The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains
- Autores
- Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; Torres, Alfredo G.; Rivas, Marta; Tóth, István
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.
Fil: Sváb, Domonkos. Hungarian Academy of Sciences; Hungría
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría
Fil: Dobrindt, Ulrich. University of Münster; Alemania
Fil: Torres, Alfredo G.. University Of Texas; Estados Unidos
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Tóth, István . Hungarian Academy of Sciences; Hungría - Materia
-
ESCHERICHIA COLI
O157:H43
ATYPICAL E. COLI
LPF
O136:H12 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/11603
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strainsSváb, DomonkosGalli, LucíaHorváth, BalázsMaróti, GergelyDobrindt, UlrichTorres, Alfredo G.Rivas, MartaTóth, István ESCHERICHIA COLIO157:H43ATYPICAL E. COLILPFO136:H12https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins.Fil: Sváb, Domonkos. Hungarian Academy of Sciences; HungríaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; HungríaFil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; HungríaFil: Dobrindt, Ulrich. University of Münster; AlemaniaFil: Torres, Alfredo G.. University Of Texas; Estados UnidosFil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Tóth, István . Hungarian Academy of Sciences; HungríaOxford University Press2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11603Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-72049-632Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038info:eu-repo/semantics/altIdentifier/url/http://femspd.oxfordjournals.org/content/68/1/1info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821771/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:47Zoai:ri.conicet.gov.ar:11336/11603instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:47.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
spellingShingle |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains Sváb, Domonkos ESCHERICHIA COLI O157:H43 ATYPICAL E. COLI LPF O136:H12 |
title_short |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_full |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_fullStr |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_full_unstemmed |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
title_sort |
The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains |
dc.creator.none.fl_str_mv |
Sváb, Domonkos Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author |
Sváb, Domonkos |
author_facet |
Sváb, Domonkos Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author_role |
author |
author2 |
Galli, Lucía Horváth, Balázs Maróti, Gergely Dobrindt, Ulrich Torres, Alfredo G. Rivas, Marta Tóth, István |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
ESCHERICHIA COLI O157:H43 ATYPICAL E. COLI LPF O136:H12 |
topic |
ESCHERICHIA COLI O157:H43 ATYPICAL E. COLI LPF O136:H12 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins. Fil: Sváb, Domonkos. Hungarian Academy of Sciences; Hungría Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina Fil: Horváth, Balázs. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría Fil: Maróti, Gergely. Hungarian Academy of Sciences; Hungría. Bay Zoltán Nonprofit Research; Hungría Fil: Dobrindt, Ulrich. University of Münster; Alemania Fil: Torres, Alfredo G.. University Of Texas; Estados Unidos Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina Fil: Tóth, István . Hungarian Academy of Sciences; Hungría |
description |
Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative E. coli O157:H43 strain of bovine origin, and also sequenced the lpf2-1 operon of 6 additional atypical O157 bovine Escherichia coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 Escherichia coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci is highly conserved in E. coli isolates, however, its role in adherence might be masked by other uncharacterized adhesins. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/11603 Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-7 2049-632X |
url |
http://hdl.handle.net/11336/11603 |
identifier_str_mv |
Sváb, Domonkos; Galli, Lucía; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; et al.; The long polar fimbriae (lpf) operon and its flanking regions in atypical bovine Escherichia coli O157:H43 and STEC O136:H12 strains; Oxford University Press; Pathogens and Disease; 68; 1; 4-2013; 1-7 2049-632X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12038 info:eu-repo/semantics/altIdentifier/url/http://femspd.oxfordjournals.org/content/68/1/1 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821771/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613259076829184 |
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13.070432 |