The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains

Autores
Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; Torres, Alfredo G.; Rivas, Marta; Tóth, István
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Fil: Sváb, Domonkos. Hungarian Academy of Sciences; Hungría
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Dobrindt, Ulrich. University of Münster; Alemania
Fil: Torres, Alfredo G.. University Of Texas; Estados Unidos
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Tóth, István . Hungarian Academy of Sciences; Hungría
Materia
ESCHERICHIA COLI
O157:H43
ATYPICAL E. COLI
LPF
O136:H12
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11603
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11603
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strainsSváb, DomonkosGalli, LucíaHorváth, BalázsMaróti, GergelyDobrindt, UlrichTorres, Alfredo G.Rivas, MartaTóth, István ESCHERICHIA COLIO157:H43ATYPICAL E. COLILPFO136:H12https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.Fil: Sváb, Domonkos. Hungarian Academy of Sciences; HungríaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; HungríaFil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; HungríaFil: Dobrindt, Ulrich. University of Münster; AlemaniaFil: Torres, Alfredo G.. University Of Texas; Estados UnidosFil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Tóth, István . Hungarian Academy of Sciences; HungríaOxford University Press2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11603Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-72049-632Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038info:eu-repo/semantics/altIdentifier/url/http://femspd.oxfordjournals.org/content/68/1/1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821771/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:08:13Zoai:ri.conicet.gov.ar:11336/11603instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:08:13.642CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
spellingShingle The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
Sváb, Domonkos
ESCHERICHIA COLI
O157:H43
ATYPICAL E. COLI
LPF
O136:H12
title_short The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_full The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_fullStr The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_full_unstemmed The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
title_sort The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
dc.creator.none.fl_str_mv Sváb, Domonkos
Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author Sváb, Domonkos
author_facet Sváb, Domonkos
Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author_role author
author2 Galli, Lucía
Horváth, Balázs
Maróti, Gergely
Dobrindt, Ulrich
Torres, Alfredo G.
Rivas, Marta
Tóth, István
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESCHERICHIA COLI
O157:H43
ATYPICAL E. COLI
LPF
O136:H12
topic ESCHERICHIA COLI
O157:H43
ATYPICAL E. COLI
LPF
O136:H12
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Fil: Sváb, Domonkos. Hungarian Academy of Sciences; Hungría
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Dobrindt, Ulrich. University of Münster; Alemania
Fil: Torres, Alfredo G.. University Of Texas; Estados Unidos
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Tóth, István . Hungarian Academy of Sciences; Hungría
description Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11603
Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-7
2049-632X
url http://hdl.handle.net/11336/11603
identifier_str_mv Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-7
2049-632X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038
info:eu-repo/semantics/altIdentifier/url/http://femspd.oxfordjournals.org/content/68/1/1
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821771/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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