Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.

Autores
Gómez Lobato, María Eugenia; Civello, Pedro Marcos; Martinez, Gustavo Adolfo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Broccoli is a highly perishable vegetable that shows enhanced postharvest senescence and intense de‐greening caused by chlorophyll degradation. One of the key steps of chlorophyll catabolism is the opening of chlorophyll tretrapyrrole catalysed by pheophorbide a oxygenase (PaO). In this study the expression of a gene encoding a putative PaO was characterised under several chemical and physical treatments. RESULTS: A fragment of a gene encoding a PaO from broccoli (BoPaO) was cloned. The expression of BoPaO showed an important increment during postharvest senescence, in correlation with chlorophyll degradation. Furthermore, broccoli heads were treated with the hormones cytokinin and ethylene. Cytokinin delayed the increment in BoPaO expression, while ethylene accelerated the process. Also, several postharvest treatments were applied in order to evaluate their effect on BoPaO expression. Samples treated with modified atmosphere, hot air, UV‐C or white light showed a delay in chlorophyll degradation and de‐greening. In most cases the treatments also delayed the increment in BoPaO expression during senescence. CONCLUSION: A close correlation between chlorophyll degradation and BoPaO expression was found during broccoli senescence. This relationship was corroborated in samples treated with different hormonal and physical applications. Copyright © 2011 Society of Chemical Industry
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
Broccoli
Senescence
Chlorophyll Degradation
Pheophorbide a Oxygenase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75246
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/75246
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.Gómez Lobato, María EugeniaCivello, Pedro MarcosMartinez, Gustavo AdolfoBroccoliSenescenceChlorophyll DegradationPheophorbide a Oxygenasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND: Broccoli is a highly perishable vegetable that shows enhanced postharvest senescence and intense de‐greening caused by chlorophyll degradation. One of the key steps of chlorophyll catabolism is the opening of chlorophyll tretrapyrrole catalysed by pheophorbide a oxygenase (PaO). In this study the expression of a gene encoding a putative PaO was characterised under several chemical and physical treatments. RESULTS: A fragment of a gene encoding a PaO from broccoli (BoPaO) was cloned. The expression of BoPaO showed an important increment during postharvest senescence, in correlation with chlorophyll degradation. Furthermore, broccoli heads were treated with the hormones cytokinin and ethylene. Cytokinin delayed the increment in BoPaO expression, while ethylene accelerated the process. Also, several postharvest treatments were applied in order to evaluate their effect on BoPaO expression. Samples treated with modified atmosphere, hot air, UV‐C or white light showed a delay in chlorophyll degradation and de‐greening. In most cases the treatments also delayed the increment in BoPaO expression during senescence. CONCLUSION: A close correlation between chlorophyll degradation and BoPaO expression was found during broccoli senescence. This relationship was corroborated in samples treated with different hormonal and physical applications. Copyright © 2011 Society of Chemical IndustryFil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaJohn Wiley & Sons Ltd2011-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75246Gómez Lobato, María Eugenia; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 92; 1; 6-7-2011; 151-1580022-51421097-0010CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jsfa.4555info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.4555/pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:49Zoai:ri.conicet.gov.ar:11336/75246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:49.766CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
title Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
spellingShingle Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
Gómez Lobato, María Eugenia
Broccoli
Senescence
Chlorophyll Degradation
Pheophorbide a Oxygenase
title_short Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
title_full Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
title_fullStr Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
title_full_unstemmed Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
title_sort Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.
dc.creator.none.fl_str_mv Gómez Lobato, María Eugenia
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author Gómez Lobato, María Eugenia
author_facet Gómez Lobato, María Eugenia
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author_role author
author2 Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv Broccoli
Senescence
Chlorophyll Degradation
Pheophorbide a Oxygenase
topic Broccoli
Senescence
Chlorophyll Degradation
Pheophorbide a Oxygenase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BACKGROUND: Broccoli is a highly perishable vegetable that shows enhanced postharvest senescence and intense de‐greening caused by chlorophyll degradation. One of the key steps of chlorophyll catabolism is the opening of chlorophyll tretrapyrrole catalysed by pheophorbide a oxygenase (PaO). In this study the expression of a gene encoding a putative PaO was characterised under several chemical and physical treatments. RESULTS: A fragment of a gene encoding a PaO from broccoli (BoPaO) was cloned. The expression of BoPaO showed an important increment during postharvest senescence, in correlation with chlorophyll degradation. Furthermore, broccoli heads were treated with the hormones cytokinin and ethylene. Cytokinin delayed the increment in BoPaO expression, while ethylene accelerated the process. Also, several postharvest treatments were applied in order to evaluate their effect on BoPaO expression. Samples treated with modified atmosphere, hot air, UV‐C or white light showed a delay in chlorophyll degradation and de‐greening. In most cases the treatments also delayed the increment in BoPaO expression during senescence. CONCLUSION: A close correlation between chlorophyll degradation and BoPaO expression was found during broccoli senescence. This relationship was corroborated in samples treated with different hormonal and physical applications. Copyright © 2011 Society of Chemical Industry
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description BACKGROUND: Broccoli is a highly perishable vegetable that shows enhanced postharvest senescence and intense de‐greening caused by chlorophyll degradation. One of the key steps of chlorophyll catabolism is the opening of chlorophyll tretrapyrrole catalysed by pheophorbide a oxygenase (PaO). In this study the expression of a gene encoding a putative PaO was characterised under several chemical and physical treatments. RESULTS: A fragment of a gene encoding a PaO from broccoli (BoPaO) was cloned. The expression of BoPaO showed an important increment during postharvest senescence, in correlation with chlorophyll degradation. Furthermore, broccoli heads were treated with the hormones cytokinin and ethylene. Cytokinin delayed the increment in BoPaO expression, while ethylene accelerated the process. Also, several postharvest treatments were applied in order to evaluate their effect on BoPaO expression. Samples treated with modified atmosphere, hot air, UV‐C or white light showed a delay in chlorophyll degradation and de‐greening. In most cases the treatments also delayed the increment in BoPaO expression during senescence. CONCLUSION: A close correlation between chlorophyll degradation and BoPaO expression was found during broccoli senescence. This relationship was corroborated in samples treated with different hormonal and physical applications. Copyright © 2011 Society of Chemical Industry
publishDate 2011
dc.date.none.fl_str_mv 2011-07-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75246
Gómez Lobato, María Eugenia; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 92; 1; 6-7-2011; 151-158
0022-5142
1097-0010
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75246
identifier_str_mv Gómez Lobato, María Eugenia; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effects of ethylene, cytokinin, and physical treatments on BoPaO gene expression of harvested broccoli.; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 92; 1; 6-7-2011; 151-158
0022-5142
1097-0010
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jsfa.4555
info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.4555/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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