The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains

Autores
Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; Torres, Alfredo G.; Rivas, Marta; Tóth, István
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Instituto de Genética Veterinaria
Materia
Biología
Ciencias Veterinarias
Escherichia coli O157
Atypical e. coli
Ppathogenic E. col
Long polar fimbriae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104707
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/104707
network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strainsSváb, DomonkosGalli, LucíaHorváth, BalázsMaróti, GergelyDobrindt, UlrichTorres, Alfredo G.Rivas, MartaTóth, IstvánBiologíaCiencias VeterinariasEscherichia coli O157Atypical e. coliPpathogenic E. colLong polar fimbriaeLong polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.Instituto de Genética Veterinaria2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-7http://sedici.unlp.edu.ar/handle/10915/104707enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/11603info:eu-repo/semantics/altIdentifier/issn/2049-632Xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038info:eu-repo/semantics/altIdentifier/hdl/11336/11603info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:03:31Zoai:sedici.unlp.edu.ar:10915/104707Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:03:32.272SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
spellingShingle The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
Sváb, Domonkos
Biología
Ciencias Veterinarias
Escherichia coli O157
Atypical e. coli
Ppathogenic E. col
Long polar fimbriae
title_short The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_full The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_fullStr The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_full_unstemmed The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_sort The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
dc.creator.none.fl_str_mv Sváb, Domonkos
Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author Sváb, Domonkos
author_facet Sváb, Domonkos
Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author_role author
author2 Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Veterinarias
Escherichia coli O157
Atypical e. coli
Ppathogenic E. col
Long polar fimbriae
topic Biología
Ciencias Veterinarias
Escherichia coli O157
Atypical e. coli
Ppathogenic E. col
Long polar fimbriae
dc.description.none.fl_txt_mv Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Instituto de Genética Veterinaria
description Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104707
url http://sedici.unlp.edu.ar/handle/10915/104707
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/11603
info:eu-repo/semantics/altIdentifier/issn/2049-632X
info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038
info:eu-repo/semantics/altIdentifier/hdl/11336/11603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1-7
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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