Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

Autores
Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S.S.; Nascimento, Leile Camila J.; Portilho, Moyra M.; Soares, Gúbio C.; Weaver, Scott C.; Reis, Mitermayer G.; Kitron, Uriel D.; Ribeiro, Guilherme S.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.
Fil: Kikuti, Mariana. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Moreira, Patrícia S.S.. Fundación Oswaldo Cruz; Brasil
Fil: Nascimento, Leile Camila J.. Fundación Oswaldo Cruz; Brasil
Fil: Portilho, Moyra M.. Fundación Oswaldo Cruz; Brasil
Fil: Soares, Gúbio C.. Universidade Federal da Bahia; Brasil
Fil: Weaver, Scott C.. University of Texas Medical Branch; Estados Unidos
Fil: Reis, Mitermayer G.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados Unidos
Fil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Materia
ACCURACY
CHIKUNGUNYA VIRUS
DIAGNOSTIC PERFORMANCE
ELISA
ENZYME-LINKED IMMUNOSORBENT ASSAY
SENSITIVITY AND SPECIFICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111497
Acceder
id CONICETDig_bb2a4935f4a40002ba7662fabacc3791
oai_identifier_str oai:ri.conicet.gov.ar:11336/111497
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika virusesKikuti, MarianaTauro, Laura BeatrizMoreira, Patrícia S.S.Nascimento, Leile Camila J.Portilho, Moyra M.Soares, Gúbio C.Weaver, Scott C.Reis, Mitermayer G.Kitron, Uriel D.Ribeiro, Guilherme S.ACCURACYCHIKUNGUNYA VIRUSDIAGNOSTIC PERFORMANCEELISAENZYME-LINKED IMMUNOSORBENT ASSAYSENSITIVITY AND SPECIFICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.Fil: Kikuti, Mariana. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; BrasilFil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Moreira, Patrícia S.S.. Fundación Oswaldo Cruz; BrasilFil: Nascimento, Leile Camila J.. Fundación Oswaldo Cruz; BrasilFil: Portilho, Moyra M.. Fundación Oswaldo Cruz; BrasilFil: Soares, Gúbio C.. Universidade Federal da Bahia; BrasilFil: Weaver, Scott C.. University of Texas Medical Branch; Estados UnidosFil: Reis, Mitermayer G.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; BrasilFil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados UnidosFil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; BrasilElsevier2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111497Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S.S.; Nascimento, Leile Camila J.; Portilho, Moyra M.; et al.; Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses; Elsevier; International Journal of Infectious Diseases; 91; 2-2020; 38-431201-9712CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijid.2019.11.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1201971219304400info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:06Zoai:ri.conicet.gov.ar:11336/111497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:06.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
title Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
spellingShingle Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
Kikuti, Mariana
ACCURACY
CHIKUNGUNYA VIRUS
DIAGNOSTIC PERFORMANCE
ELISA
ENZYME-LINKED IMMUNOSORBENT ASSAY
SENSITIVITY AND SPECIFICITY
title_short Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
title_full Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
title_fullStr Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
title_full_unstemmed Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
title_sort Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
dc.creator.none.fl_str_mv Kikuti, Mariana
Tauro, Laura Beatriz
Moreira, Patrícia S.S.
Nascimento, Leile Camila J.
Portilho, Moyra M.
Soares, Gúbio C.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author Kikuti, Mariana
author_facet Kikuti, Mariana
Tauro, Laura Beatriz
Moreira, Patrícia S.S.
Nascimento, Leile Camila J.
Portilho, Moyra M.
Soares, Gúbio C.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author_role author
author2 Tauro, Laura Beatriz
Moreira, Patrícia S.S.
Nascimento, Leile Camila J.
Portilho, Moyra M.
Soares, Gúbio C.
Weaver, Scott C.
Reis, Mitermayer G.
Kitron, Uriel D.
Ribeiro, Guilherme S.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACCURACY
CHIKUNGUNYA VIRUS
DIAGNOSTIC PERFORMANCE
ELISA
ENZYME-LINKED IMMUNOSORBENT ASSAY
SENSITIVITY AND SPECIFICITY
topic ACCURACY
CHIKUNGUNYA VIRUS
DIAGNOSTIC PERFORMANCE
ELISA
ENZYME-LINKED IMMUNOSORBENT ASSAY
SENSITIVITY AND SPECIFICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.
Fil: Kikuti, Mariana. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Tauro, Laura Beatriz. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Moreira, Patrícia S.S.. Fundación Oswaldo Cruz; Brasil
Fil: Nascimento, Leile Camila J.. Fundación Oswaldo Cruz; Brasil
Fil: Portilho, Moyra M.. Fundación Oswaldo Cruz; Brasil
Fil: Soares, Gúbio C.. Universidade Federal da Bahia; Brasil
Fil: Weaver, Scott C.. University of Texas Medical Branch; Estados Unidos
Fil: Reis, Mitermayer G.. Fundación Oswaldo Cruz; Brasil. Universidade Federal da Bahia; Brasil
Fil: Kitron, Uriel D.. Fundación Oswaldo Cruz; Brasil. University of Emory; Estados Unidos
Fil: Ribeiro, Guilherme S.. Universidade Federal da Bahia; Brasil. Fundación Oswaldo Cruz; Brasil
description Objective To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111497
Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S.S.; Nascimento, Leile Camila J.; Portilho, Moyra M.; et al.; Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses; Elsevier; International Journal of Infectious Diseases; 91; 2-2020; 38-43
1201-9712
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111497
identifier_str_mv Kikuti, Mariana; Tauro, Laura Beatriz; Moreira, Patrícia S.S.; Nascimento, Leile Camila J.; Portilho, Moyra M.; et al.; Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses; Elsevier; International Journal of Infectious Diseases; 91; 2-2020; 38-43
1201-9712
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijid.2019.11.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1201971219304400
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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