Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration

Autores
Rodrigo Albors, Aida; Tazaky, Akira; Rost, Fabian; Nowoshilow, Sergej; Chara, Osvaldo; Tanaka, Elly M
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
Fil: Rodrigo Albors, Aida. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Tazaky, Akira. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Rost, Fabian. Technische Universitat Dresden; Alemania
Fil: Nowoshilow, Sergej. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universitat Dresden; Alemania
Fil: Tanaka, Elly M. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Materia
PLANAR CELL POLARITY
REGENERATION
SPINAL CORD
AXOLOTL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48063
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regenerationRodrigo Albors, AidaTazaky, AkiraRost, FabianNowoshilow, SergejChara, OsvaldoTanaka, Elly MPLANAR CELL POLARITYREGENERATIONSPINAL CORDAXOLOTLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.Fil: Rodrigo Albors, Aida. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; AlemaniaFil: Tazaky, Akira. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; AlemaniaFil: Rost, Fabian. Technische Universitat Dresden; AlemaniaFil: Nowoshilow, Sergej. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; AlemaniaFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universitat Dresden; AlemaniaFil: Tanaka, Elly M. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; AlemaniaeLife Sciences Publications2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48063Rodrigo Albors, Aida; Tazaky, Akira; Rost, Fabian; Nowoshilow, Sergej; Chara, Osvaldo; et al.; Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration; eLife Sciences Publications; eLife; 2015; 11-2015; 1-292050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.10230info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/10230info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:47Zoai:ri.conicet.gov.ar:11336/48063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:47.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
spellingShingle Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
Rodrigo Albors, Aida
PLANAR CELL POLARITY
REGENERATION
SPINAL CORD
AXOLOTL
title_short Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_fullStr Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full_unstemmed Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_sort Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
dc.creator.none.fl_str_mv Rodrigo Albors, Aida
Tazaky, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Elly M
author Rodrigo Albors, Aida
author_facet Rodrigo Albors, Aida
Tazaky, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Elly M
author_role author
author2 Tazaky, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Elly M
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PLANAR CELL POLARITY
REGENERATION
SPINAL CORD
AXOLOTL
topic PLANAR CELL POLARITY
REGENERATION
SPINAL CORD
AXOLOTL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
Fil: Rodrigo Albors, Aida. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Tazaky, Akira. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Rost, Fabian. Technische Universitat Dresden; Alemania
Fil: Nowoshilow, Sergej. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universitat Dresden; Alemania
Fil: Tanaka, Elly M. Deutsche Forschungsgemeinschaft; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania. Technische Universitat Dresden; Alemania
description Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48063
Rodrigo Albors, Aida; Tazaky, Akira; Rost, Fabian; Nowoshilow, Sergej; Chara, Osvaldo; et al.; Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration; eLife Sciences Publications; eLife; 2015; 11-2015; 1-29
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48063
identifier_str_mv Rodrigo Albors, Aida; Tazaky, Akira; Rost, Fabian; Nowoshilow, Sergej; Chara, Osvaldo; et al.; Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration; eLife Sciences Publications; eLife; 2015; 11-2015; 1-29
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.10230
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/10230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614510069940224
score 13.069144

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