Growth dynamics of cancer cell colonies and their comparison with noncancerous cells

Autores
Huergo, María Ana Cristina; Pasquale, Miguel Angel; González, P. H.; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
Fil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: González, P. H.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Bolzán, Agustín Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Arvia, Alejandro Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
DYNAMIC SCALING
HELA CELLS
COLONY GROWTH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268972
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268972
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network_name_str CONICET Digital (CONICET)
spelling Growth dynamics of cancer cell colonies and their comparison with noncancerous cellsHuergo, María Ana CristinaPasquale, Miguel AngelGonzález, P. H.Bolzán, Agustín EduardoArvia, Alejandro JorgeDYNAMIC SCALINGHELA CELLSCOLONY GROWTHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.Fil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: González, P. H.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Bolzán, Agustín Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Arvia, Alejandro Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaAmerican Physical Society2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268972Huergo, María Ana Cristina; Pasquale, Miguel Angel; González, P. H.; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge; Growth dynamics of cancer cell colonies and their comparison with noncancerous cells; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 85; 1; 2-2012; 1-91539-3755CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pre.aps.org/abstract/PRE/v85/i1/e011918info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.85.011918info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:07Zoai:ri.conicet.gov.ar:11336/268972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:08.059CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
spellingShingle Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
Huergo, María Ana Cristina
DYNAMIC SCALING
HELA CELLS
COLONY GROWTH
title_short Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_full Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_fullStr Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_full_unstemmed Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_sort Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
dc.creator.none.fl_str_mv Huergo, María Ana Cristina
Pasquale, Miguel Angel
González, P. H.
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author Huergo, María Ana Cristina
author_facet Huergo, María Ana Cristina
Pasquale, Miguel Angel
González, P. H.
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author_role author
author2 Pasquale, Miguel Angel
González, P. H.
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv DYNAMIC SCALING
HELA CELLS
COLONY GROWTH
topic DYNAMIC SCALING
HELA CELLS
COLONY GROWTH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
Fil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: González, P. H.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Bolzán, Agustín Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Arvia, Alejandro Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268972
Huergo, María Ana Cristina; Pasquale, Miguel Angel; González, P. H.; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge; Growth dynamics of cancer cell colonies and their comparison with noncancerous cells; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 85; 1; 2-2012; 1-9
1539-3755
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268972
identifier_str_mv Huergo, María Ana Cristina; Pasquale, Miguel Angel; González, P. H.; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge; Growth dynamics of cancer cell colonies and their comparison with noncancerous cells; American Physical Society; Physical Review E: Statistical, Nonlinear and Soft Matter Physics; 85; 1; 2-2012; 1-9
1539-3755
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pre.aps.org/abstract/PRE/v85/i1/e011918
info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.85.011918
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physical Society
publisher.none.fl_str_mv American Physical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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