Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation

Autores
Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.
Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
GRAPE LEAF TISSUE
VITIS VINIFERA
VITACEAE
UV-B RADIATION
TERPENE METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104668
Acceder
id CONICETDig_0cbc02c94a3e70b3dd5dee4c9140dfcc
oai_identifier_str oai:ri.conicet.gov.ar:11336/104668
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiationGil, MarianaPontin, Mariela AnaBerli, Federico JavierBottini, Ambrosio RubénPiccoli, Patricia NoemíGRAPE LEAF TISSUEVITIS VINIFERAVITACEAEUV-B RADIATIONTERPENE METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaPergamon-Elsevier Science Ltd2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104668Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-980031-94221873-3700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2011.12.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0031942211005747info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:07Zoai:ri.conicet.gov.ar:11336/104668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:07.629CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
title Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
spellingShingle Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
Gil, Mariana
GRAPE LEAF TISSUE
VITIS VINIFERA
VITACEAE
UV-B RADIATION
TERPENE METABOLISM
title_short Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
title_full Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
title_fullStr Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
title_full_unstemmed Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
title_sort Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
dc.creator.none.fl_str_mv Gil, Mariana
Pontin, Mariela Ana
Berli, Federico Javier
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author Gil, Mariana
author_facet Gil, Mariana
Pontin, Mariela Ana
Berli, Federico Javier
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author_role author
author2 Pontin, Mariela Ana
Berli, Federico Javier
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author2_role author
author
author
author
dc.subject.none.fl_str_mv GRAPE LEAF TISSUE
VITIS VINIFERA
VITACEAE
UV-B RADIATION
TERPENE METABOLISM
topic GRAPE LEAF TISSUE
VITIS VINIFERA
VITACEAE
UV-B RADIATION
TERPENE METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.
Fil: Gil, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description This study investigated the terpene profiles as determined by GC-EIMS analysis of in vitro cultured plants of Vitis vinifera exposed to a "field-like" dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B treatment increased levels of the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more notable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. By contrast, accumulation of compounds with antioxidant properties, diterpenes alpha and gamma tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, alpha-pinene and terpinolene had maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanism of defense. The adaptative responses induced by relatively low UV-B irradiations as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104668
Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-98
0031-9422
1873-3700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104668
identifier_str_mv Gil, Mariana; Pontin, Mariela Ana; Berli, Federico Javier; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí; Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation; Pergamon-Elsevier Science Ltd; Phytochemistry; 77; 1-2012; 89-98
0031-9422
1873-3700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2011.12.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0031942211005747
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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