OKB, a novel family of brain-gut neuropeptides from insects

Autores
Sterkel, Marcos; Oliveira, Pedro L.; Urlaub, Henning; Hernández Martínez, Salvador; Rivera Pomar, Rolando Víctor; Ons, Sheila
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of Drosophila sp. (Diptera) and Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
Centro Regional de Estudios Genómicos
Materia
Biología
Neuropeptides
Orcokinins
Insects
Rhodnius prolixus
Chagas’ disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127976

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network_name_str SEDICI (UNLP)
spelling OKB, a novel family of brain-gut neuropeptides from insectsSterkel, MarcosOliveira, Pedro L.Urlaub, HenningHernández Martínez, SalvadorRivera Pomar, Rolando VíctorOns, SheilaBiologíaNeuropeptidesOrcokininsInsectsRhodnius prolixusChagas’ diseaseIn insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of Drosophila sp. (Diptera) and Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.Centro Regional de Estudios Genómicos2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf466-473http://sedici.unlp.edu.ar/handle/10915/127976enginfo:eu-repo/semantics/altIdentifier/issn/1879-0240info:eu-repo/semantics/altIdentifier/issn/0965-1748info:eu-repo/semantics/altIdentifier/pmid/22480496info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2012.03.003info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:02Zoai:sedici.unlp.edu.ar:10915/127976Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:02.844SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv OKB, a novel family of brain-gut neuropeptides from insects
title OKB, a novel family of brain-gut neuropeptides from insects
spellingShingle OKB, a novel family of brain-gut neuropeptides from insects
Sterkel, Marcos
Biología
Neuropeptides
Orcokinins
Insects
Rhodnius prolixus
Chagas’ disease
title_short OKB, a novel family of brain-gut neuropeptides from insects
title_full OKB, a novel family of brain-gut neuropeptides from insects
title_fullStr OKB, a novel family of brain-gut neuropeptides from insects
title_full_unstemmed OKB, a novel family of brain-gut neuropeptides from insects
title_sort OKB, a novel family of brain-gut neuropeptides from insects
dc.creator.none.fl_str_mv Sterkel, Marcos
Oliveira, Pedro L.
Urlaub, Henning
Hernández Martínez, Salvador
Rivera Pomar, Rolando Víctor
Ons, Sheila
author Sterkel, Marcos
author_facet Sterkel, Marcos
Oliveira, Pedro L.
Urlaub, Henning
Hernández Martínez, Salvador
Rivera Pomar, Rolando Víctor
Ons, Sheila
author_role author
author2 Oliveira, Pedro L.
Urlaub, Henning
Hernández Martínez, Salvador
Rivera Pomar, Rolando Víctor
Ons, Sheila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Neuropeptides
Orcokinins
Insects
Rhodnius prolixus
Chagas’ disease
topic Biología
Neuropeptides
Orcokinins
Insects
Rhodnius prolixus
Chagas’ disease
dc.description.none.fl_txt_mv In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of Drosophila sp. (Diptera) and Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
Centro Regional de Estudios Genómicos
description In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of Drosophila sp. (Diptera) and Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127976
url http://sedici.unlp.edu.ar/handle/10915/127976
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1879-0240
info:eu-repo/semantics/altIdentifier/issn/0965-1748
info:eu-repo/semantics/altIdentifier/pmid/22480496
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2012.03.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
466-473
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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