The Tomato Terpene Synthase Gene Family
- Autores
- Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; Bleeker, Petra M.; Abatedaga, Maria Ines de Los Angeles; Matsuba, Yuki; Bonini, Megan E.; Schilmiller, Anthony L.; Last, Robert L.; Schuurink, Robert C.; Pichersky, Eran
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Falara, Vasiliki. University of Michigan; Estados Unidos
Fil: Akhtar, Tariq A.. University of Michigan; Estados Unidos
Fil: Nguyen, Thuong, T. H.. University of Michigan; Estados Unidos
Fil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados Unidos
Fil: Matsuba, Yuki. University of Michigan; Estados Unidos
Fil: Bonini, Megan E.. University of Michigan; Estados Unidos
Fil: Schilmiller, Anthony L.. Michigan State University; Estados Unidos
Fil: Last, Robert L.. Michigan State University; Estados Unidos
Fil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Pichersky, Eran. University of Michigan; Estados Unidos - Materia
-
Biochemistry
Plants
Metabolism
Terpenes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/52531
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The Tomato Terpene Synthase Gene FamilyAbatedaga, Maria Ines de Los AngelesFalara, VasilikiAkhtar, Tariq A.Nguyen, Thuong, T. H.Spyropoulou, Eleni A.Bleeker, Petra M.Abatedaga, Maria Ines de Los AngelesMatsuba, YukiBonini, Megan E.Schilmiller, Anthony L.Last, Robert L.Schuurink, Robert C.Pichersky, EranBiochemistryPlantsMetabolismTerpeneshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Falara, Vasiliki. University of Michigan; Estados UnidosFil: Akhtar, Tariq A.. University of Michigan; Estados UnidosFil: Nguyen, Thuong, T. H.. University of Michigan; Estados UnidosFil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países BajosFil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países BajosFil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados UnidosFil: Matsuba, Yuki. University of Michigan; Estados UnidosFil: Bonini, Megan E.. University of Michigan; Estados UnidosFil: Schilmiller, Anthony L.. Michigan State University; Estados UnidosFil: Last, Robert L.. Michigan State University; Estados UnidosFil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países BajosFil: Pichersky, Eran. University of Michigan; Estados UnidosAmerican Society of Plant Biologist2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52531Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-7890032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/www.plantphysiol.org/cgi/doi/10.1104/pp.111.179648info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.111.179648info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:53Zoai:ri.conicet.gov.ar:11336/52531instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:53.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The Tomato Terpene Synthase Gene Family |
title |
The Tomato Terpene Synthase Gene Family |
spellingShingle |
The Tomato Terpene Synthase Gene Family Abatedaga, Maria Ines de Los Angeles Biochemistry Plants Metabolism Terpenes |
title_short |
The Tomato Terpene Synthase Gene Family |
title_full |
The Tomato Terpene Synthase Gene Family |
title_fullStr |
The Tomato Terpene Synthase Gene Family |
title_full_unstemmed |
The Tomato Terpene Synthase Gene Family |
title_sort |
The Tomato Terpene Synthase Gene Family |
dc.creator.none.fl_str_mv |
Abatedaga, Maria Ines de Los Angeles Falara, Vasiliki Akhtar, Tariq A. Nguyen, Thuong, T. H. Spyropoulou, Eleni A. Bleeker, Petra M. Abatedaga, Maria Ines de Los Angeles Matsuba, Yuki Bonini, Megan E. Schilmiller, Anthony L. Last, Robert L. Schuurink, Robert C. Pichersky, Eran |
author |
Abatedaga, Maria Ines de Los Angeles |
author_facet |
Abatedaga, Maria Ines de Los Angeles Falara, Vasiliki Akhtar, Tariq A. Nguyen, Thuong, T. H. Spyropoulou, Eleni A. Bleeker, Petra M. Matsuba, Yuki Bonini, Megan E. Schilmiller, Anthony L. Last, Robert L. Schuurink, Robert C. Pichersky, Eran |
author_role |
author |
author2 |
Falara, Vasiliki Akhtar, Tariq A. Nguyen, Thuong, T. H. Spyropoulou, Eleni A. Bleeker, Petra M. Matsuba, Yuki Bonini, Megan E. Schilmiller, Anthony L. Last, Robert L. Schuurink, Robert C. Pichersky, Eran |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Biochemistry Plants Metabolism Terpenes |
topic |
Biochemistry Plants Metabolism Terpenes |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far. Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Falara, Vasiliki. University of Michigan; Estados Unidos Fil: Akhtar, Tariq A.. University of Michigan; Estados Unidos Fil: Nguyen, Thuong, T. H.. University of Michigan; Estados Unidos Fil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países Bajos Fil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países Bajos Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados Unidos Fil: Matsuba, Yuki. University of Michigan; Estados Unidos Fil: Bonini, Megan E.. University of Michigan; Estados Unidos Fil: Schilmiller, Anthony L.. Michigan State University; Estados Unidos Fil: Last, Robert L.. Michigan State University; Estados Unidos Fil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países Bajos Fil: Pichersky, Eran. University of Michigan; Estados Unidos |
description |
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/52531 Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-789 0032-0889 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/52531 |
identifier_str_mv |
Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-789 0032-0889 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/www.plantphysiol.org/cgi/doi/10.1104/pp.111.179648 info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.111.179648 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Plant Biologist |
publisher.none.fl_str_mv |
American Society of Plant Biologist |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |