The Tomato Terpene Synthase Gene Family

Autores
Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; Bleeker, Petra M.; Abatedaga, Maria Ines de Los Angeles; Matsuba, Yuki; Bonini, Megan E.; Schilmiller, Anthony L.; Last, Robert L.; Schuurink, Robert C.; Pichersky, Eran
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Falara, Vasiliki. University of Michigan; Estados Unidos
Fil: Akhtar, Tariq A.. University of Michigan; Estados Unidos
Fil: Nguyen, Thuong, T. H.. University of Michigan; Estados Unidos
Fil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados Unidos
Fil: Matsuba, Yuki. University of Michigan; Estados Unidos
Fil: Bonini, Megan E.. University of Michigan; Estados Unidos
Fil: Schilmiller, Anthony L.. Michigan State University; Estados Unidos
Fil: Last, Robert L.. Michigan State University; Estados Unidos
Fil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Pichersky, Eran. University of Michigan; Estados Unidos
Materia
Biochemistry
Plants
Metabolism
Terpenes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52531
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52531
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Tomato Terpene Synthase Gene FamilyAbatedaga, Maria Ines de Los AngelesFalara, VasilikiAkhtar, Tariq A.Nguyen, Thuong, T. H.Spyropoulou, Eleni A.Bleeker, Petra M.Abatedaga, Maria Ines de Los AngelesMatsuba, YukiBonini, Megan E.Schilmiller, Anthony L.Last, Robert L.Schuurink, Robert C.Pichersky, EranBiochemistryPlantsMetabolismTerpeneshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Falara, Vasiliki. University of Michigan; Estados UnidosFil: Akhtar, Tariq A.. University of Michigan; Estados UnidosFil: Nguyen, Thuong, T. H.. University of Michigan; Estados UnidosFil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países BajosFil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países BajosFil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados UnidosFil: Matsuba, Yuki. University of Michigan; Estados UnidosFil: Bonini, Megan E.. University of Michigan; Estados UnidosFil: Schilmiller, Anthony L.. Michigan State University; Estados UnidosFil: Last, Robert L.. Michigan State University; Estados UnidosFil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países BajosFil: Pichersky, Eran. University of Michigan; Estados UnidosAmerican Society of Plant Biologist2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52531Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-7890032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/www.plantphysiol.org/cgi/doi/10.1104/pp.111.179648info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.111.179648info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:45Zoai:ri.conicet.gov.ar:11336/52531instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:46.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Tomato Terpene Synthase Gene Family
title The Tomato Terpene Synthase Gene Family
spellingShingle The Tomato Terpene Synthase Gene Family
Abatedaga, Maria Ines de Los Angeles
Biochemistry
Plants
Metabolism
Terpenes
title_short The Tomato Terpene Synthase Gene Family
title_full The Tomato Terpene Synthase Gene Family
title_fullStr The Tomato Terpene Synthase Gene Family
title_full_unstemmed The Tomato Terpene Synthase Gene Family
title_sort The Tomato Terpene Synthase Gene Family
dc.creator.none.fl_str_mv Abatedaga, Maria Ines de Los Angeles
Falara, Vasiliki
Akhtar, Tariq A.
Nguyen, Thuong, T. H.
Spyropoulou, Eleni A.
Bleeker, Petra M.
Abatedaga, Maria Ines de Los Angeles
Matsuba, Yuki
Bonini, Megan E.
Schilmiller, Anthony L.
Last, Robert L.
Schuurink, Robert C.
Pichersky, Eran
author Abatedaga, Maria Ines de Los Angeles
author_facet Abatedaga, Maria Ines de Los Angeles
Falara, Vasiliki
Akhtar, Tariq A.
Nguyen, Thuong, T. H.
Spyropoulou, Eleni A.
Bleeker, Petra M.
Matsuba, Yuki
Bonini, Megan E.
Schilmiller, Anthony L.
Last, Robert L.
Schuurink, Robert C.
Pichersky, Eran
author_role author
author2 Falara, Vasiliki
Akhtar, Tariq A.
Nguyen, Thuong, T. H.
Spyropoulou, Eleni A.
Bleeker, Petra M.
Matsuba, Yuki
Bonini, Megan E.
Schilmiller, Anthony L.
Last, Robert L.
Schuurink, Robert C.
Pichersky, Eran
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biochemistry
Plants
Metabolism
Terpenes
topic Biochemistry
Plants
Metabolism
Terpenes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Falara, Vasiliki. University of Michigan; Estados Unidos
Fil: Akhtar, Tariq A.. University of Michigan; Estados Unidos
Fil: Nguyen, Thuong, T. H.. University of Michigan; Estados Unidos
Fil: Spyropoulou, Eleni A.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Bleeker, Petra M.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Abatedaga, Maria Ines de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. University of Michigan; Estados Unidos
Fil: Matsuba, Yuki. University of Michigan; Estados Unidos
Fil: Bonini, Megan E.. University of Michigan; Estados Unidos
Fil: Schilmiller, Anthony L.. Michigan State University; Estados Unidos
Fil: Last, Robert L.. Michigan State University; Estados Unidos
Fil: Schuurink, Robert C.. Swammerdam Institute of Life Sciences; Países Bajos
Fil: Pichersky, Eran. University of Michigan; Estados Unidos
description Compounds of the terpenoid class play numerous roles in the interactions of plants with their environment, such as attracting pollinators and defending the plant against pests. We show here that the genome of cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are functional or potentially functional. Of these 29 TPS genes, 26 were expressed in at least some organs or tissues of the plant. The enzymatic functions of eight of the TPS proteins were previously reported, and here we report the specific in vitro catalytic activity of 10 additional tomato terpene synthases. Many of the tomato TPS genes are found in clusters, notably on chromosomes 1, 2, 6, 8, and 10. All TPS family clades previously identified in angiosperms are also present in tomato. The largest clade of functional TPS genes found in tomato, with 12 members, is the TPS-a clade, and it appears to encode only sesquiterpene synthases, one of which is localized to the mitochondria, while the rest are likely cytosolic. A few additional sesquiterpene synthases are encoded by TPS-b clade genes. Some of the tomato sesquiterpene synthases use z,z-farnesyl diphosphate in vitro as well, or more efficiently than, the e,e- farnesyl diphosphate substrate. Genes encoding monoterpene synthases are also prevalent, and they fall into three clades: TPS-b, TPS-g, and TPS-e/f. With the exception of two enzymes involved in the synthesis of ent-kaurene, the precursor of gibberellins, no other tomato TPS genes could be demonstrated to encode diterpene synthases so far.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52531
Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-789
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52531
identifier_str_mv Abatedaga, Maria Ines de Los Angeles; Falara, Vasiliki; Akhtar, Tariq A.; Nguyen, Thuong, T. H.; Spyropoulou, Eleni A.; et al.; The Tomato Terpene Synthase Gene Family; American Society of Plant Biologist; Plant Physiology; 157; 2; 10-2011; 770-789
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/www.plantphysiol.org/cgi/doi/10.1104/pp.111.179648
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.111.179648
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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