Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily

Autores
Borniego, María Belén; Karlin, David; Peña, Eduardo José; Robles Luna, Gabriel; Garcia, Maria Laura
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MPMiLBVV, while MPCPsV was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Karlin, David. University of Oxford; Reino Unido. Henry Wellcome Building; Reino Unido
Fil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Movement Protein
30k Superfamily
Ophiovirus
Negative-Strand Rna Virus
Tmv Vector
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47926
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47926
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamilyBorniego, María BelénKarlin, DavidPeña, Eduardo JoséRobles Luna, GabrielGarcia, Maria LauraMovement Protein30k SuperfamilyOphiovirusNegative-Strand Rna VirusTmv VectorCitrus Psorosis VirusMirafiori Lettuce Big-Vein Virushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MPMiLBVV, while MPCPsV was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Karlin, David. University of Oxford; Reino Unido. Henry Wellcome Building; Reino UnidoFil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAcademic Press Inc Elsevier Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47926Borniego, María Belén; Karlin, David; Peña, Eduardo José; Robles Luna, Gabriel; Garcia, Maria Laura; Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily; Academic Press Inc Elsevier Science; Virology; 498; 11-2016; 172-1800042-6822CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2016.08.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0042682216302434info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:09Zoai:ri.conicet.gov.ar:11336/47926instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:09.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
spellingShingle Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
Borniego, María Belén
Movement Protein
30k Superfamily
Ophiovirus
Negative-Strand Rna Virus
Tmv Vector
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
title_short Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_full Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_fullStr Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_full_unstemmed Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_sort Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
dc.creator.none.fl_str_mv Borniego, María Belén
Karlin, David
Peña, Eduardo José
Robles Luna, Gabriel
Garcia, Maria Laura
author Borniego, María Belén
author_facet Borniego, María Belén
Karlin, David
Peña, Eduardo José
Robles Luna, Gabriel
Garcia, Maria Laura
author_role author
author2 Karlin, David
Peña, Eduardo José
Robles Luna, Gabriel
Garcia, Maria Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Movement Protein
30k Superfamily
Ophiovirus
Negative-Strand Rna Virus
Tmv Vector
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
topic Movement Protein
30k Superfamily
Ophiovirus
Negative-Strand Rna Virus
Tmv Vector
Citrus Psorosis Virus
Mirafiori Lettuce Big-Vein Virus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MPMiLBVV, while MPCPsV was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Karlin, David. University of Oxford; Reino Unido. Henry Wellcome Building; Reino Unido
Fil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MPMiLBVV, while MPCPsV was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47926
Borniego, María Belén; Karlin, David; Peña, Eduardo José; Robles Luna, Gabriel; Garcia, Maria Laura; Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily; Academic Press Inc Elsevier Science; Virology; 498; 11-2016; 172-180
0042-6822
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47926
identifier_str_mv Borniego, María Belén; Karlin, David; Peña, Eduardo José; Robles Luna, Gabriel; Garcia, Maria Laura; Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily; Academic Press Inc Elsevier Science; Virology; 498; 11-2016; 172-180
0042-6822
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2016.08.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0042682216302434
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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