Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
- Autores
- Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.
Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina - Materia
-
GRAPE LEAF TISSUE
VITIS VINIFERA
VITACEAE
UV-B RADIATION
TERPENE METABOLISM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/104668
Ver los metadatos del registro completo
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Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiationGil, MarianaPontin, Mariela AnaBerli, Federico JavierBottini, Ambrosio RubénPiccoli, Patricia NoemíGRAPE LEAF TISSUEVITIS VINIFERAVITACEAEUV-B RADIATIONTERPENE METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaPergamon-Elsevier Science Ltd2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104668Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-980031-94221873-3700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2011.12.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0031942211005747info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:35Zoai:ri.conicet.gov.ar:11336/104668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:35.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| title |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| spellingShingle |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation Gil, Mariana GRAPE LEAF TISSUE VITIS VINIFERA VITACEAE UV-B RADIATION TERPENE METABOLISM |
| title_short |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| title_full |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| title_fullStr |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| title_full_unstemmed |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| title_sort |
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation |
| dc.creator.none.fl_str_mv |
Gil, Mariana Pontin, Mariela Ana Berli, Federico Javier Bottini, Ambrosio Rubén Piccoli, Patricia Noemí |
| author |
Gil, Mariana |
| author_facet |
Gil, Mariana Pontin, Mariela Ana Berli, Federico Javier Bottini, Ambrosio Rubén Piccoli, Patricia Noemí |
| author_role |
author |
| author2 |
Pontin, Mariela Ana Berli, Federico Javier Bottini, Ambrosio Rubén Piccoli, Patricia Noemí |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
GRAPE LEAF TISSUE VITIS VINIFERA VITACEAE UV-B RADIATION TERPENE METABOLISM |
| topic |
GRAPE LEAF TISSUE VITIS VINIFERA VITACEAE UV-B RADIATION TERPENE METABOLISM |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity. Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina |
| description |
This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/104668 Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-98 0031-9422 1873-3700 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/104668 |
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Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-98 0031-9422 1873-3700 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2011.12.011 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0031942211005747 |
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openAccess |
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Pergamon-Elsevier Science Ltd |
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Pergamon-Elsevier Science Ltd |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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