Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats

Autores
Cuneo, M. E.; Grassi de Gende, Ángela Ofelia
Año de publicación
1988
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168±4 and 202±6 mm Hg, respectively). The amount of SR protein (mg/g of left ventricle) was higher when obtained from hypertrophic ventricles of both hypertensive groups than from ventricles of the control group. The result agreed with the enhanced Ca2+ uptake exhibited by left ventricular homogenates of the hypertensive groups. Consequently, Ca2+ uptake in SR microsomes isolated per gram of left ventricle (nmol Ca2+/g muscle) was 51.62±10.06 and 64.99±12.84 in mildly and severely hypertensive groups vs. 17.37±5.79 in the control group (P<0.05). The SR microsomes obtained from ventricles of hypertensive rats showed an enhanced Ca2+ activated ATPase activity that was not accompanied by increased Ca2+ uptake at saturating calcium concentrations, but by increased affinity for calcium (K'app of 1.09±0.28 and 2.67±0.16 μM in SR microsomes of hypertrophic and control ventricles respectively; P<0.05). The rats of calcium loss, measured in SR vesicles passively loaded with45Ca, were similar when assayed in SR obtained from ventricles of both hypertensive and normal rats. These results enable us to suggest that in hearts of rats presenting spontaneous hypertension, the function of the SR system could account for a normal handling of cytosolic calcium. They might support the absence of mechanical alterations described in hearts of young rats of the SHR strain.
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
spontaneously hypertensive rats
cardiac hypertrophy
sarcoplasmic reticulum
Ca 2+ uptake
Ca 2+ ATPase activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141080
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/141080
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network_name_str SEDICI (UNLP)
spelling Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive ratsCuneo, M. E.Grassi de Gende, Ángela OfeliaCiencias Médicasspontaneously hypertensive ratscardiac hypertrophysarcoplasmic reticulumCa 2+ uptakeCa 2+ ATPase activitySarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168±4 and 202±6 mm Hg, respectively). The amount of SR protein (mg/g of left ventricle) was higher when obtained from hypertrophic ventricles of both hypertensive groups than from ventricles of the control group. The result agreed with the enhanced Ca2+ uptake exhibited by left ventricular homogenates of the hypertensive groups. Consequently, Ca2+ uptake in SR microsomes isolated per gram of left ventricle (nmol Ca2+/g muscle) was 51.62±10.06 and 64.99±12.84 in mildly and severely hypertensive groups vs. 17.37±5.79 in the control group (P<0.05). The SR microsomes obtained from ventricles of hypertensive rats showed an enhanced Ca2+ activated ATPase activity that was not accompanied by increased Ca2+ uptake at saturating calcium concentrations, but by increased affinity for calcium (K'app of 1.09±0.28 and 2.67±0.16 μM in SR microsomes of hypertrophic and control ventricles respectively; P<0.05). The rats of calcium loss, measured in SR vesicles passively loaded with45Ca, were similar when assayed in SR obtained from ventricles of both hypertensive and normal rats. These results enable us to suggest that in hearts of rats presenting spontaneous hypertension, the function of the SR system could account for a normal handling of cytosolic calcium. They might support the absence of mechanical alterations described in hearts of young rats of the SHR strain.Centro de Investigaciones Cardiovasculares1988info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf286-295http://sedici.unlp.edu.ar/handle/10915/141080enginfo:eu-repo/semantics/altIdentifier/issn/0300-8428info:eu-repo/semantics/altIdentifier/issn/1435-1803info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01907362info:eu-repo/semantics/altIdentifier/pmid/2843160info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:07Zoai:sedici.unlp.edu.ar:10915/141080Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:08.191SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
title Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
spellingShingle Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
Cuneo, M. E.
Ciencias Médicas
spontaneously hypertensive rats
cardiac hypertrophy
sarcoplasmic reticulum
Ca 2+ uptake
Ca 2+ ATPase activity
title_short Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
title_full Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
title_fullStr Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
title_full_unstemmed Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
title_sort Cardiac sarcoplasmic reticulum characteristics in hypertrophic hearts from spontaneously hypertensive rats
dc.creator.none.fl_str_mv Cuneo, M. E.
