Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration

Autores
Rodrigo Albors, Aida; Tazaki, Akira; Rost, Fabian; Nowoshilow, Sergej; Chara, Osvaldo; Tanaka, Ely M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
Facultad de Ciencias Exactas
Instituto de Física de Líquidos y Sistemas Biológicos
Materia
Ciencias Exactas
Regeneration
Spinal cord
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86399
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/86399
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regenerationRodrigo Albors, AidaTazaki, AkiraRost, FabianNowoshilow, SergejChara, OsvaldoTanaka, Ely M.Ciencias ExactasRegenerationSpinal cordAxolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.Facultad de Ciencias ExactasInstituto de Física de Líquidos y Sistemas Biológicos2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86399enginfo:eu-repo/semantics/altIdentifier/issn/2050-084Xinfo:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.10230info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:08Zoai:sedici.unlp.edu.ar:10915/86399Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:08.968SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
spellingShingle Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
Rodrigo Albors, Aida
Ciencias Exactas
Regeneration
Spinal cord
title_short Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_fullStr Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full_unstemmed Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_sort Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
dc.creator.none.fl_str_mv Rodrigo Albors, Aida
Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
author Rodrigo Albors, Aida
author_facet Rodrigo Albors, Aida
Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
author_role author
author2 Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Regeneration
Spinal cord
topic Ciencias Exactas
Regeneration
Spinal cord
dc.description.none.fl_txt_mv Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
Facultad de Ciencias Exactas
Instituto de Física de Líquidos y Sistemas Biológicos
description Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86399
url http://sedici.unlp.edu.ar/handle/10915/86399
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2050-084X
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.10230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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