The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato

Autores
Kang, Jin-Ho; McRoberts, John; Shi, Feng; Moreno, Javier Edgardo; Jones, A. Daniel; Howe, Gregg A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flavonoids and terpenoids are derived from distinct metabolic pathways but nevertheless serve complementary roles in mediating plant interactions with the environment. Here, we show that glandular trichomes of the anthocyanin free(af) mutant of cultivated tomato (Solanum lycopersicum) fail to accumulate both flavonoids and terpenoids. This pleiotropic metabolic deficiency was associated with loss of resistance to native populations of coleopteran herbivores under field conditions. We demonstrate that Af encodes an isoform (SlCHI1) of the flavonoid biosynthetic enzyme chalcone isomerase (CHI), which catalyzes the conversion of naringenin chalcone to naringenin and is strictly required for flavonoid production in multiple tissues of tomato. Expression of the wild-type SlCHI1gene from its native promoter complemented the anthocyanin deficiency in af. Unexpectedly, the SlCHI1transgene also complemented the defect in terpenoid production in glandular trichomes. Our results establish a key role for SlCHI1 in flavonoid production in tomato and reveal a link between CHI1 and terpenoid production. Metabolic coordination of the flavonoid and terpenoid pathways may serve to optimize the function of trichome glands in dynamic environments. 
Fil: Kang, Jin-Ho. Michigan State University; Estados Unidos
Fil: McRoberts, John. Michigan State University; Estados Unidos
Fil: Shi, Feng. Michigan State University; Estados Unidos
Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. University Of Michigan; Estados Unidos
Fil: Jones, A. Daniel. Michigan State University; Estados Unidos
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
Materia
Chalcone Isomerase
Flavonoid
Terpenoid
Coordination
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13321
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13321
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomatoKang, Jin-HoMcRoberts, JohnShi, FengMoreno, Javier EdgardoJones, A. DanielHowe, Gregg A.Chalcone IsomeraseFlavonoidTerpenoidCoordinationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flavonoids and terpenoids are derived from distinct metabolic pathways but nevertheless serve complementary roles in mediating plant interactions with the environment. Here, we show that glandular trichomes of the anthocyanin free(af) mutant of cultivated tomato (Solanum lycopersicum) fail to accumulate both flavonoids and terpenoids. This pleiotropic metabolic deficiency was associated with loss of resistance to native populations of coleopteran herbivores under field conditions. We demonstrate that Af encodes an isoform (SlCHI1) of the flavonoid biosynthetic enzyme chalcone isomerase (CHI), which catalyzes the conversion of naringenin chalcone to naringenin and is strictly required for flavonoid production in multiple tissues of tomato. Expression of the wild-type SlCHI1gene from its native promoter complemented the anthocyanin deficiency in af. Unexpectedly, the SlCHI1transgene also complemented the defect in terpenoid production in glandular trichomes. Our results establish a key role for SlCHI1 in flavonoid production in tomato and reveal a link between CHI1 and terpenoid production. Metabolic coordination of the flavonoid and terpenoid pathways may serve to optimize the function of trichome glands in dynamic environments. Fil: Kang, Jin-Ho. Michigan State University; Estados UnidosFil: McRoberts, John. Michigan State University; Estados UnidosFil: Shi, Feng. Michigan State University; Estados UnidosFil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. University Of Michigan; Estados UnidosFil: Jones, A. Daniel. Michigan State University; Estados UnidosFil: Howe, Gregg A.. Michigan State University; Estados UnidosAmerican Society Of Plant Biologist2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13321Kang, Jin-Ho; McRoberts, John; Shi, Feng; Moreno, Javier Edgardo; Jones, A. Daniel; et al.; The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato; American Society Of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1161-11740032-0889enginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/3/1161info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1104/pp.113.233395info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:41Zoai:ri.conicet.gov.ar:11336/13321instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:41.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
title The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
spellingShingle The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
Kang, Jin-Ho
Chalcone Isomerase
Flavonoid
Terpenoid
Coordination
title_short The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
title_full The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
title_fullStr The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
title_full_unstemmed The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
title_sort The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato
dc.creator.none.fl_str_mv Kang, Jin-Ho
McRoberts, John
Shi, Feng
Moreno, Javier Edgardo
Jones, A. Daniel
Howe, Gregg A.