Grassi de Gende, Ángela Ofelia
author Cuneo, M. E.
author_facet Cuneo, M. E.
Grassi de Gende, Ángela Ofelia
author_role author
author2 Grassi de Gende, Ángela Ofelia
author2_role author
dc.subject.none.fl_str_mv Ciencias Médicas
spontaneously hypertensive rats
cardiac hypertrophy
sarcoplasmic reticulum
Ca 2+ uptake
Ca 2+ ATPase activity
topic Ciencias Médicas
spontaneously hypertensive rats
cardiac hypertrophy
sarcoplasmic reticulum
Ca 2+ uptake
Ca 2+ ATPase activity
dc.description.none.fl_txt_mv Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168±4 and 202±6 mm Hg, respectively). The amount of SR protein (mg/g of left ventricle) was higher when obtained from hypertrophic ventricles of both hypertensive groups than from ventricles of the control group. The result agreed with the enhanced Ca2+ uptake exhibited by left ventricular homogenates of the hypertensive groups. Consequently, Ca2+ uptake in SR microsomes isolated per gram of left ventricle (nmol Ca2+/g muscle) was 51.62±10.06 and 64.99±12.84 in mildly and severely hypertensive groups vs. 17.37±5.79 in the control group (P<0.05). The SR microsomes obtained from ventricles of hypertensive rats showed an enhanced Ca2+ activated ATPase activity that was not accompanied by increased Ca2+ uptake at saturating calcium concentrations, but by increased affinity for calcium (K'app of 1.09±0.28 and 2.67±0.16 μM in SR microsomes of hypertrophic and control ventricles respectively; P<0.05). The rats of calcium loss, measured in SR vesicles passively loaded with45Ca, were similar when assayed in SR obtained from ventricles of both hypertensive and normal rats. These results enable us to suggest that in hearts of rats presenting spontaneous hypertension, the function of the SR system could account for a normal handling of cytosolic calcium. They might support the absence of mechanical alterations described in hearts of young rats of the SHR strain.
Centro de Investigaciones Cardiovasculares
description Sarcoplasmic reticulum (SR) from left ventricles of rats that developed spontaneous hypertension was studied in vitro. Similar increases of left ventricular mass were found when grouping the animals into mild and severe hypertensives (average systolic arterial pressure of 168±4 and 202±6 mm Hg, respectively). The amount of SR protein (mg/g of left ventricle) was higher when obtained from hypertrophic ventricles of both hypertensive groups than from ventricles of the control group. The result agreed with the enhanced Ca2+ uptake exhibited by left ventricular homogenates of the hypertensive groups. Consequently, Ca2+ uptake in SR microsomes isolated per gram of left ventricle (nmol Ca2+/g muscle) was 51.62±10.06 and 64.99±12.84 in mildly and severely hypertensive groups vs. 17.37±5.79 in the control group (P<0.05). The SR microsomes obtained from ventricles of hypertensive rats showed an enhanced Ca2+ activated ATPase activity that was not accompanied by increased Ca2+ uptake at saturating calcium concentrations, but by increased affinity for calcium (K'app of 1.09±0.28 and 2.67±0.16 μM in SR microsomes of hypertrophic and control ventricles respectively; P<0.05). The rats of calcium loss, measured in SR vesicles passively loaded with45Ca, were similar when assayed in SR obtained from ventricles of both hypertensive and normal rats. These results enable us to suggest that in hearts of rats presenting spontaneous hypertension, the function of the SR system could account for a normal handling of cytosolic calcium. They might support the absence of mechanical alterations described in hearts of young rats of the SHR strain.
publishDate 1988
dc.date.none.fl_str_mv 1988
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141080
url http://sedici.unlp.edu.ar/handle/10915/141080
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0300-8428
info:eu-repo/semantics/altIdentifier/issn/1435-1803
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01907362
info:eu-repo/semantics/altIdentifier/pmid/2843160
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
286-295
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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