Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein

Autores
Robles Luna, Gabriel; Peña, Eduardo José; Borniego, María Belén; Heinlein, Manfred; García, María Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally trans-complement cell-to-cell movement-defective Potato virus X (PVX) and Tobacco mosaic virus (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Movement protein
Negative-strand RNA virus
Ophioviridae
Protein-protein Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85543
Acceder
id SEDICI_37285008fe060a67ba8fd2588b56d039
oai_identifier_str oai:sedici.unlp.edu.ar:10915/85543
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat proteinRobles Luna, GabrielPeña, Eduardo JoséBorniego, María BelénHeinlein, ManfredGarcía, María LauraCiencias ExactasCitrus psorosis virusMirafiori lettuce big-vein virusMovement proteinNegative-strand RNA virusOphioviridaeProtein-protein Interaction<i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf152-161http://sedici.unlp.edu.ar/handle/10915/85543enginfo:eu-repo/semantics/altIdentifier/issn/0042-6822info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2013.03.019info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:26Zoai:sedici.unlp.edu.ar:10915/85543Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:26.543SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
spellingShingle Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
Robles Luna, Gabriel
Ciencias Exactas
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Movement protein
Negative-strand RNA virus
Ophioviridae
Protein-protein Interaction
title_short Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_full Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_fullStr Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_full_unstemmed Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_sort Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
dc.creator.none.fl_str_mv Robles Luna, Gabriel
Peña, Eduardo José
Borniego, María Belén
Heinlein, Manfred
García, María Laura
author Robles Luna, Gabriel
author_facet Robles Luna, Gabriel
Peña, Eduardo José
Borniego, María Belén
Heinlein, Manfred
García, María Laura
author_role author
author2 Peña, Eduardo José
Borniego, María Belén
Heinlein, Manfred
García, María Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Movement protein
Negative-strand RNA virus
Ophioviridae
Protein-protein Interaction
topic Ciencias Exactas
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Movement protein
Negative-strand RNA virus
Ophioviridae
Protein-protein Interaction
dc.description.none.fl_txt_mv <i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description <i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85543
url http://sedici.unlp.edu.ar/handle/10915/85543
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0042-6822
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2013.03.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
152-161
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.221938

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