Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media

Autores
Muzzio, Nicolás Eduardo; Carballido, Marcos Daniel; Pasquale, Miguel Ángel; González, Pedro Horacio; Azzaroni, Omar; Arvia, Alejandro Jorge
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (cEGF) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations. For this purpose, HeLa cell colonies that remain confluent along the whole expansion process with initial quasi-radial geometry and different initial cell populations, as well as colonies with initial quasi-linear geometry and large cell population, are employed. Cell size and morphology as well as its adhesive characteristics depend on cEGF. Quasi-radial colonies (QRC) expansion kinetics in EGF-containing medium exhibits a complex behavior. Namely, at the first stages of growth, the average QRC radius evolution can be described by a t1/2 diffusion term coupled with exponential growth kinetics up to a critical time, and afterwards a growth regime approaching constant velocity. The extension of each regime depends on cEGF and colony history. In the presence of EGF, the initial expansion of quasi-linear colonies (QLCs) also exhibits morphological changes at both the cell and the colony levels. In these cases, the cell density at the colony border region becomes smaller than in the absence of EGF and consequently, the extension of the effective rim where cell duplication and motility contribute to the colony expansion increases. QLC front displacement velocity increases with cEGF up to a maximum value in the 2–10ng ml−1 range. Individual cell velocity is increased by EGF, and an enhancement in both the persistence and the ballistic characteristics of cell trajectories can be distinguished. For an intermediate cEGF, collective cell displacements contribute to the roughening of the colony contours. This global dynamics becomes compatible with the standard Kardar–Parisi–Zhang growth model, although a faster colony roughness saturation in EGFcontaining medium than in the control medium is observed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Ciencias Exactas
Química
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127398

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network_name_str SEDICI (UNLP)
spelling Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented mediaMuzzio, Nicolás EduardoCarballido, Marcos DanielPasquale, Miguel ÁngelGonzález, Pedro HoracioAzzaroni, OmarArvia, Alejandro JorgeCiencias ExactasQuímicaEpidermal growth factorHeLa cellColony kineticsDynamic scalingCell transport mechanismThe epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (c<sub>EGF</sub>) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations. For this purpose, HeLa cell colonies that remain confluent along the whole expansion process with initial quasi-radial geometry and different initial cell populations, as well as colonies with initial quasi-linear geometry and large cell population, are employed. Cell size and morphology as well as its adhesive characteristics depend on c<sub>EGF</sub>. Quasi-radial colonies (QRC) expansion kinetics in EGF-containing medium exhibits a complex behavior. Namely, at the first stages of growth, the average QRC radius evolution can be described by a t<sup>1/2</sup> diffusion term coupled with exponential growth kinetics up to a critical time, and afterwards a growth regime approaching constant velocity. The extension of each regime depends on c<sub>EGF</sub> and colony history. In the presence of EGF, the initial expansion of quasi-linear colonies (QLCs) also exhibits morphological changes at both the cell and the colony levels. In these cases, the cell density at the colony border region becomes smaller than in the absence of EGF and consequently, the extension of the effective rim where cell duplication and motility contribute to the colony expansion increases. QLC front displacement velocity increases with c<sub>EGF</sub> up to a maximum value in the 2–10ng ml<sup>−1</sup> range. Individual cell velocity is increased by EGF, and an enhancement in both the persistence and the ballistic characteristics of cell trajectories can be distinguished. For an intermediate c<sub>EGF</sub>, collective cell displacements contribute to the roughening of the colony contours. This global dynamics becomes compatible with the standard Kardar–Parisi–Zhang growth model, although a faster colony roughness saturation in EGFcontaining medium than in the control medium is observed.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2018-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/127398enginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1478-3975/aabc2finfo:eu-repo/semantics/altIdentifier/issn/1478-3975info:eu-repo/semantics/altIdentifier/doi/10.1088/1478-3975/aabc2finfo:eu-repo/semantics/reference/hdl/10915/97244info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:47Zoai:sedici.unlp.edu.ar:10915/127398Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:48.142SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
spellingShingle Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
Muzzio, Nicolás Eduardo
Ciencias Exactas
Química
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
title_short Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_full Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_fullStr Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_full_unstemmed Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
