Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats

Autores
Aiello, Ernesto Alejandro; Grassi de Gende, Ángela Ofelia
Año de publicación
1995
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a lower rate of Ca leak than that in aorta from WKY rats. This slow component was stimulated during washing with 0.03, 0.3, 1 or 10μM free Ca2+. The release of 45Ca induced by Ca2+ proceeded at similar rates in preparations of the two strains. Compared to WKY aortic strips the stimulated efflux of 45Ca was greater in SHR aortic strips due to the higher Ca content. About half of the release of 45Ca induced by 1μM free Ca2+ during the first 6 minutes of stimulation was blocked by 0.6mM of ryanodine or 50μM of ruthenium red, thus identifying the sarcoplasmic reticulum as a source of Ca release. The results suggest that this intracellular storage of Ca in aorta from genetically hypertensive rats is relevant for the generation of high levels of cytosolic Ca2+.
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145232
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/145232
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive ratsAiello, Ernesto AlejandroGrassi de Gende, Ángela OfeliaCiencias MédicasGenetically hypertensive ratsEGTA-skinned aortic stripsCa2+-inducedCa2+ releaseSarcoplasmic reticulumThe release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a lower rate of Ca leak than that in aorta from WKY rats. This slow component was stimulated during washing with 0.03, 0.3, 1 or 10μM free Ca2+. The release of 45Ca induced by Ca2+ proceeded at similar rates in preparations of the two strains. Compared to WKY aortic strips the stimulated efflux of 45Ca was greater in SHR aortic strips due to the higher Ca content. About half of the release of 45Ca induced by 1μM free Ca2+ during the first 6 minutes of stimulation was blocked by 0.6mM of ryanodine or 50μM of ruthenium red, thus identifying the sarcoplasmic reticulum as a source of Ca release. The results suggest that this intracellular storage of Ca in aorta from genetically hypertensive rats is relevant for the generation of high levels of cytosolic Ca2+.Centro de Investigaciones Cardiovasculares1995info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf247-257http://sedici.unlp.edu.ar/handle/10915/145232enginfo:eu-repo/semantics/altIdentifier/issn/0021-4868info:eu-repo/semantics/altIdentifier/issn/1348-673Xinfo:eu-repo/semantics/altIdentifier/doi/10.1536/ihj.36.247info:eu-repo/semantics/altIdentifier/pmid/7596044info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/145232Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.149SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
spellingShingle Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
Aiello, Ernesto Alejandro
Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
title_short Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_full Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_fullStr Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_full_unstemmed Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
title_sort Calcium-induced calcium release in EGTA-skinned aortic strips from genetically hypertensive and normotensive rats
dc.creator.none.fl_str_mv Aiello, Ernesto Alejandro
Grassi de Gende, Ángela Ofelia
author Aiello, Ernesto Alejandro
author_facet Aiello, Ernesto Alejandro
Grassi de Gende, Ángela Ofelia
author_role author
author2 Grassi de Gende, Ángela Ofelia
author2_role author
dc.subject.none.fl_str_mv Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
topic Ciencias Médicas
Genetically hypertensive rats
EGTA-skinned aortic strips
Ca2+-induced
Ca2+ release
Sarcoplasmic reticulum
dc.description.none.fl_txt_mv The release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a lower rate of Ca leak than that in aorta from WKY rats. This slow component was stimulated during washing with 0.03, 0.3, 1 or 10μM free Ca2+. The release of 45Ca induced by Ca2+ proceeded at similar rates in preparations of the two strains. Compared to WKY aortic strips the stimulated efflux of 45Ca was greater in SHR aortic strips due to the higher Ca content. About half of the release of 45Ca induced by 1μM free Ca2+ during the first 6 minutes of stimulation was blocked by 0.6mM of ryanodine or 50μM of ruthenium red, thus identifying the sarcoplasmic reticulum as a source of Ca release. The results suggest that this intracellular storage of Ca in aorta from genetically hypertensive rats is relevant for the generation of high levels of cytosolic Ca2+.
Centro de Investigaciones Cardiovasculares
description The release of 45Ca induced by Ca2+ was studied in genetically hypertensive (SHR) and normotensive (WKY) rats using EGTA-skinned aortic strips. Strips preloaded with 45Ca (pCa 6.6) were desaturated at 5mM of EGTA. A slow component of the washout in aorta from SHRs exhibited higher Ca content and a lower rate of Ca leak than that in aorta from WKY rats. This slow component was stimulated during washing with 0.03, 0.3, 1 or 10μM free Ca2+. The release of 45Ca induced by Ca2+ proceeded at similar rates in preparations of the two strains. Compared to WKY aortic strips the stimulated efflux of 45Ca was greater in SHR aortic strips due to the higher Ca content. About half of the release of 45Ca induced by 1μM free Ca2+ during the first 6 minutes of stimulation was blocked by 0.6mM of ryanodine or 50μM of ruthenium red, thus identifying the sarcoplasmic reticulum as a source of Ca release. The results suggest that this intracellular storage of Ca in aorta from genetically hypertensive rats is relevant for the generation of high levels of cytosolic Ca2+.
publishDate 1995
dc.date.none.fl_str_mv 1995
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145232
url http://sedici.unlp.edu.ar/handle/10915/145232
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0021-4868
info:eu-repo/semantics/altIdentifier/issn/1348-673X
info:eu-repo/semantics/altIdentifier/doi/10.1536/ihj.36.247
info:eu-repo/semantics/altIdentifier/pmid/7596044
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
247-257
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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