Growth dynamics of cancer cell colonies and their comparison with noncancerous cells

Autores
Huergo, María Ana Cristina; Pasquale, Miguel Ángel; González, Pedro Horacio; Bolzán, Agustín Eduardo; Arvia, Alejandro Jorge
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Facultad de Ciencias Médicas
Materia
Química
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/113638

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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Growth dynamics of cancer cell colonies and their comparison with noncancerous cellsHuergo, María Ana CristinaPasquale, Miguel ÁngelGonzález, Pedro HoracioBolzán, Agustín EduardoArvia, Alejandro JorgeQuímicaThe two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasFacultad de Ciencias Médicas2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/113638enginfo:eu-repo/semantics/altIdentifier/issn/1539-3755info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.85.011918info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:26:25Zoai:sedici.unlp.edu.ar:10915/113638Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:26:25.742SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
spellingShingle Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
Huergo, María Ana Cristina
Química
title_short Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_full Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_fullStr Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_full_unstemmed Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
title_sort Growth dynamics of cancer cell colonies and their comparison with noncancerous cells
dc.creator.none.fl_str_mv Huergo, María Ana Cristina
Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author Huergo, María Ana Cristina
author_facet Huergo, María Ana Cristina
Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author_role author
author2 Pasquale, Miguel Ángel
González, Pedro Horacio
Bolzán, Agustín Eduardo
Arvia, Alejandro Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
topic Química
dc.description.none.fl_txt_mv The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Facultad de Ciencias Médicas
description The two-dimensional (2D) growth dynamics of HeLa (cervix cancer) cell colonies was studied following both their growth front and the pattern morphology evolutions utilizing large population colonies exhibiting linearly and radially spreading fronts. In both cases, the colony profile fractal dimension was df = 1.20 ± 0.05 and the growth fronts displaced at the constant velocity 0.90 ± 0.05 μm min−1. Colonies showed changes in both cell morphology and average size. As time increased, the formation of large cells at the colony front was observed. Accordingly, the heterogeneity of the colony increased and local driving forces that set in began to influence the dynamics of the colony front. The dynamic scaling analysis of rough colony fronts resulted in a roughness exponent α = 0.50 ± 0.05, a growth exponent β = 0.32 ± 0.04, and a dynamic exponent z = 1.5 ± 0.2. The validity of this set of scaling exponents extended from a lower cutoff lc ≈ 60 μm upward, and the exponents agreed with those predicted by the standard Kardar-Parisi-Zhang continuous equation. HeLa data were compared with those previously reported for Vero cell colonies. The value of df and the Kardar-Parisi-Zhang-type 2D front growth dynamics were similar for colonies of both cell lines. This indicates that the cell colony growth dynamics is independent of the genetic background and the tumorigenic nature of the cells. However, one can distinguish some differences between both cell lines during the growth of colonies that may result from specific cooperative effects and the nature of each biosystem.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/113638
url http://sedici.unlp.edu.ar/handle/10915/113638
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1539-3755
info:eu-repo/semantics/altIdentifier/doi/10.1103/PhysRevE.85.011918
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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