Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat

Autores
Aiello, Ernesto Alejandro; Grassi de Gende, Ángela
Año de publicación
1993
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strips of rat ventricle were treated with EGTA (5 mM) for 24 h at 4°C and used to perform isotopical measurements of transport and release of Ca2+ in the SR in situ.45Ca accumulated by these preparations showed dependence on time of incubation until it reached saturation after 30 min. Rate and capacity of Ca2+ accumulation were calculated in 0.075 nmol mg ww−1 min−1 and 0.402 nmol mg ww−1, respectively. These values were increased by a factor of 2.6 and 8.6 when K oxalate was present in the incubation media as could be expected for Ca2+ transported by SR. Ca2+ release was assayed on45Ca desaturation curves at three free Ca2+ concentrations: 0.3, 1 and 10 μM. Significant increases in the velocity of Ca2+ efflux and net release of Ca2+ were induced only by 1 μM free Ca2+, and the Ca2+ release could be inhibited by 75% when 50 μM of ruthenium red was included in the washout solution. These results are in agreement with those obtained in assessing the SR function by mechanical measurements in skinned cardiac cells or by biochemical determinations in isolated cardiac SR vesicles. In spite of the fact that the resolution time is not as high as that required for the physiological handling of Ca2+ by SR, this methodology looks promising for approaching the SR function in cardiac pathologies as well as the effects of drugs on transport and release of Ca2+ by cardiac SR.
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Cardiac sarcoplasmic reticulum
Chemically skinned cardiac cells
4SCa transport and release
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131211

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network_name_str SEDICI (UNLP)
spelling Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the ratAiello, Ernesto AlejandroGrassi de Gende, ÁngelaCiencias MédicasCardiac sarcoplasmic reticulumChemically skinned cardiac cells4SCa transport and releaseStrips of rat ventricle were treated with EGTA (5 mM) for 24 h at 4°C and used to perform isotopical measurements of transport and release of Ca2+ in the SR in situ.45Ca accumulated by these preparations showed dependence on time of incubation until it reached saturation after 30 min. Rate and capacity of Ca2+ accumulation were calculated in 0.075 nmol mg ww−1 min−1 and 0.402 nmol mg ww−1, respectively. These values were increased by a factor of 2.6 and 8.6 when K oxalate was present in the incubation media as could be expected for Ca2+ transported by SR. Ca2+ release was assayed on45Ca desaturation curves at three free Ca2+ concentrations: 0.3, 1 and 10 μM. Significant increases in the velocity of Ca2+ efflux and net release of Ca2+ were induced only by 1 μM free Ca2+, and the Ca2+ release could be inhibited by 75% when 50 μM of ruthenium red was included in the washout solution. These results are in agreement with those obtained in assessing the SR function by mechanical measurements in skinned cardiac cells or by biochemical determinations in isolated cardiac SR vesicles. In spite of the fact that the resolution time is not as high as that required for the physiological handling of Ca2+ by SR, this methodology looks promising for approaching the SR function in cardiac pathologies as well as the effects of drugs on transport and release of Ca2+ by cardiac SR.Centro de Investigaciones Cardiovasculares1993info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf33-41http://sedici.unlp.edu.ar/handle/10915/131211enginfo:eu-repo/semantics/altIdentifier/issn/0300-8428info:eu-repo/semantics/altIdentifier/issn/1435-1803info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00788528info:eu-repo/semantics/altIdentifier/pmid/7682409info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:47Zoai:sedici.unlp.edu.ar:10915/131211Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:47.31SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
title Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
spellingShingle Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
Aiello, Ernesto Alejandro
Ciencias Médicas
Cardiac sarcoplasmic reticulum
Chemically skinned cardiac cells
4SCa transport and release
title_short Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
title_full Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
title_fullStr Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
title_full_unstemmed Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
title_sort Transport and release of calcium by sarcoplasmic reticulum in chemically skinned ventricular muscle of the rat
dc.creator.none.fl_str_mv Aiello, Ernesto Alejandro
Grassi de Gende, Ángela
author Aiello, Ernesto Alejandro
author_facet Aiello, Ernesto Alejandro
Grassi de Gende, Ángela
author_role author
author2 Grassi de Gende, Ángela
author2_role author
dc.subject.none.fl_str_mv Ciencias Médicas
Cardiac sarcoplasmic reticulum
Chemically skinned cardiac cells
4SCa transport and release
topic Ciencias Médicas
Cardiac sarcoplasmic reticulum
Chemically skinned cardiac cells
4SCa transport and release
dc.description.none.fl_txt_mv Strips of rat ventricle were treated with EGTA (5 mM) for 24 h at 4°C and used to perform isotopical measurements of transport and release of Ca2+ in the SR in situ.45Ca accumulated by these preparations showed dependence on time of incubation until it reached saturation after 30 min. Rate and capacity of Ca2+ accumulation were calculated in 0.075 nmol mg ww−1 min−1 and 0.402 nmol mg ww−1, respectively. These values were increased by a factor of 2.6 and 8.6 when K oxalate was present in the incubation media as could be expected for Ca2+ transported by SR. Ca2+ release was assayed on45Ca desaturation curves at three free Ca2+ concentrations: 0.3, 1 and 10 μM. Significant increases in the velocity of Ca2+ efflux and net release of Ca2+ were induced only by 1 μM free Ca2+, and the Ca2+ release could be inhibited by 75% when 50 μM of ruthenium red was included in the washout solution. These results are in agreement with those obtained in assessing the SR function by mechanical measurements in skinned cardiac cells or by biochemical determinations in isolated cardiac SR vesicles. In spite of the fact that the resolution time is not as high as that required for the physiological handling of Ca2+ by SR, this methodology looks promising for approaching the SR function in cardiac pathologies as well as the effects of drugs on transport and release of Ca2+ by cardiac SR.
Centro de Investigaciones Cardiovasculares
description Strips of rat ventricle were treated with EGTA (5 mM) for 24 h at 4°C and used to perform isotopical measurements of transport and release of Ca2+ in the SR in situ.45Ca accumulated by these preparations showed dependence on time of incubation until it reached saturation after 30 min. Rate and capacity of Ca2+ accumulation were calculated in 0.075 nmol mg ww−1 min−1 and 0.402 nmol mg ww−1, respectively. These values were increased by a factor of 2.6 and 8.6 when K oxalate was present in the incubation media as could be expected for Ca2+ transported by SR. Ca2+ release was assayed on45Ca desaturation curves at three free Ca2+ concentrations: 0.3, 1 and 10 μM. Significant increases in the velocity of Ca2+ efflux and net release of Ca2+ were induced only by 1 μM free Ca2+, and the Ca2+ release could be inhibited by 75% when 50 μM of ruthenium red was included in the washout solution. These results are in agreement with those obtained in assessing the SR function by mechanical measurements in skinned cardiac cells or by biochemical determinations in isolated cardiac SR vesicles. In spite of the fact that the resolution time is not as high as that required for the physiological handling of Ca2+ by SR, this methodology looks promising for approaching the SR function in cardiac pathologies as well as the effects of drugs on transport and release of Ca2+ by cardiac SR.
publishDate 1993
dc.date.none.fl_str_mv 1993
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131211
url http://sedici.unlp.edu.ar/handle/10915/131211
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1435-1803
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00788528
info:eu-repo/semantics/altIdentifier/pmid/7682409
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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