undergone by spermatozoa during their maturation, most of which takes place before the mature gametes are stored in the cauda region.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244980
Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells; XXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 91-91
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244980
identifier_str_mv Lipids with long chain polyenoic fatty acids (PUFA) of the n-9 series in rat epididymal cells; XXVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 91-91
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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