<i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo

Autores
Peña, Eduardo José; Robles Luna, Gabriel; Zanek, María Cecilia; Borniego, María Belén; Reyes, Carina Andrea; Heinlein, Manfred; García, María Laura
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/112878
Acceder
id SEDICI_29cc16e35e87b1e041c36e25e0871826
oai_identifier_str oai:sedici.unlp.edu.ar:10915/112878
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivoPeña, Eduardo JoséRobles Luna, GabrielZanek, María CeciliaBorniego, María BelénReyes, Carina AndreaHeinlein, ManfredGarcía, María LauraBiologíaCitrus psorosis virusMirafiori lettuce big-vein virusOphioviridaeCoat proteinProtein interactionFRETFLIMImmunoprecipitationSubcellular localizationCitrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.Instituto de Biotecnologia y Biologia Molecular2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf34-43http://sedici.unlp.edu.ar/handle/10915/112878enginfo:eu-repo/semantics/altIdentifier/issn/0168-1702info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2012.08.005info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:18:09Zoai:sedici.unlp.edu.ar:10915/112878Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:18:09.784SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
spellingShingle <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
Peña, Eduardo José
Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
title_short <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_full <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_fullStr <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_full_unstemmed <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
title_sort <i>Citrus psorosis</i> and <i>Mirafiori lettuce big-vein</i> ophiovirus coat proteins localize to the cytoplasm and self interact in vivo
dc.creator.none.fl_str_mv Peña, Eduardo José
Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
author Peña, Eduardo José
author_facet Peña, Eduardo José
Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
author_role author
author2 Robles Luna, Gabriel
Zanek, María Cecilia
Borniego, María Belén
Reyes, Carina Andrea
Heinlein, Manfred
García, María Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
topic Biología
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Ophioviridae
Coat protein
Protein interaction
FRET
FLIM
Immunoprecipitation
Subcellular localization
dc.description.none.fl_txt_mv Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
Instituto de Biotecnologia y Biologia Molecular
description Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/112878
url http://sedici.unlp.edu.ar/handle/10915/112878
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0168-1702
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2012.08.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
34-43
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1846064239703228416
score 13.22299

Publicaciones similares