Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)

Autores
Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; Pereyra, N. B.; Pidone, Claudio Luis; Galosi, Cecilia Monica; Etcheverrigaray, Maria Elisa; Nosetto, Edgardo Omar
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infectious virus. The polymerase chain reaction (PCR) technique has provided a sensitive, specific and rapid mean to detect DNA sequences. This study describes a PCR method for detection of pseudorabies virus sequences in swine tissues. In order to determine the presence of suid herpesvirus-1 DNA and antigens, 36 tissue samples collected from 19 dead pigs, with signs of pseudorabies infection, were examined by PCR, virus isolation and indirect immunofluorescence, respectively. Fifteen out of 19 pigs were positive at least for one tissue by PCR (15/19) while only three pseudorabies virus strains were isolated (3/19). All the amplified products were identified by digestion with Sa/l and hybridization. The method described herein circumvents tedious viral isolation and DNA purification and would be a valuable tool for rapid diagnosis, since it would take less than 5 h to reach an accurate result even in poorly preserved tissue samples.
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pereyra, N. B.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Materia
Diagnosis
Pseudorabies Virus
Swine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84848
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)Diagnóstico rápido del virus de la pseudorrabia en tejidos mediante el método de reacción en cadena de la polimerasaEcheverria, Maria GabrielaPecoraro, Marcelo RicardoPereyra, N. B.Pidone, Claudio LuisGalosi, Cecilia MonicaEtcheverrigaray, Maria ElisaNosetto, Edgardo OmarDiagnosisPseudorabies VirusSwinehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infectious virus. The polymerase chain reaction (PCR) technique has provided a sensitive, specific and rapid mean to detect DNA sequences. This study describes a PCR method for detection of pseudorabies virus sequences in swine tissues. In order to determine the presence of suid herpesvirus-1 DNA and antigens, 36 tissue samples collected from 19 dead pigs, with signs of pseudorabies infection, were examined by PCR, virus isolation and indirect immunofluorescence, respectively. Fifteen out of 19 pigs were positive at least for one tissue by PCR (15/19) while only three pseudorabies virus strains were isolated (3/19). All the amplified products were identified by digestion with Sa/l and hybridization. The method described herein circumvents tedious viral isolation and DNA purification and would be a valuable tool for rapid diagnosis, since it would take less than 5 h to reach an accurate result even in poorly preserved tissue samples.Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaFil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaFil: Pereyra, N. B.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaFil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaFil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaFil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; ArgentinaAsociación Argentina de Microbiología2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84848Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; Pereyra, N. B.; Pidone, Claudio Luis; Galosi, Cecilia Monica; et al.; Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR); Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 32; 3; 5-2000; 109-1150325-75411851-7617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.elsevier.es/es-revista-revista-argentina-microbiologia-372-numeros-anterioresinfo:eu-repo/semantics/altIdentifier/url/https://www.aam.org.ar/ram.php?r=21info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:29Zoai:ri.conicet.gov.ar:11336/84848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:29.332CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
Diagnóstico rápido del virus de la pseudorrabia en tejidos mediante el método de reacción en cadena de la polimerasa
title Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
spellingShingle Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
Echeverria, Maria Gabriela
Diagnosis
Pseudorabies Virus
Swine
title_short Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_full Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_fullStr Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_full_unstemmed Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
title_sort Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR)
dc.creator.none.fl_str_mv Echeverria, Maria Gabriela
Pecoraro, Marcelo Ricardo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Monica
Etcheverrigaray, Maria Elisa
Nosetto, Edgardo Omar
author Echeverria, Maria Gabriela
author_facet Echeverria, Maria Gabriela
Pecoraro, Marcelo Ricardo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Monica
Etcheverrigaray, Maria Elisa
Nosetto, Edgardo Omar
author_role author
author2 Pecoraro, Marcelo Ricardo
Pereyra, N. B.
Pidone, Claudio Luis
Galosi, Cecilia Monica
Etcheverrigaray, Maria Elisa
Nosetto, Edgardo Omar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Diagnosis
Pseudorabies Virus
Swine
topic Diagnosis
Pseudorabies Virus
Swine
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infectious virus. The polymerase chain reaction (PCR) technique has provided a sensitive, specific and rapid mean to detect DNA sequences. This study describes a PCR method for detection of pseudorabies virus sequences in swine tissues. In order to determine the presence of suid herpesvirus-1 DNA and antigens, 36 tissue samples collected from 19 dead pigs, with signs of pseudorabies infection, were examined by PCR, virus isolation and indirect immunofluorescence, respectively. Fifteen out of 19 pigs were positive at least for one tissue by PCR (15/19) while only three pseudorabies virus strains were isolated (3/19). All the amplified products were identified by digestion with Sa/l and hybridization. The method described herein circumvents tedious viral isolation and DNA purification and would be a valuable tool for rapid diagnosis, since it would take less than 5 h to reach an accurate result even in poorly preserved tissue samples.
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pereyra, N. B.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Area de Virología; Argentina
description In Argentina pseudorabies is an endemic disease. Routine diagnosis is made by virus isolation. It is a very long procedure to carry out and gives variable results depending on the quality of sample, hence the need for effective techniques, which are rapid and not dependent on the isolation of infectious virus. The polymerase chain reaction (PCR) technique has provided a sensitive, specific and rapid mean to detect DNA sequences. This study describes a PCR method for detection of pseudorabies virus sequences in swine tissues. In order to determine the presence of suid herpesvirus-1 DNA and antigens, 36 tissue samples collected from 19 dead pigs, with signs of pseudorabies infection, were examined by PCR, virus isolation and indirect immunofluorescence, respectively. Fifteen out of 19 pigs were positive at least for one tissue by PCR (15/19) while only three pseudorabies virus strains were isolated (3/19). All the amplified products were identified by digestion with Sa/l and hybridization. The method described herein circumvents tedious viral isolation and DNA purification and would be a valuable tool for rapid diagnosis, since it would take less than 5 h to reach an accurate result even in poorly preserved tissue samples.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84848
Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; Pereyra, N. B.; Pidone, Claudio Luis; Galosi, Cecilia Monica; et al.; Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR); Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 32; 3; 5-2000; 109-115
0325-7541
1851-7617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84848
identifier_str_mv Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; Pereyra, N. B.; Pidone, Claudio Luis; Galosi, Cecilia Monica; et al.; Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction (PCR); Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 32; 3; 5-2000; 109-115
0325-7541
1851-7617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.elsevier.es/es-revista-revista-argentina-microbiologia-372-numeros-anteriores
info:eu-repo/semantics/altIdentifier/url/https://www.aam.org.ar/ram.php?r=21
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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