Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

Autores
Echeverría, María Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, María Elisa
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
pseudorabies virus
ELISA
enzyme-linked immunosorbent assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83521
Acceder
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network_name_str SEDICI (UNLP)
spelling Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virusEcheverría, María GabrielaNosetto, Edgardo OmarEtcheverrigaray, María ElisaCiencias Veterinariaspseudorabies virusELISAenzyme-linked immunosorbent assayA blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.Facultad de Ciencias Veterinarias2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf266-268http://sedici.unlp.edu.ar/handle/10915/83521enginfo:eu-repo/semantics/altIdentifier/issn/1040-6387info:eu-repo/semantics/altIdentifier/doi/10.1177/104063870001200313info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:09Zoai:sedici.unlp.edu.ar:10915/83521Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:09.894SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
spellingShingle Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
Echeverría, María Gabriela
Ciencias Veterinarias
pseudorabies virus
ELISA
enzyme-linked immunosorbent assay
title_short Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_full Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_fullStr Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_full_unstemmed Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_sort Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
dc.creator.none.fl_str_mv Echeverría, María Gabriela
Nosetto, Edgardo Omar
Etcheverrigaray, María Elisa
author Echeverría, María Gabriela
author_facet Echeverría, María Gabriela
Nosetto, Edgardo Omar
Etcheverrigaray, María Elisa
author_role author
author2 Nosetto, Edgardo Omar
Etcheverrigaray, María Elisa
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
pseudorabies virus
ELISA
enzyme-linked immunosorbent assay
topic Ciencias Veterinarias
pseudorabies virus
ELISA
enzyme-linked immunosorbent assay
dc.description.none.fl_txt_mv A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Facultad de Ciencias Veterinarias
description A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83521
url http://sedici.unlp.edu.ar/handle/10915/83521
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1040-6387
info:eu-repo/semantics/altIdentifier/doi/10.1177/104063870001200313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
266-268
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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