Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

Autores
Echeverria, Maria Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, Maria Elisa
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Elisa
Urease
Aujeszky
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84164
Acceder
id CONICETDig_b8dbd9be9865ce50cc0c4fd756e6a3d5
oai_identifier_str oai:ri.conicet.gov.ar:11336/84164
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virusEcheverria, Maria GabrielaNosetto, Edgardo OmarEtcheverrigaray, Maria ElisaElisaUreaseAujeszkyhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaAmerican Association of Veterinary Laboratory Diagnosticians2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84164Echeverria, Maria Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, Maria Elisa; Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 12; 3; 5-2000; 266-2681040-6387CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/104063870001200313info:eu-repo/semantics/altIdentifier/url/journals.sagepub.com/doi/10.1177/104063870001200313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:21Zoai:ri.conicet.gov.ar:11336/84164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:21.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
spellingShingle Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
Echeverria, Maria Gabriela
Elisa
Urease
Aujeszky
title_short Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_full Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_fullStr Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_full_unstemmed Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
title_sort Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
dc.creator.none.fl_str_mv Echeverria, Maria Gabriela
Nosetto, Edgardo Omar
Etcheverrigaray, Maria Elisa
author Echeverria, Maria Gabriela
author_facet Echeverria, Maria Gabriela
Nosetto, Edgardo Omar
Etcheverrigaray, Maria Elisa
author_role author
author2 Nosetto, Edgardo Omar
Etcheverrigaray, Maria Elisa
author2_role author
author
dc.subject.none.fl_str_mv Elisa
Urease
Aujeszky
topic Elisa
Urease
Aujeszky
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Etcheverrigaray, Maria Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84164
Echeverria, Maria Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, Maria Elisa; Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 12; 3; 5-2000; 266-268
1040-6387
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84164
identifier_str_mv Echeverria, Maria Gabriela; Nosetto, Edgardo Omar; Etcheverrigaray, Maria Elisa; Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 12; 3; 5-2000; 266-268
1040-6387
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/104063870001200313
info:eu-repo/semantics/altIdentifier/url/journals.sagepub.com/doi/10.1177/104063870001200313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614517304066048
score 13.069144

Publicaciones similares