Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts

Autores
Huergo, María Ana Cristina; Pasquale, Miguel Ángel; González, Pedro Horacio; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The dynamics of two-dimensional (2D) radially spreading growth fronts of Vero cell colonies was investigated utilizing two types of colonies, namely type I starting from clusters with a small number of cells, which initially exhibited arbitrary-shaped rough growth fronts and progressively approached quasicircular ones as the cell population increased; and type II colonies, starting from a relatively large circular three-dimensional (3D) cell cluster. For large cell population colonies, the fractal dimension of the fronts was DF = 1.20 ± 0.05. For low cell populations, the mean colony radius increased exponentially with time, but for large ones the constant radial front velocity 0.20 ± 0.02 μm min−1 was reached. Colony spreading was accompanied by changes in both cell morphology and average size, and by the formation of very large cells, some of them multinuclear. Therefore the heterogeneity of colonies increased and local driving forces that set in began to influence the 2D growth front kinetics. The retardation effect related to the exponential to constant radial front velocity transition was assigned to a number of possible interferences including the cell duplication and 3D growth in the bulk of the colony. The dynamic scaling analysis of overhang-corrected rough colony fronts, after arc-radius coordinate system transformation, resulted in roughness exponent α = 0.50 ± 0.05 and growth exponent β = 0.32 ± 0.04, for arc lengths greater than 100 μm. This set of scaling exponents agreed with that predicted by the Kardar, Parisi, and Zhang continuous equation. For arc lengths shorter than 2–3 cell diameters, the value α = 0.85 ± 0.05 would be related to a cell front roughening caused by temporarily membrane deformations occasionally interfered by cell proliferation.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Facultad de Ciencias Médicas
Materia
Química
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/167168

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Dynamics and morphology characteristics of cell colonies with radially spreading growth frontsHuergo, María Ana CristinaPasquale, Miguel ÁngelGonzález, Pedro HoracioBolzán, Agustín EduardoArvia, Alejandro JorgeQuímicaThe dynamics of two-dimensional (2D) radially spreading growth fronts of Vero cell colonies was investigated utilizing two types of colonies, namely type I starting from clusters with a small number of cells, which initially exhibited arbitrary-shaped rough growth fronts and progressively approached quasicircular ones as the cell population increased; and type II colonies, starting from a relatively large circular three-dimensional (3D) cell cluster. For large cell population colonies, the fractal dimension of the fronts was DF = 1.20 ± 0.05. For low cell populations, the mean colony radius increased exponentially with time, but for large ones the constant radial front velocity 0.20 ± 0.02 μm min−1 was reached. Colony spreading was accompanied by changes in both cell morphology and average size, and by the formation of very large cells, some of them multinuclear. Therefore the heterogeneity of colonies increased and local driving forces that set in began to influence the 2D growth front kinetics. The retardation effect related to the exponential to constant radial front velocity transition was assigned to a number of possible interferences including the cell duplication and 3D growth in the bulk of the colony. The dynamic scaling analysis of overhang-corrected rough colony fronts, after arc-radius coordinate system transformation, resulted in roughness exponent α = 0.50 ± 0.05 and growth exponent β = 0.32 ± 0.04, for arc lengths greater than 100 μm. This set of scaling exponents agreed with that predicted by the Kardar, Parisi, and Zhang continuous equation. For arc lengths shorter than 2–3 cell diameters, the value α = 0.85 ± 0.05 would be related to a cell front roughening caused by temporarily membrane deformations occasionally interfered by cell proliferation.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasFacultad de Ciencias Médicas2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/167168enginfo:eu-repo/semantics/altIdentifier/issn/2470-0053info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.84.021917info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:44:30Zoai:sedici.unlp.edu.ar:10915/167168Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:44:31.026SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
title Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
spellingShingle Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
Huergo, María Ana Cristina
Química
title_short Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
title_full Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
title_fullStr Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
title_full_unstemmed Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
title_sort Dynamics and morphology characteristics of cell colonies with radially spreading growth fronts
dc.creator.none.fl_str_mv Huergo, María Ana Cristina
Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author Huergo, María Ana Cristina
author_facet Huergo, María Ana Cristina
Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author_role author
author2 Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
topic Química
dc.description.none.fl_txt_mv The dynamics of two-dimensional (2D) radially spreading growth fronts of Vero cell colonies was investigated utilizing two types of colonies, namely type I starting from clusters with a small number of cells, which initially exhibited arbitrary-shaped rough growth fronts and progressively approached quasicircular ones as the cell population increased; and type II colonies, starting from a relatively large circular three-dimensional (3D) cell cluster. For large cell population colonies, the fractal dimension of the fronts was DF = 1.20 ± 0.05. For low cell populations, the mean colony radius increased exponentially with time, but for large ones the constant radial front velocity 0.20 ± 0.02 μm min−1 was reached. Colony spreading was accompanied by changes in both cell morphology and average size, and by the formation of very large cells, some of them multinuclear. Therefore the heterogeneity of colonies increased and local driving forces that set in began to influence the 2D growth front kinetics. The retardation effect related to the exponential to constant radial front velocity transition was assigned to a number of possible interferences including the cell duplication and 3D growth in the bulk of the colony. The dynamic scaling analysis of overhang-corrected rough colony fronts, after arc-radius coordinate system transformation, resulted in roughness exponent α = 0.50 ± 0.05 and growth exponent β = 0.32 ± 0.04, for arc lengths greater than 100 μm. This set of scaling exponents agreed with that predicted by the Kardar, Parisi, and Zhang continuous equation. For arc lengths shorter than 2–3 cell diameters, the value α = 0.85 ± 0.05 would be related to a cell front roughening caused by temporarily membrane deformations occasionally interfered by cell proliferation.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Facultad de Ciencias Médicas
description The dynamics of two-dimensional (2D) radially spreading growth fronts of Vero cell colonies was investigated utilizing two types of colonies, namely type I starting from clusters with a small number of cells, which initially exhibited arbitrary-shaped rough growth fronts and progressively approached quasicircular ones as the cell population increased; and type II colonies, starting from a relatively large circular three-dimensional (3D) cell cluster. For large cell population colonies, the fractal dimension of the fronts was DF = 1.20 ± 0.05. For low cell populations, the mean colony radius increased exponentially with time, but for large ones the constant radial front velocity 0.20 ± 0.02 μm min−1 was reached. Colony spreading was accompanied by changes in both cell morphology and average size, and by the formation of very large cells, some of them multinuclear. Therefore the heterogeneity of colonies increased and local driving forces that set in began to influence the 2D growth front kinetics. The retardation effect related to the exponential to constant radial front velocity transition was assigned to a number of possible interferences including the cell duplication and 3D growth in the bulk of the colony. The dynamic scaling analysis of overhang-corrected rough colony fronts, after arc-radius coordinate system transformation, resulted in roughness exponent α = 0.50 ± 0.05 and growth exponent β = 0.32 ± 0.04, for arc lengths greater than 100 μm. This set of scaling exponents agreed with that predicted by the Kardar, Parisi, and Zhang continuous equation. For arc lengths shorter than 2–3 cell diameters, the value α = 0.85 ± 0.05 would be related to a cell front roughening caused by temporarily membrane deformations occasionally interfered by cell proliferation.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/167168
url http://sedici.unlp.edu.ar/handle/10915/167168
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2470-0053
info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.84.021917
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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reponame_str SEDICI (UNLP)
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