Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica olera...

Autores
Costa, María Lucía; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo A.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Effects of ethephon and 6-benzylaminopurine (BAP) treatments on enzyme degrading enzymes and a peroxidase-linked chlorophyll bleaching were investigated in broccoli (Brassica oleracea L.) florets. The florets were dipped in solutions containing either BAP or ethephon and then incubated in darkness at 20 ◦C. The hue angle values and chlorophyll contents of ethephontreated florets declined the most of the five-day experiment. In contrast, hue angle and chlorophyll content declined the least in BAP-treated florets. Pheophytin levels increased the most in ethephon-treated florets and the least in BAP-treated florets. Chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching increased over the five days in control (untreated) florets. Ethephon treatment enhanced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels as compared with control. BAP treatment reduced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels. It was concluded that the activities of chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching could be regulated by external application of either ethylene (applied as ethephon) or a cytokinin (applied as BAP). In the case of ethephon treatment, accelerated rates of chlorophyll degradation were found and in the case of BAP-treatment, reduced rates of chlorophyll degradation were found.
Fil: Costa, María Lucía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Martínez, Gustavo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
BROCCOLI
CHLOROPHYLL DEGRADATION
ETHEPHON
BAP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/240657
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/240657
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°CCosta, María LucíaCivello, Pedro MarcosChaves, Alicia RaquelMartínez, Gustavo A.BROCCOLICHLOROPHYLL DEGRADATIONETHEPHONBAPhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Effects of ethephon and 6-benzylaminopurine (BAP) treatments on enzyme degrading enzymes and a peroxidase-linked chlorophyll bleaching were investigated in broccoli (Brassica oleracea L.) florets. The florets were dipped in solutions containing either BAP or ethephon and then incubated in darkness at 20 ◦C. The hue angle values and chlorophyll contents of ethephontreated florets declined the most of the five-day experiment. In contrast, hue angle and chlorophyll content declined the least in BAP-treated florets. Pheophytin levels increased the most in ethephon-treated florets and the least in BAP-treated florets. Chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching increased over the five days in control (untreated) florets. Ethephon treatment enhanced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels as compared with control. BAP treatment reduced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels. It was concluded that the activities of chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching could be regulated by external application of either ethylene (applied as ethephon) or a cytokinin (applied as BAP). In the case of ethephon treatment, accelerated rates of chlorophyll degradation were found and in the case of BAP-treatment, reduced rates of chlorophyll degradation were found.Fil: Costa, María Lucía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Martínez, Gustavo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaElsevier Science2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240657Costa, María Lucía; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo A.; Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C; Elsevier Science; Postharvest Biology and Technology; 35; 2; 2-2005; 191-1990925-5214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925521404002030info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2004.07.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:52Zoai:ri.conicet.gov.ar:11336/240657instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:52.741CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
title Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
spellingShingle Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
Costa, María Lucía
BROCCOLI
CHLOROPHYLL DEGRADATION
ETHEPHON
BAP
title_short Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
title_full Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
title_fullStr Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
title_full_unstemmed Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
title_sort Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C
dc.creator.none.fl_str_mv Costa, María Lucía
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo A.
author Costa, María Lucía
author_facet Costa, María Lucía
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo A.
author_role author
author2 Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo A.
author2_role author
author
author
dc.subject.none.fl_str_mv BROCCOLI
CHLOROPHYLL DEGRADATION
ETHEPHON
BAP
topic BROCCOLI
CHLOROPHYLL DEGRADATION
ETHEPHON
BAP
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Effects of ethephon and 6-benzylaminopurine (BAP) treatments on enzyme degrading enzymes and a peroxidase-linked chlorophyll bleaching were investigated in broccoli (Brassica oleracea L.) florets. The florets were dipped in solutions containing either BAP or ethephon and then incubated in darkness at 20 ◦C. The hue angle values and chlorophyll contents of ethephontreated florets declined the most of the five-day experiment. In contrast, hue angle and chlorophyll content declined the least in BAP-treated florets. Pheophytin levels increased the most in ethephon-treated florets and the least in BAP-treated florets. Chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching increased over the five days in control (untreated) florets. Ethephon treatment enhanced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels as compared with control. BAP treatment reduced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels. It was concluded that the activities of chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching could be regulated by external application of either ethylene (applied as ethephon) or a cytokinin (applied as BAP). In the case of ethephon treatment, accelerated rates of chlorophyll degradation were found and in the case of BAP-treatment, reduced rates of chlorophyll degradation were found.
Fil: Costa, María Lucía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Martínez, Gustavo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description Effects of ethephon and 6-benzylaminopurine (BAP) treatments on enzyme degrading enzymes and a peroxidase-linked chlorophyll bleaching were investigated in broccoli (Brassica oleracea L.) florets. The florets were dipped in solutions containing either BAP or ethephon and then incubated in darkness at 20 ◦C. The hue angle values and chlorophyll contents of ethephontreated florets declined the most of the five-day experiment. In contrast, hue angle and chlorophyll content declined the least in BAP-treated florets. Pheophytin levels increased the most in ethephon-treated florets and the least in BAP-treated florets. Chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching increased over the five days in control (untreated) florets. Ethephon treatment enhanced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels as compared with control. BAP treatment reduced chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching levels. It was concluded that the activities of chlorophyllase, Mg-dechelatase, and peroxidase-linked chlorophyll bleaching could be regulated by external application of either ethylene (applied as ethephon) or a cytokinin (applied as BAP). In the case of ethephon treatment, accelerated rates of chlorophyll degradation were found and in the case of BAP-treatment, reduced rates of chlorophyll degradation were found.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240657
Costa, María Lucía; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo A.; Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C; Elsevier Science; Postharvest Biology and Technology; 35; 2; 2-2005; 191-199
0925-5214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240657
identifier_str_mv Costa, María Lucía; Civello, Pedro Marcos; Chaves, Alicia Raquel; Martínez, Gustavo A.; Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C; Elsevier Science; Postharvest Biology and Technology; 35; 2; 2-2005; 191-199
0925-5214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925521404002030
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2004.07.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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