Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus

Autores
Ons, Sheila; Sterkel, Marcos; Diambra, Luis Anibal; Urlaub, H.; Rivera Pomar, Rolando
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Sterkel, Marcos. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Urlaub, H.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
GENOMIC
INSECT NEUROPEPTIDES
PEPTIDOMIC
RHODNIUS PROLIXUS
TRANSCRIPTOMIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193692
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixusOns, SheilaSterkel, MarcosDiambra, Luis AnibalUrlaub, H.Rivera Pomar, RolandoGENOMICINSECT NEUROPEPTIDESPEPTIDOMICRHODNIUS PROLIXUSTRANSCRIPTOMIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Sterkel, Marcos. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Urlaub, H.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Rivera Pomar, Rolando. Universidad Nacional de La Plata; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaWiley Blackwell Publishing, Inc2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193692Ons, Sheila; Sterkel, Marcos; Diambra, Luis Anibal; Urlaub, H.; Rivera Pomar, Rolando; Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus; Wiley Blackwell Publishing, Inc; Insect Molecular Biology; 20; 1; 1-2011; 29-440962-1075CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2583.2010.01050.xinfo:eu-repo/semantics/altIdentifier/url/https://resjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2583.2010.01050.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:30Zoai:ri.conicet.gov.ar:11336/193692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:30.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
title Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
spellingShingle Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
Ons, Sheila
GENOMIC
INSECT NEUROPEPTIDES
PEPTIDOMIC
RHODNIUS PROLIXUS
TRANSCRIPTOMIC
title_short Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
title_full Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
title_fullStr Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
title_full_unstemmed Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
title_sort Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus
dc.creator.none.fl_str_mv Ons, Sheila
Sterkel, Marcos
Diambra, Luis Anibal
Urlaub, H.
Rivera Pomar, Rolando
author Ons, Sheila
author_facet Ons, Sheila
Sterkel, Marcos
Diambra, Luis Anibal
Urlaub, H.
Rivera Pomar, Rolando
author_role author
author2 Sterkel, Marcos
Diambra, Luis Anibal
Urlaub, H.
Rivera Pomar, Rolando
author2_role author
author
author
author
dc.subject.none.fl_str_mv GENOMIC
INSECT NEUROPEPTIDES
PEPTIDOMIC
RHODNIUS PROLIXUS
TRANSCRIPTOMIC
topic GENOMIC
INSECT NEUROPEPTIDES
PEPTIDOMIC
RHODNIUS PROLIXUS
TRANSCRIPTOMIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Sterkel, Marcos. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Diambra, Luis Anibal. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Urlaub, H.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193692
Ons, Sheila; Sterkel, Marcos; Diambra, Luis Anibal; Urlaub, H.; Rivera Pomar, Rolando; Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus; Wiley Blackwell Publishing, Inc; Insect Molecular Biology; 20; 1; 1-2011; 29-44
0962-1075
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193692
identifier_str_mv Ons, Sheila; Sterkel, Marcos; Diambra, Luis Anibal; Urlaub, H.; Rivera Pomar, Rolando; Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus; Wiley Blackwell Publishing, Inc; Insect Molecular Biology; 20; 1; 1-2011; 29-44
0962-1075
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2583.2010.01050.x
info:eu-repo/semantics/altIdentifier/url/https://resjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2583.2010.01050.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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