Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves

Autores
Büchert, Agustín M.; Civello, Pedro Marcos; Martínez, Gustavo Adolfo
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The removal of Mg2+ is an important step in the chlorophyll degradation pathway and extracts from senescent and presenescent Arabidopsis thaliana leaves were analyzed for Mg-dechelatase activity, using chlorophyllin, an artificial derivative of the natural substrate, chlorophyllide. The optimum temperature and pH for this reaction were determined to be at approximately 50 °C and 7.2, respectively. Mg-dechelatase activity was enhanced by addition of EDTA and inhibited by MgCl2, HgCl2 and reduced glutathione, indicating phenomenons such as retroinhibition by reaction products and dependence on the redox state of the mixture. Size exclusion chromatography was performed on Arabidopsis leaf extracts, and Mg-dechelatase activity was found in the fraction corresponding to molecular mass of about 42 kDa, which indicates that the Mg-dechelating compound in Arabidopsis is considerably larger than in other systems. During dark-induced senescence, the activity increased over time until reaching a maximum at day 4, and then decreased. The addition of plant growth regulators indicated that the accumulation of Mg-dechelatase was activated by ethylene and delayed by 6-benzylaminopurine.
Instituto de Fisiología Vegetal
Materia
Biología
chlorophyll degradation
chlorophyllin
pheophorbide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/133524
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/133524
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leavesBüchert, Agustín M.Civello, Pedro MarcosMartínez, Gustavo AdolfoBiologíachlorophyll degradationchlorophyllinpheophorbideThe removal of Mg<sup>2+</sup> is an important step in the chlorophyll degradation pathway and extracts from senescent and presenescent <i>Arabidopsis thaliana</i> leaves were analyzed for Mg-dechelatase activity, using chlorophyllin, an artificial derivative of the natural substrate, chlorophyllide. The optimum temperature and pH for this reaction were determined to be at approximately 50 °C and 7.2, respectively. Mg-dechelatase activity was enhanced by addition of EDTA and inhibited by MgCl<sub>2</sub>, HgCl<sub>2</sub> and reduced glutathione, indicating phenomenons such as retroinhibition by reaction products and dependence on the redox state of the mixture. Size exclusion chromatography was performed on <i>Arabidopsis</i> leaf extracts, and Mg-dechelatase activity was found in the fraction corresponding to molecular mass of about 42 kDa, which indicates that the Mg-dechelating compound in <i>Arabidopsis</i> is considerably larger than in other systems. During dark-induced senescence, the activity increased over time until reaching a maximum at day 4, and then decreased. The addition of plant growth regulators indicated that the accumulation of Mg-dechelatase was activated by ethylene and delayed by 6-benzylaminopurine.Instituto de Fisiología Vegetal2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf75-82http://sedici.unlp.edu.ar/handle/10915/133524spainfo:eu-repo/semantics/altIdentifier/issn/0006-3134info:eu-repo/semantics/altIdentifier/issn/1573-8264info:eu-repo/semantics/altIdentifier/doi/10.1007/s10535-011-0010-1info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:47Zoai:sedici.unlp.edu.ar:10915/133524Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:47.259SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
title Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
spellingShingle Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
Büchert, Agustín M.
Biología
chlorophyll degradation
chlorophyllin
pheophorbide
title_short Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
title_full Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
title_fullStr Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
title_full_unstemmed Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
title_sort Characterization of Mg-dechelating substance in senescent and pre-senescent Arabidopsis thaliana leaves
dc.creator.none.fl_str_mv Büchert, Agustín M.
Civello, Pedro Marcos
Martínez, Gustavo Adolfo
author Büchert, Agustín M.
author_facet Büchert, Agustín M.
Civello, Pedro Marcos
Martínez, Gustavo Adolfo
author_role author
author2 Civello, Pedro Marcos
Martínez, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv Biología
chlorophyll degradation
chlorophyllin
pheophorbide
topic Biología
chlorophyll degradation
chlorophyllin
pheophorbide
dc.description.none.fl_txt_mv The removal of Mg<sup>2+</sup> is an important step in the chlorophyll degradation pathway and extracts from senescent and presenescent <i>Arabidopsis thaliana</i> leaves were analyzed for Mg-dechelatase activity, using chlorophyllin, an artificial derivative of the natural substrate, chlorophyllide. The optimum temperature and pH for this reaction were determined to be at approximately 50 °C and 7.2, respectively. Mg-dechelatase activity was enhanced by addition of EDTA and inhibited by MgCl<sub>2</sub>, HgCl<sub>2</sub> and reduced glutathione, indicating phenomenons such as retroinhibition by reaction products and dependence on the redox state of the mixture. Size exclusion chromatography was performed on <i>Arabidopsis</i> leaf extracts, and Mg-dechelatase activity was found in the fraction corresponding to molecular mass of about 42 kDa, which indicates that the Mg-dechelating compound in <i>Arabidopsis</i> is considerably larger than in other systems. During dark-induced senescence, the activity increased over time until reaching a maximum at day 4, and then decreased. The addition of plant growth regulators indicated that the accumulation of Mg-dechelatase was activated by ethylene and delayed by 6-benzylaminopurine.
Instituto de Fisiología Vegetal
description The removal of Mg<sup>2+</sup> is an important step in the chlorophyll degradation pathway and extracts from senescent and presenescent <i>Arabidopsis thaliana</i> leaves were analyzed for Mg-dechelatase activity, using chlorophyllin, an artificial derivative of the natural substrate, chlorophyllide. The optimum temperature and pH for this reaction were determined to be at approximately 50 °C and 7.2, respectively. Mg-dechelatase activity was enhanced by addition of EDTA and inhibited by MgCl<sub>2</sub>, HgCl<sub>2</sub> and reduced glutathione, indicating phenomenons such as retroinhibition by reaction products and dependence on the redox state of the mixture. Size exclusion chromatography was performed on <i>Arabidopsis</i> leaf extracts, and Mg-dechelatase activity was found in the fraction corresponding to molecular mass of about 42 kDa, which indicates that the Mg-dechelating compound in <i>Arabidopsis</i> is considerably larger than in other systems. During dark-induced senescence, the activity increased over time until reaching a maximum at day 4, and then decreased. The addition of plant growth regulators indicated that the accumulation of Mg-dechelatase was activated by ethylene and delayed by 6-benzylaminopurine.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/133524
url http://sedici.unlp.edu.ar/handle/10915/133524
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0006-3134
info:eu-repo/semantics/altIdentifier/issn/1573-8264
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10535-011-0010-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
75-82
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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