A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
- Autores
- Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; Agote, Felicitas; Vizoso Pinto, María Guadalupe
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania
Fil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; Argentina
Fil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; Argentina
Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina - Materia
-
HEPATITIS E VIRUS
ELISA
SEROPREVALENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
BLOOD DONORS
RECOMBINANT PROTEIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/121426
Ver los metadatos del registro completo
id |
CONICETDig_51dea5794da3b3ba02416b2527b7f62a |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/121426 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern ArgentinaArce, Lorena PaolaMüller, Melisa FlorenciaMartinez, AlfredoBaiker, ArminMarranzino, GabrielaAgote, FelicitasVizoso Pinto, María GuadalupeHEPATITIS E VIRUSELISASEROPREVALENCEENZYME-LINKED IMMUNOSORBENT ASSAYBLOOD DONORSRECOMBINANT PROTEINhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Baiker, Armin. Bavarian Health and Food Safety Authority; AlemaniaFil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; ArgentinaFil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; ArgentinaFil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFrontiers Research Foundation2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121426Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-24811664-302X1664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02481info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2019.02481/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:21Zoai:ri.conicet.gov.ar:11336/121426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:22.078CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
title |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
spellingShingle |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina Arce, Lorena Paola HEPATITIS E VIRUS ELISA SEROPREVALENCE ENZYME-LINKED IMMUNOSORBENT ASSAY BLOOD DONORS RECOMBINANT PROTEIN |
title_short |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
title_full |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
title_fullStr |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
title_full_unstemmed |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
title_sort |
A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina |
dc.creator.none.fl_str_mv |
Arce, Lorena Paola Müller, Melisa Florencia Martinez, Alfredo Baiker, Armin Marranzino, Gabriela Agote, Felicitas Vizoso Pinto, María Guadalupe |
author |
Arce, Lorena Paola |
author_facet |
Arce, Lorena Paola Müller, Melisa Florencia Martinez, Alfredo Baiker, Armin Marranzino, Gabriela Agote, Felicitas Vizoso Pinto, María Guadalupe |
author_role |
author |
author2 |
Müller, Melisa Florencia Martinez, Alfredo Baiker, Armin Marranzino, Gabriela Agote, Felicitas Vizoso Pinto, María Guadalupe |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
HEPATITIS E VIRUS ELISA SEROPREVALENCE ENZYME-LINKED IMMUNOSORBENT ASSAY BLOOD DONORS RECOMBINANT PROTEIN |
topic |
HEPATITIS E VIRUS ELISA SEROPREVALENCE ENZYME-LINKED IMMUNOSORBENT ASSAY BLOOD DONORS RECOMBINANT PROTEIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance. Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina Fil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina Fil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania Fil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; Argentina Fil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; Argentina Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina |
description |
Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/121426 Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-2481 1664-302X 1664-302X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/121426 |
identifier_str_mv |
Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-2481 1664-302X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02481 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2019.02481/full |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Research Foundation |
publisher.none.fl_str_mv |
Frontiers Research Foundation |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614240286015488 |
score |
13.070432 |