title_sort Morphology and dynamics of tumor cell colonies propagating in epidermal growth factor supplemented media
dc.creator.none.fl_str_mv Muzzio, Nicolás Eduardo
Carballido, Marcos Daniel
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
author Muzzio, Nicolás Eduardo
author_facet Muzzio, Nicolás Eduardo
Carballido, Marcos Daniel
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
author_role author
author2 Carballido, Marcos Daniel
Pasquale, Miguel Ángel
González, Pedro Horacio
Azzaroni, Omar
Arvia, Alejandro Jorge
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
topic Ciencias Exactas
Química
Epidermal growth factor
HeLa cell
Colony kinetics
Dynamic scaling
Cell transport mechanism
dc.description.none.fl_txt_mv The epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (c<sub>EGF</sub>) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations. For this purpose, HeLa cell colonies that remain confluent along the whole expansion process with initial quasi-radial geometry and different initial cell populations, as well as colonies with initial quasi-linear geometry and large cell population, are employed. Cell size and morphology as well as its adhesive characteristics depend on c<sub>EGF</sub>. Quasi-radial colonies (QRC) expansion kinetics in EGF-containing medium exhibits a complex behavior. Namely, at the first stages of growth, the average QRC radius evolution can be described by a t<sup>1/2</sup> diffusion term coupled with exponential growth kinetics up to a critical time, and afterwards a growth regime approaching constant velocity. The extension of each regime depends on c<sub>EGF</sub> and colony history. In the presence of EGF, the initial expansion of quasi-linear colonies (QLCs) also exhibits morphological changes at both the cell and the colony levels. In these cases, the cell density at the colony border region becomes smaller than in the absence of EGF and consequently, the extension of the effective rim where cell duplication and motility contribute to the colony expansion increases. QLC front displacement velocity increases with c<sub>EGF</sub> up to a maximum value in the 2–10ng ml<sup>−1</sup> range. Individual cell velocity is increased by EGF, and an enhancement in both the persistence and the ballistic characteristics of cell trajectories can be distinguished. For an intermediate c<sub>EGF</sub>, collective cell displacements contribute to the roughening of the colony contours. This global dynamics becomes compatible with the standard Kardar–Parisi–Zhang growth model, although a faster colony roughness saturation in EGFcontaining medium than in the control medium is observed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description The epidermal growth factor (EGF) plays a key role in physiological and pathological processes. This work reports on the influence of EGF concentration (c<sub>EGF</sub>) on the modulation of individual cell phenotype and cell colony kinetics with the aim of perturbing the colony front roughness fluctuations. For this purpose, HeLa cell colonies that remain confluent along the whole expansion process with initial quasi-radial geometry and different initial cell populations, as well as colonies with initial quasi-linear geometry and large cell population, are employed. Cell size and morphology as well as its adhesive characteristics depend on c<sub>EGF</sub>. Quasi-radial colonies (QRC) expansion kinetics in EGF-containing medium exhibits a complex behavior. Namely, at the first stages of growth, the average QRC radius evolution can be described by a t<sup>1/2</sup> diffusion term coupled with exponential growth kinetics up to a critical time, and afterwards a growth regime approaching constant velocity. The extension of each regime depends on c<sub>EGF</sub> and colony history. In the presence of EGF, the initial expansion of quasi-linear colonies (QLCs) also exhibits morphological changes at both the cell and the colony levels. In these cases, the cell density at the colony border region becomes smaller than in the absence of EGF and consequently, the extension of the effective rim where cell duplication and motility contribute to the colony expansion increases. QLC front displacement velocity increases with c<sub>EGF</sub> up to a maximum value in the 2–10ng ml<sup>−1</sup> range. Individual cell velocity is increased by EGF, and an enhancement in both the persistence and the ballistic characteristics of cell trajectories can be distinguished. For an intermediate c<sub>EGF</sub>, collective cell displacements contribute to the roughening of the colony contours. This global dynamics becomes compatible with the standard Kardar–Parisi–Zhang growth model, although a faster colony roughness saturation in EGFcontaining medium than in the control medium is observed.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127398
url http://sedici.unlp.edu.ar/handle/10915/127398
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1478-3975/aabc2f
info:eu-repo/semantics/altIdentifier/issn/1478-3975
info:eu-repo/semantics/altIdentifier/doi/10.1088/1478-3975/aabc2f
info:eu-repo/semantics/reference/hdl/10915/97244
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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