Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein

Autores
Robles Luna, Gabriel; Peña, Eduardojosé; Borniego, María Belén; Heinlein, Manfred; Garcia, Maria Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally trans-complement cell-tocell movement-defective Potato virus X (PVX) and Tobacco mosaic virus (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Peña, Eduardojosé. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; Francia
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Ophioviridae
Negative-Strand Rna Virus
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
Movement Protein
Protein–Protein Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24506
Acceder
id CONICETDig_8bdef423cf0c590cf29b4986184ce358
oai_identifier_str oai:ri.conicet.gov.ar:11336/24506
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat proteinRobles Luna, GabrielPeña, EduardojoséBorniego, María BelénHeinlein, ManfredGarcia, Maria LauraOphioviridaeNegative-Strand Rna VirusCitrus Psorosis VirusMirafiori Lettuce Big-Vein VirusMovement ProteinProtein–Protein Interactionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally trans-complement cell-tocell movement-defective Potato virus X (PVX) and Tobacco mosaic virus (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Peña, Eduardojosé. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; FranciaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAcademic Press Inc Elsevier Science2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24506Robles Luna, Gabriel; Peña, Eduardojosé; Borniego, María Belén; Heinlein, Manfred; Garcia, Maria Laura; Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein; Academic Press Inc Elsevier Science; Virology; 441; 2; 7-2013; 152-1610042-6822CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0042682213001724info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2013.03.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:30:35Zoai:ri.conicet.gov.ar:11336/24506instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:30:35.422CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
title Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
spellingShingle Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
Robles Luna, Gabriel
Ophioviridae
Negative-Strand Rna Virus
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
Movement Protein
Protein–Protein Interaction
title_short Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
title_full Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
title_fullStr Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
title_full_unstemmed Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
title_sort Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein
dc.creator.none.fl_str_mv Robles Luna, Gabriel
Peña, Eduardojosé
Borniego, María Belén
Heinlein, Manfred
Garcia, Maria Laura
author Robles Luna, Gabriel
author_facet Robles Luna, Gabriel
Peña, Eduardojosé
Borniego, María Belén
Heinlein, Manfred
Garcia, Maria Laura
author_role author
author2 Peña, Eduardojosé
Borniego, María Belén
Heinlein, Manfred
Garcia, Maria Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ophioviridae
Negative-Strand Rna Virus
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
Movement Protein
Protein–Protein Interaction
topic Ophioviridae
Negative-Strand Rna Virus
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
Movement Protein
Protein–Protein Interaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally trans-complement cell-tocell movement-defective Potato virus X (PVX) and Tobacco mosaic virus (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Peña, Eduardojosé. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moleculaire Des Plantes; Francia
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally trans-complement cell-tocell movement-defective Potato virus X (PVX) and Tobacco mosaic virus (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24506
Robles Luna, Gabriel; Peña, Eduardojosé; Borniego, María Belén; Heinlein, Manfred; Garcia, Maria Laura; Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein; Academic Press Inc Elsevier Science; Virology; 441; 2; 7-2013; 152-161
0042-6822
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24506
identifier_str_mv Robles Luna, Gabriel; Peña, Eduardojosé; Borniego, María Belén; Heinlein, Manfred; Garcia, Maria Laura; Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA2 and interacts with the coat protein; Academic Press Inc Elsevier Science; Virology; 441; 2; 7-2013; 152-161
0042-6822
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0042682213001724
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2013.03.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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