Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"

Autores
Gomez, Mariano Facundo; Carrión, Cristian Antonio; Costa, M.lorenza; Desel, Christine; Kieselbach, Thomas; Funk, Christiane; Krupinska, Karin; Guiamet, Juan José
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlorophyll (Chl) loss is the main visible symptom of senescence in leaves. The initial steps of Chl degradation operate within the chloroplast, but the observation that ‘senescence-associated vacuoles’ (SAVs) contain Chl raises the question of whether SAVs might also contribute to Chl breakdown. Previous confocal microscope observations (Mart ınez et al., 2008) showed many SAVs containing Chl. Isolated SAVs contained Chl a and b (with a Chl a/b ratio close to 5) and lower levels of chlorophyllide a. Pheophytin a and pheophorbide a were formed after the incubation of SAVs at 30°C in darkness, suggesting the presence of Chl-degrading activities in SAVs. Chl in SAVs was bound to a number of ‘green bands’. In the most abundant green band of SAVs, Western blot analysis showed the presence of photosystem I (PSI) Chl-binding proteins, including the PsaA protein of the PSI reaction center and the apoproteins of the light-harvesting complexes (Lhca 1–4). This was confirmed by: (i) measurements of 77-K fluorescence emission spectra showing a single emission peak at around 730 nm in SAVs; (ii) mass spectrometry of the most prominent green band with the slowest electrophoretic mobility; and (iii) immunofluorescence detection of PsaA in SAVs observed through confocal microscopy. Incubation of SAVs at 30°C in darkness caused a steady decrease in PsaA levels. Overall, these results indicate that SAVs may be involved in the degradation of PSI proteins and their associated chlorophylls during the senescence of leaves.
Fil: Gomez, Mariano Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Costa, M.lorenza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Desel, Christine. Christian Albrechts Universität zu Kiel; Alemania
Fil: Kieselbach, Thomas. Umeä University; Suecia
Fil: Funk, Christiane. Umeä University; Suecia
Fil: Krupinska, Karin. Christian Albrechts Universität zu Kiel; Alemania
Fil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
CHLOROPHYLL DEGRADATION
NICOTIANA TABACUM
PHOTOSYSTEM I
SENESCENCE-ASSOCIATED VACUOLES
TOBACCO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122536
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/122536
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"Gomez, Mariano FacundoCarrión, Cristian AntonioCosta, M.lorenzaDesel, ChristineKieselbach, ThomasFunk, ChristianeKrupinska, KarinGuiamet, Juan JoséCHLOROPHYLL DEGRADATIONNICOTIANA TABACUMPHOTOSYSTEM ISENESCENCE-ASSOCIATED VACUOLESTOBACCOhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Chlorophyll (Chl) loss is the main visible symptom of senescence in leaves. The initial steps of Chl degradation operate within the chloroplast, but the observation that ‘senescence-associated vacuoles’ (SAVs) contain Chl raises the question of whether SAVs might also contribute to Chl breakdown. Previous confocal microscope observations (Mart ınez et al., 2008) showed many SAVs containing Chl. Isolated SAVs contained Chl a and b (with a Chl a/b ratio close to 5) and lower levels of chlorophyllide a. Pheophytin a and pheophorbide a were formed after the incubation of SAVs at 30°C in darkness, suggesting the presence of Chl-degrading activities in SAVs. Chl in SAVs was bound to a number of ‘green bands’. In the most abundant green band of SAVs, Western blot analysis showed the presence of photosystem I (PSI) Chl-binding proteins, including the PsaA protein of the PSI reaction center and the apoproteins of the light-harvesting complexes (Lhca 1–4). This was confirmed by: (i) measurements of 77-K fluorescence emission spectra showing a single emission peak at around 730 nm in SAVs; (ii) mass spectrometry of the most prominent green band with the slowest electrophoretic mobility; and (iii) immunofluorescence detection of PsaA in SAVs observed through confocal microscopy. Incubation of SAVs at 30°C in darkness caused a steady decrease in PsaA levels. Overall, these results indicate that SAVs may be involved in the degradation of PSI proteins and their associated chlorophylls during the senescence of leaves.Fil: Gomez, Mariano Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Costa, M.lorenza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Desel, Christine. Christian Albrechts Universität zu Kiel; AlemaniaFil: Kieselbach, Thomas. Umeä University; SueciaFil: Funk, Christiane. Umeä University; SueciaFil: Krupinska, Karin. Christian Albrechts Universität zu Kiel; AlemaniaFil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaWiley Blackwell Publishing, Inc2019-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122536Gomez, Mariano Facundo; Carrión, Cristian Antonio; Costa, M.lorenza; Desel, Christine; Kieselbach, Thomas; et al.; Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"; Wiley Blackwell Publishing, Inc; Plant Journal; 99; 3; 15-4-2019; 465-4770960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14337info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.14337info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:49Zoai:ri.conicet.gov.ar:11336/122536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:49.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
title Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
spellingShingle Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
Gomez, Mariano Facundo
CHLOROPHYLL DEGRADATION
NICOTIANA TABACUM
PHOTOSYSTEM I