author Kang, Jin-Ho
author_facet Kang, Jin-Ho
McRoberts, John
Shi, Feng
Moreno, Javier Edgardo
Jones, A. Daniel
Howe, Gregg A.
author_role author
author2 McRoberts, John
Shi, Feng
Moreno, Javier Edgardo
Jones, A. Daniel
Howe, Gregg A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chalcone Isomerase
Flavonoid
Terpenoid
Coordination
topic Chalcone Isomerase
Flavonoid
Terpenoid
Coordination
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flavonoids and terpenoids are derived from distinct metabolic pathways but nevertheless serve complementary roles in mediating plant interactions with the environment. Here, we show that glandular trichomes of the anthocyanin free(af) mutant of cultivated tomato (Solanum lycopersicum) fail to accumulate both flavonoids and terpenoids. This pleiotropic metabolic deficiency was associated with loss of resistance to native populations of coleopteran herbivores under field conditions. We demonstrate that Af encodes an isoform (SlCHI1) of the flavonoid biosynthetic enzyme chalcone isomerase (CHI), which catalyzes the conversion of naringenin chalcone to naringenin and is strictly required for flavonoid production in multiple tissues of tomato. Expression of the wild-type SlCHI1gene from its native promoter complemented the anthocyanin deficiency in af. Unexpectedly, the SlCHI1transgene also complemented the defect in terpenoid production in glandular trichomes. Our results establish a key role for SlCHI1 in flavonoid production in tomato and reveal a link between CHI1 and terpenoid production. Metabolic coordination of the flavonoid and terpenoid pathways may serve to optimize the function of trichome glands in dynamic environments. 
Fil: Kang, Jin-Ho. Michigan State University; Estados Unidos
Fil: McRoberts, John. Michigan State University; Estados Unidos
Fil: Shi, Feng. Michigan State University; Estados Unidos
Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. University Of Michigan; Estados Unidos
Fil: Jones, A. Daniel. Michigan State University; Estados Unidos
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
description Flavonoids and terpenoids are derived from distinct metabolic pathways but nevertheless serve complementary roles in mediating plant interactions with the environment. Here, we show that glandular trichomes of the anthocyanin free(af) mutant of cultivated tomato (Solanum lycopersicum) fail to accumulate both flavonoids and terpenoids. This pleiotropic metabolic deficiency was associated with loss of resistance to native populations of coleopteran herbivores under field conditions. We demonstrate that Af encodes an isoform (SlCHI1) of the flavonoid biosynthetic enzyme chalcone isomerase (CHI), which catalyzes the conversion of naringenin chalcone to naringenin and is strictly required for flavonoid production in multiple tissues of tomato. Expression of the wild-type SlCHI1gene from its native promoter complemented the anthocyanin deficiency in af. Unexpectedly, the SlCHI1transgene also complemented the defect in terpenoid production in glandular trichomes. Our results establish a key role for SlCHI1 in flavonoid production in tomato and reveal a link between CHI1 and terpenoid production. Metabolic coordination of the flavonoid and terpenoid pathways may serve to optimize the function of trichome glands in dynamic environments. 
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13321
Kang, Jin-Ho; McRoberts, John; Shi, Feng; Moreno, Javier Edgardo; Jones, A. Daniel; et al.; The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato; American Society Of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1161-1174
0032-0889
url http://hdl.handle.net/11336/13321
identifier_str_mv Kang, Jin-Ho; McRoberts, John; Shi, Feng; Moreno, Javier Edgardo; Jones, A. Daniel; et al.; The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato; American Society Of Plant Biologist; Plant Physiology; 164; 3; 1-2014; 1161-1174
0032-0889
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/164/3/1161
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1104/pp.113.233395
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society Of Plant Biologist
publisher.none.fl_str_mv American Society Of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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