OKB, a novel family of brain-gut neuropeptides from insects

Autores
Sterkel, Marcos; Oliveira, Pedro L.; Urlaub, Henning; Hernandez Martinez, Salvador; Rivera Pomar, Rolando; Ons, Sheila
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran . Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of . Drosophila sp. (Diptera) and . Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of . R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
Fil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Urlaub, Henning. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hernandez Martinez, Salvador. Instituo Nacional de Salud Pública; México
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Materia
Chagas' Disease
Insects
Neuropeptides
Orcokinins
Rhodnius Prolixus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75929
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling OKB, a novel family of brain-gut neuropeptides from insectsSterkel, MarcosOliveira, Pedro L.Urlaub, HenningHernandez Martinez, SalvadorRivera Pomar, RolandoOns, SheilaChagas' DiseaseInsectsNeuropeptidesOrcokininsRhodnius Prolixushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran . Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of . Drosophila sp. (Diptera) and . Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of . R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.Fil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; BrasilFil: Urlaub, Henning. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hernandez Martinez, Salvador. Instituo Nacional de Salud Pública; MéxicoFil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaPergamon-Elsevier Science Ltd2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75929Sterkel, Marcos; Oliveira, Pedro L.; Urlaub, Henning; Hernandez Martinez, Salvador; Rivera Pomar, Rolando; et al.; OKB, a novel family of brain-gut neuropeptides from insects; Pergamon-Elsevier Science Ltd; Insect Biochemistry and Molecular Biology; 42; 7; 7-2012; 466-4730965-1748CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2012.03.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174812000422info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:01Zoai:ri.conicet.gov.ar:11336/75929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:01.604CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv OKB, a novel family of brain-gut neuropeptides from insects
title OKB, a novel family of brain-gut neuropeptides from insects
spellingShingle OKB, a novel family of brain-gut neuropeptides from insects
Sterkel, Marcos
Chagas' Disease
Insects
Neuropeptides
Orcokinins
Rhodnius Prolixus
title_short OKB, a novel family of brain-gut neuropeptides from insects
title_full OKB, a novel family of brain-gut neuropeptides from insects
title_fullStr OKB, a novel family of brain-gut neuropeptides from insects
title_full_unstemmed OKB, a novel family of brain-gut neuropeptides from insects
title_sort OKB, a novel family of brain-gut neuropeptides from insects
dc.creator.none.fl_str_mv Sterkel, Marcos
Oliveira, Pedro L.
Urlaub, Henning
Hernandez Martinez, Salvador
Rivera Pomar, Rolando
Ons, Sheila
author Sterkel, Marcos
author_facet Sterkel, Marcos
Oliveira, Pedro L.
Urlaub, Henning
Hernandez Martinez, Salvador
Rivera Pomar, Rolando
Ons, Sheila
author_role author
author2 Oliveira, Pedro L.
Urlaub, Henning
Hernandez Martinez, Salvador
Rivera Pomar, Rolando
Ons, Sheila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chagas' Disease
Insects
Neuropeptides
Orcokinins
Rhodnius Prolixus
topic Chagas' Disease
Insects
Neuropeptides
Orcokinins
Rhodnius Prolixus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran . Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of . Drosophila sp. (Diptera) and . Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of . R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
Fil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Urlaub, Henning. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hernandez Martinez, Salvador. Instituo Nacional de Salud Pública; México
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
description In insects, neuropeptides play a central role in the control of most physiological processes. The knowledge and characterization of new neuropeptide families, is of interest on the fields of Genetics, Genomics, Neurobiology, Endocrinology and Evolution. This knowledge also provides the tools for the design of peptidomimetics, pseudopeptides or small molecules, capable of disrupting the physiological processes regulated by the signaling molecules and their receptors. This is a promising target for a novel generation of insecticides. Using database searches, mass spectrometry and RACE-PCR, we identified a neuropeptide precursor transcript encoding a new family of insect neuropeptides in the hemipteran . Rhodnius prolixus. We named this precursor Orcokinin B, because is originated by the alternative splicing of the Orcokinin gen. EST and genomic data suggests that Orcokinin B is expressed in the nervous system and gut from several insect species, with the exception of . Drosophila sp. (Diptera) and . Acyirthosiphon pisum (Hemiptera). Mass spectrometry and RT-PCR confirmed the expression of Orcokinin B in brain and anterior midgut of . R. prolixus. Furthermore, we identified orthologues of this new family of peptides in genomic and EST databases from Arachnids and Crustaceans.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75929
Sterkel, Marcos; Oliveira, Pedro L.; Urlaub, Henning; Hernandez Martinez, Salvador; Rivera Pomar, Rolando; et al.; OKB, a novel family of brain-gut neuropeptides from insects; Pergamon-Elsevier Science Ltd; Insect Biochemistry and Molecular Biology; 42; 7; 7-2012; 466-473
0965-1748
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75929
identifier_str_mv Sterkel, Marcos; Oliveira, Pedro L.; Urlaub, Henning; Hernandez Martinez, Salvador; Rivera Pomar, Rolando; et al.; OKB, a novel family of brain-gut neuropeptides from insects; Pergamon-Elsevier Science Ltd; Insect Biochemistry and Molecular Biology; 42; 7; 7-2012; 466-473
0965-1748
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2012.03.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174812000422
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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