Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum

Autores
Pontin, Mariela Ana; Piccoli, Patricia Noemí; Burba, Jose Luis; Bottini, Ambrosio Rubén
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity
EEA La Consulta
Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Phytochemistry 115 :152-160. (July 2015)
Materia
Ecología de la planta
Allium Sativum
Ajo
Terpenoidos
Sclerotium Cepivorum
Propiedades Antimicósicas
Garlic
Terpenoids
Antifungal Properties
Terpenos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1161
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1161
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorumPontin, Mariela AnaPiccoli, Patricia NoemíBurba, Jose LuisBottini, Ambrosio RubénEcología de la plantaAllium SativumAjoTerpenoidosSclerotium CepivorumPropiedades AntimicósicasGarlicTerpenoidsAntifungal PropertiesTerpenosThis study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrityEEA La ConsultaFil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina2017-09-07T12:46:16Z2017-09-07T12:46:16Z2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1161http://ri.conicet.gov.ar/handle/11336/22405http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub0031-9422 (Print)1873-3700 (Online)https://doi.org/10.1016/j.phytochem.2015.02.003Phytochemistry 115 :152-160. (July 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengMendoza (province)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:09Zoai:localhost:20.500.12123/1161instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.285INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
spellingShingle Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
Pontin, Mariela Ana
Ecología de la planta
Allium Sativum
Ajo
Terpenoidos
Sclerotium Cepivorum
Propiedades Antimicósicas
Garlic
Terpenoids
Antifungal Properties
Terpenos
title_short Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_full Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_fullStr Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_full_unstemmed Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_sort Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
dc.creator.none.fl_str_mv Pontin, Mariela Ana
Piccoli, Patricia Noemí
Burba, Jose Luis
Bottini, Ambrosio Rubén
author Pontin, Mariela Ana
author_facet Pontin, Mariela Ana
Piccoli, Patricia Noemí
Burba, Jose Luis
Bottini, Ambrosio Rubén
author_role author
author2 Piccoli, Patricia Noemí
Burba, Jose Luis
Bottini, Ambrosio Rubén
author2_role author
author
author
dc.subject.none.fl_str_mv Ecología de la planta
Allium Sativum
Ajo
Terpenoidos
Sclerotium Cepivorum
Propiedades Antimicósicas
Garlic
Terpenoids
Antifungal Properties
Terpenos
topic Ecología de la planta
Allium Sativum
Ajo
Terpenoidos
Sclerotium Cepivorum
Propiedades Antimicósicas
Garlic
Terpenoids
Antifungal Properties
Terpenos
dc.description.none.fl_txt_mv This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity
EEA La Consulta
Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2017-09-07T12:46:16Z
2017-09-07T12:46:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1161
http://ri.conicet.gov.ar/handle/11336/22405
http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub
0031-9422 (Print)
1873-3700 (Online)
https://doi.org/10.1016/j.phytochem.2015.02.003
url http://hdl.handle.net/20.500.12123/1161
http://ri.conicet.gov.ar/handle/11336/22405
http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub
https://doi.org/10.1016/j.phytochem.2015.02.003
identifier_str_mv 0031-9422 (Print)
1873-3700 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Mendoza (province)
dc.source.none.fl_str_mv Phytochemistry 115 :152-160. (July 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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