The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
- Autores
- Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; Torres, Alfredo G.; Rivas, Marta; Tóth, István
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Instituto de Genética Veterinaria - Materia
-
Biología
Ciencias Veterinarias
Escherichia coli O157
Atypical e. coli
Ppathogenic E. col
Long polar fimbriae - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104707
Ver los metadatos del registro completo
id |
SEDICI_da6ac77157b43f62e058353b3fcdc74d |
---|---|
oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/104707 |
network_acronym_str |
SEDICI |
repository_id_str |
1329 |
network_name_str |
SEDICI (UNLP) |
spelling |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strainsSváb, DomonkosGalli, LucíaHorváth, BalázsMaróti, GergelyDobrindt, UlrichTorres, Alfredo G.Rivas, MartaTóth, IstvánBiologíaCiencias VeterinariasEscherichia coli O157Atypical e. coliPpathogenic E. colLong polar fimbriaeLong polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.Instituto de Genética Veterinaria2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-7http://sedici.unlp.edu.ar/handle/10915/104707enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/11603info:eu-repo/semantics/altIdentifier/issn/2049-632Xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038info:eu-repo/semantics/altIdentifier/hdl/11336/11603info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:42Zoai:sedici.unlp.edu.ar:10915/104707Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:43.071SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
spellingShingle |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains Sváb, Domonkos Biología Ciencias Veterinarias Escherichia coli O157 Atypical e. coli Ppathogenic E. col Long polar fimbriae |
title_short |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_full |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_fullStr |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_full_unstemmed |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_sort |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
dc.creator.none.fl_str_mv |
Sváb, Domonkos Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author |
Sváb, Domonkos |
author_facet |
Sváb, Domonkos Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author_role |
author |
author2 |
Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Biología Ciencias Veterinarias Escherichia coli O157 Atypical e. coli Ppathogenic E. col Long polar fimbriae |
topic |
Biología Ciencias Veterinarias Escherichia coli O157 Atypical e. coli Ppathogenic E. col Long polar fimbriae |
dc.description.none.fl_txt_mv |
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins. Instituto de Genética Veterinaria |
description |
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/104707 |
url |
http://sedici.unlp.edu.ar/handle/10915/104707 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/11603 info:eu-repo/semantics/altIdentifier/issn/2049-632X info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038 info:eu-repo/semantics/altIdentifier/hdl/11336/11603 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 1-7 |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
collection |
SEDICI (UNLP) |
instname_str |
Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
_version_ |
1844616102987956224 |
score |
13.070432 |