A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina

Autores
Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; Agote, Felicitas; Vizoso Pinto, María Guadalupe
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania
Fil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; Argentina
Fil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; Argentina
Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Materia
HEPATITIS E VIRUS
ELISA
SEROPREVALENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
BLOOD DONORS
RECOMBINANT PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121426
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/121426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern ArgentinaArce, Lorena PaolaMüller, Melisa FlorenciaMartinez, AlfredoBaiker, ArminMarranzino, GabrielaAgote, FelicitasVizoso Pinto, María GuadalupeHEPATITIS E VIRUSELISASEROPREVALENCEENZYME-LINKED IMMUNOSORBENT ASSAYBLOOD DONORSRECOMBINANT PROTEINhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Baiker, Armin. Bavarian Health and Food Safety Authority; AlemaniaFil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; ArgentinaFil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; ArgentinaFil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFrontiers Research Foundation2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121426Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-24811664-302X1664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02481info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2019.02481/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:21Zoai:ri.conicet.gov.ar:11336/121426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:22.078CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
title A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
spellingShingle A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
Arce, Lorena Paola
HEPATITIS E VIRUS
ELISA
SEROPREVALENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
BLOOD DONORS
RECOMBINANT PROTEIN
title_short A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
title_full A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
title_fullStr A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
title_full_unstemmed A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
title_sort A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina
dc.creator.none.fl_str_mv Arce, Lorena Paola
Müller, Melisa Florencia
Martinez, Alfredo
Baiker, Armin
Marranzino, Gabriela
Agote, Felicitas
Vizoso Pinto, María Guadalupe
author Arce, Lorena Paola
author_facet Arce, Lorena Paola
Müller, Melisa Florencia
Martinez, Alfredo
Baiker, Armin
Marranzino, Gabriela
Agote, Felicitas
Vizoso Pinto, María Guadalupe
author_role author
author2 Müller, Melisa Florencia
Martinez, Alfredo
Baiker, Armin
Marranzino, Gabriela
Agote, Felicitas
Vizoso Pinto, María Guadalupe
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HEPATITIS E VIRUS
ELISA
SEROPREVALENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
BLOOD DONORS
RECOMBINANT PROTEIN
topic HEPATITIS E VIRUS
ELISA
SEROPREVALENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
BLOOD DONORS
RECOMBINANT PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Müller, Melisa Florencia. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Martinez, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Baiker, Armin. Bavarian Health and Food Safety Authority; Alemania
Fil: Marranzino, Gabriela. Gobierno de la provincia de Tucumán. Ministerio de Salud. Banco Central de Sangre de Tucumán “Dr. César Guerra”; Argentina
Fil: Agote, Felicitas. Gobierno de la provincia de Tucumán. Sistema Provincial de Salud. Banco Central de Sangre "Dr. César Guerra" ; Argentina
Fil: Vizoso Pinto, María Guadalupe. Universidad Nacional de Tucumán. Instituto de Investigaciones En Medicina Molecular y Celular Aplicada del Bicentenario. - Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
description Hepatitis E virus (HEV) is an emergent virus that causes acute hepatitis in immunocompetent hosts and chronic hepatitis in immunocompromised ones. In Latin America, the main circulating genotype (GT) is 3, which is usually of zoonotic origin. Diagnosis and seroprevalence studies mainly rely on the detection of specific antibodies. In Argentina there are scarce data on the seroprevalence of HEV infection mainly due to the lack of awareness of HEV circulation and because HEV testing is not included in routine assays. The following conditions were determined: an optimal antigen concentration of 0.25 µg/ml, a serum dilution of 1:80, gelatin as blocking agent, and a secondary antibody dilution of 1:1000. Specificity (97.8%) and sensibility (100%) were determined using a panel of previously characterized sera. When we compared our in-house ELISA with a commercial test (DIA.PRO), we obtained a kappa index of 0.939. We screened 813 blood donor samples with this newly developed ELISA and found a seroprevalence of 8.31%. We further analyzed the seroprevalence of blood transmitted diseases such as Chagas disease (1.9%), syphilis (1.2%), hepatitis B (0.9%), and brucellosis (0.4%) in a group of 412 blood donors. These samples did not cross reacted with HEV positive samples according to the χ2 test for comparison of proportions. We show for the first time, the circulation of HEV in Tucuman, the most populated city in northern Argentina. We expect that this study will raise the interest of health decision makers who should intercede to include indirect testing of HEV in regular diagnostic protocols. In conclusion, the in-house ELISA developed in this work shows a very good agreement with an already licensed commercial HEV IgG ELISA (DIA.PRO, ITALY), which can be used as an epidemiologic tool for HEV surveillance.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121426
Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-2481
1664-302X
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121426
identifier_str_mv Arce, Lorena Paola; Müller, Melisa Florencia; Martinez, Alfredo; Baiker, Armin; Marranzino, Gabriela; et al.; A Novel In-House Enzyme-Linked Immunosorbent Assay for Genotype 3 Hepatitis E Virus Reveals High Seroprevalence in Blood Donors in Northern Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 10; 11-2019; 1-9;2481-2481
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02481
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2019.02481/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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