SENESCENCE-ASSOCIATED VACUOLES
TOBACCO
title_short Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
title_full Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
title_fullStr Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
title_full_unstemmed Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
title_sort Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"
dc.creator.none.fl_str_mv Gomez, Mariano Facundo
Carrión, Cristian Antonio
Costa, M.lorenza
Desel, Christine
Kieselbach, Thomas
Funk, Christiane
Krupinska, Karin
Guiamet, Juan José
author Gomez, Mariano Facundo
author_facet Gomez, Mariano Facundo
Carrión, Cristian Antonio
Costa, M.lorenza
Desel, Christine
Kieselbach, Thomas
Funk, Christiane
Krupinska, Karin
Guiamet, Juan José
author_role author
author2 Carrión, Cristian Antonio
Costa, M.lorenza
Desel, Christine
Kieselbach, Thomas
Funk, Christiane
Krupinska, Karin
Guiamet, Juan José
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHLOROPHYLL DEGRADATION
NICOTIANA TABACUM
PHOTOSYSTEM I
SENESCENCE-ASSOCIATED VACUOLES
TOBACCO
topic CHLOROPHYLL DEGRADATION
NICOTIANA TABACUM
PHOTOSYSTEM I
SENESCENCE-ASSOCIATED VACUOLES
TOBACCO
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Chlorophyll (Chl) loss is the main visible symptom of senescence in leaves. The initial steps of Chl degradation operate within the chloroplast, but the observation that ‘senescence-associated vacuoles’ (SAVs) contain Chl raises the question of whether SAVs might also contribute to Chl breakdown. Previous confocal microscope observations (Mart ınez et al., 2008) showed many SAVs containing Chl. Isolated SAVs contained Chl a and b (with a Chl a/b ratio close to 5) and lower levels of chlorophyllide a. Pheophytin a and pheophorbide a were formed after the incubation of SAVs at 30°C in darkness, suggesting the presence of Chl-degrading activities in SAVs. Chl in SAVs was bound to a number of ‘green bands’. In the most abundant green band of SAVs, Western blot analysis showed the presence of photosystem I (PSI) Chl-binding proteins, including the PsaA protein of the PSI reaction center and the apoproteins of the light-harvesting complexes (Lhca 1–4). This was confirmed by: (i) measurements of 77-K fluorescence emission spectra showing a single emission peak at around 730 nm in SAVs; (ii) mass spectrometry of the most prominent green band with the slowest electrophoretic mobility; and (iii) immunofluorescence detection of PsaA in SAVs observed through confocal microscopy. Incubation of SAVs at 30°C in darkness caused a steady decrease in PsaA levels. Overall, these results indicate that SAVs may be involved in the degradation of PSI proteins and their associated chlorophylls during the senescence of leaves.
Fil: Gomez, Mariano Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Costa, M.lorenza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Desel, Christine. Christian Albrechts Universität zu Kiel; Alemania
Fil: Kieselbach, Thomas. Umeä University; Suecia
Fil: Funk, Christiane. Umeä University; Suecia
Fil: Krupinska, Karin. Christian Albrechts Universität zu Kiel; Alemania
Fil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description Chlorophyll (Chl) loss is the main visible symptom of senescence in leaves. The initial steps of Chl degradation operate within the chloroplast, but the observation that ‘senescence-associated vacuoles’ (SAVs) contain Chl raises the question of whether SAVs might also contribute to Chl breakdown. Previous confocal microscope observations (Mart ınez et al., 2008) showed many SAVs containing Chl. Isolated SAVs contained Chl a and b (with a Chl a/b ratio close to 5) and lower levels of chlorophyllide a. Pheophytin a and pheophorbide a were formed after the incubation of SAVs at 30°C in darkness, suggesting the presence of Chl-degrading activities in SAVs. Chl in SAVs was bound to a number of ‘green bands’. In the most abundant green band of SAVs, Western blot analysis showed the presence of photosystem I (PSI) Chl-binding proteins, including the PsaA protein of the PSI reaction center and the apoproteins of the light-harvesting complexes (Lhca 1–4). This was confirmed by: (i) measurements of 77-K fluorescence emission spectra showing a single emission peak at around 730 nm in SAVs; (ii) mass spectrometry of the most prominent green band with the slowest electrophoretic mobility; and (iii) immunofluorescence detection of PsaA in SAVs observed through confocal microscopy. Incubation of SAVs at 30°C in darkness caused a steady decrease in PsaA levels. Overall, these results indicate that SAVs may be involved in the degradation of PSI proteins and their associated chlorophylls during the senescence of leaves.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122536
Gomez, Mariano Facundo; Carrión, Cristian Antonio; Costa, M.lorenza; Desel, Christine; Kieselbach, Thomas; et al.; Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"; Wiley Blackwell Publishing, Inc; Plant Journal; 99; 3; 15-4-2019; 465-477
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122536
identifier_str_mv Gomez, Mariano Facundo; Carrión, Cristian Antonio; Costa, M.lorenza; Desel, Christine; Kieselbach, Thomas; et al.; Extra-plastidial degradation of chlorophyll and photosystem I in tobacco leaves involving "Senescence-associated- vacuoles"; Wiley Blackwell Publishing, Inc; Plant Journal; 99; 3; 15-4-2019; 465-477
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14337
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.14337
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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